################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BpmI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BsrBI BsrDI BsrFI BssHII BssKI BssSI BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MroI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 224 GTATAC GT^[AC][GT]AC AciI 357 CCGC CCGC(-3/-1) AfaI 768 GTAC GT^AC AflIII 712 ACATGT A^C[GA][CT]GT AseI 630 ATTAAT AT^TAAT AsnI 630 ATTAAT AT^TAAT Asp700I 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 365 TGGCCA TGG^CCA BbvCI 779 CCTCAGC CCTCAGC(-5/-2) BcgI 406 CGAAGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 686 CTAG C^TAG BlpI 102 GCTCAGC GC^T[ACGT]AGC Bpu1102I 102 GCTCAGC GC^T[ACGT]AGC BsgI 414 CTGCAC GTGCAG(16/14) BsiYI 357 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 357 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1407I 767 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BspLU11I 712 ACATGT A^CATGT BspMI 155 ACCTGC ACCTGC(4/8) BsrGI 767 TGTACA T^GTACA BsrI 112 ACTGG ACTGG(1/-1) Bst1107I 224 GTATAC GTA^TAC BstXI 230 CCAAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 224 GTATAC GTA^TAC CelII 102 GCTCAGC GC^T[ACGT]AGC DraI 483 TTTAAA TTT^AAA EaeI 365 TGGCCA [CT]^GGCC[GA] EarI 407 GAAGAG CTCTTC(1/4) EcoRI 674 GAATTC G^AATTC Ksp632I 407 GAAGAG CTCTTC(1/4) MaeI 686 CTAG C^TAG MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA MspA1I 753 CAGCTG C[AC]G^C[GT]G NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC PshBI 630 ATTAAT AT^TAAT PstI 157 CTGCAG CTGCA^G PvuII 753 CAGCTG CAG^CTG RcaI 398 TCATGA T^CATGA RsaI 768 GTAC GT^AC SfaNI 107 GCATC GCATC(5/9) SpeI 685 ACTAGT A^CTAGT SspBI 767 TGTACA T^GTACA StuI 701 AGGCCT AGG^CCT TaqI 405 TCGA T^CGA Tsp45I 319 GTCAC ^GT[GC]AC TthHB8I 405 TCGA T^CGA XmnI 674 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BbsI 912 GAAGAC GAAGAC(2/6) 79 GTCTTC BpuAI 912 GAAGAC GAAGAC(2/6) 79 GTCTTC Cac8I 374 GCAAGC GC[ACGT]^[ACGT]GC 888 GCAAGC HaeIII 366 GGCC GG^CC 702 GGCC HindIII 487 AAGCTT A^AGCTT 890 AAGCTT HphI 582 GGTGA GGTGA(8/7) 92 TCACC MaeIII 242 GTAAC ^GT[ACGT]AC 319 GTCAC MwoI 480 GCTTTTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 879 GCAAGTCAAGC NspI 98 ACATGC [GA]CATG^[CT] 712 ACATGT PleI 317 GAGTC GAGTC(4/5) 410 GAGTC SfcI 157 CTGCAG C^T[GA][CT]AG 498 CTGTAG SmlI 390 CTTGAG C^T[CT][GA]AG 801 CTTGAG SspI 508 AATATT AAT^ATT 633 AATATT TfiI 148 GATTC G^A[AT]TC 402 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AcsI AluI ApoI BbvI DdeI Fnu4HI HinfI ItaI MboII MnlI MseI MslI NlaIII Tru9I TseI Tsp509I TspRI