(Circular) (Six-base) MAPSORT of: pFN21AE5963.seq Check: 4086 from: 1 to: 5641 pFN21AE5963 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2103 4540 5517 1203 Size: 900 2437 977 1327 Fragments arranged by size: 2437 1327 977 900 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2252 2414 1166 Size: 1086 162 4393 Fragments arranged by size: 4393 1086 162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2760 3384 4956 2760 Size: 624 1572 3445 Fragments arranged by size: 3445 1572 624 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2011 2208 2330 2679 2950 3176 3328 5437 Size: 518 197 122 349 271 226 152 2109 Cuts at: 5437 1493 Size: 1697 Fragments arranged by size: 2109 1697 518 349 271 226 197 152 122 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2960 1391 Size: 1569 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 2127 2460 2521 1548 Size: 579 333 61 4668 Fragments arranged by size: 4668 579 333 61 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2295 3058 2295 Size: 763 4878 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 2220 1318 Size: 902 4739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2127 3372 4330 Size: 416 53 83 399 24 710 1245 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1245 958 710 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 2132 2157 5635 2132 Size: 25 3478 2138 Fragments arranged by size: 3478 2138 25 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2664 4074 4472 4746 4893 5304 2664 Size: 1410 398 274 147 411 3001 Fragments arranged by size: 3001 1410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2808 2838 1178 Size: 30 165 30 1405 30 3981 Fragments arranged by size: 3981 1405 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2713 2835 1186 Size: 1527 122 3992 Fragments arranged by size: 3992 1527 122 BseYI C'CCAG_C Cuts at: 4844 5340 4844 Size: 496 5145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2411 2829 3702 3787 4858 2411 Size: 418 873 85 1071 3194 Fragments arranged by size: 3194 1071 873 418 85 BsmI GAATG_Cn' Cuts at: 2229 3152 3245 2229 Size: 923 93 4625 Fragments arranged by size: 4625 923 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2411 2829 2960 3702 3787 4858 1391 Size: 399 621 418 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 418 399 131 85 BspEI T'CCGG_A Cuts at: 2664 5304 2664 Size: 2640 3001 BspHI T'CATG_A Cuts at: 2403 3530 5260 2403 Size: 1127 1730 2784 Fragments arranged by size: 2784 1730 1127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2996 844 Size: 1215 937 3489 Fragments arranged by size: 3489 1215 937 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 2772 96 Size: 2676 2965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4202 BstYI r'GATC_y Cuts at: 1972 2152 2971 3338 3723 3740 5192 5278 5290 Size: 180 819 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 819 385 367 346 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5089 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2934 2945 2934 Size: 11 5630 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 3088 3977 Size: 54 1425 50 275 18 540 718 889 Cuts at: 3977 5606 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 718 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2391 2545 3571 1353 Size: 1038 154 1026 3423 Fragments arranged by size: 3423 1038 1026 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3704 4301 5088 5356 1239 Size: 393 2072 597 787 268 1524 Fragments arranged by size: 2072 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2820 2914 2820 Size: 94 5547 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2229 2261 4282 4314 4356 Size: 32 135 32 657 32 2021 32 42 Cuts at: 4356 4388 1373 Size: 32 2626 Fragments arranged by size: 2626 2021 657 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 4755 4939 1305 Size: 1170 2280 184 2007 Fragments arranged by size: 2280 2007 1170 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 10 Size: 54 1425 343 540 3279 Fragments arranged by size: 3279 1425 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2414 3599 3958 4117 518 Size: 648 1086 162 1185 359 159 2042 Fragments arranged by size: 2042 1185 1086 648 359 162 159 MspA1I CmG'CkG Cuts at: 1606 2364 3057 3734 4882 5127 1606 Size: 758 693 677 1148 245 2120 Fragments arranged by size: 2120 1148 758 693 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5089 NdeI CA'TA_TG Cuts at: 387 2730 387 Size: 2343 3298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2107 2999 4544 1132 Size: 97 878 892 1545 2229 Fragments arranged by size: 2229 1545 892 878 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 2103 4540 2103 Size: 2437 3204 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2202 2202 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 2280 3211 2280 Size: 931 4710 PstI C_TGCA'G Cuts at: 830 2166 2493 2556 2993 830 Size: 1336 327 63 437 3478 Fragments arranged by size: 3478 1336 437 327 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2162 2489 2552 2989 4124 4805 4996 Size: 219 1117 327 63 437 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1135 1117 681 437 327 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 3080 513 Size: 552 2015 3074 Fragments arranged by size: 3074 2015 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2274 3668 3685 3838 4023 4468 325 Size: 1255 694 1394 17 153 185 445 1498 Fragments arranged by size: 1498 1394 1255 694 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2074 2772 3887 Size: 274 80 33 51 1443 97 698 1115 Cuts at: 3887 5599 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1115 698 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 5601 5633 2808 Size: 32 2761 32 2816 Fragments arranged by size: 2816 2761 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2581 2977 2581 Size: 396 5245 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 2272 2858 3770 2272 Size: 586 912 4143 Fragments arranged by size: 4143 912 586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI