################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) AcyI 162 GACGCC G[GA]^CG[CT]C AvrII 36 CCTAGG C^CTAGG BamHI 147 GGATCC G^GATCC BbsI 892 GTCTTC GAAGAC(2/6) BciVI 802 GTATCC GTATCC(6/5) BglII 551 AGATCT A^GATCT BlnI 36 CCTAGG C^CTAGG BpuAI 892 GTCTTC GAAGAC(2/6) BsaHI 162 GACGCC G[GA]^CG[CT]C BsiYI 306 CCTTTTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 306 CCTTTTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 480 GGGAC GGGAC(10/14) BspMI 879 GCAGGT ACCTGC(4/8) BstAPI 407 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 336 CCTGG CC^[AT]GG EarI 678 CTCTTC CTCTTC(1/4) EcoRII 336 CCTGG ^CC[AT]GG EcoT14I 36 CCTAGG C^C[AT][AT]GG HgaI 162 GACGC GACGC(5/10) Hin1I 162 GACGCC G[GA]^CG[CT]C Ksp632I 678 CTCTTC CTCTTC(1/4) MaeII 362 ACGT A^CGT MfeI 833 CAATTG C^AATTG MslI 213 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 833 CAATTG C^AATTG MvaI 336 CCTGG CC^[AT]GG NdeI 746 CATATG CA^TATG NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC PleI 482 GACTC GAGTC(4/5) PstI 547 CTGCAG CTGCA^G SfcI 547 CTGCAG C^T[GA][CT]AG SspI 207 AATATT AAT^ATT StyI 36 CCTAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 673 AAATTC [GA]^AATT[CT] 915 GAATTT AfaI 427 GTAC GT^AC 716 GTAC AlwI 148 GATCC GGATC(4/5) 147 GGATC ApoI 673 AAATTC [GA]^AATT[CT] 915 GAATTT BbvI 404 GCAGC GCAGC(8/12) 340 GCTGC BcnI 152 CCCGG CC^[GC]GG 313 CCGGG BsaJI 36 CCTAGG C^C[ACGT][ACGT]GG 151 CCCCGG BsgI 558 CTGCAC GTGCAG(16/14) 683 CTGCAC BsmAI 257 GAGAC GTCTC(1/5) 736 GTCTC BsrI 445 ACTGG ACTGG(1/-1) 624 ACTGG BstF5I 132 CATCC GGATG(2/0) 900 GGATG BstYI 147 GGATCC [GA]^GATC[CT] 551 AGATCT DpnII 148 GATC ^GATC 552 GATC Fnu4HI 340 GCTGC GC^[ACGT]GC 404 GCAGC FokI 132 CATCC GGATG(9/13) 900 GGATG HapII 153 CCGG C^CGG 313 CCGG HpaII 153 CCGG C^CGG 313 CCGG ItaI 340 GCTGC GC^[ACGT]GC 404 GCAGC MboI 148 GATC ^GATC 552 GATC MflI 147 GGATCC [GA]^GATC[CT] 551 AGATCT MspI 153 CCGG C^CGG 313 CCGG MwoI 156 GCTCAAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 407 GCAAAGTCTGC NciI 152 CCCGG CC^[GC]GG 313 CCGGG NdeII 148 GATC ^GATC 552 GATC RsaI 427 GTAC GT^AC 716 GTAC Sau3AI 148 GATC ^GATC 552 GATC TseI 340 GCTGC G^C[AT]GC 404 GCAGC XhoII 147 GGATCC [GA]^GATC[CT] 551 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BfaI BssKI DdeI HinfI HphI MaeI MaeIII MboII MnlI MseI NlaIII ScrFI SmlI TfiI Tru9I Tsp45I Tsp509I TspRI