Restriction map for
pFN21AE5965
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaHI BsaJI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I Cfr13I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MflI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 220 GTCTAC GT^[AC][GT]AC
AciI 466 CCGC CCGC(-3/-1)
AlwI 52 GATCC GGATC(4/5)
AlwNI 563 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 356 AGCGCT AGC^GCT
AseI 582 ATTAAT AT^TAAT
AsnI 582 ATTAAT AT^TAAT
Asp700I 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 365 TGGCCA TGG^CCA
BbvCI 779 CCTCAGC CCTCAGC(-5/-2)
BlpI 822 GCTCAGC GC^T[ACGT]AGC
BpmI 484 CTCCAG CTGGAG(16/14)
Bpu1102I 822 GCTCAGC GC^T[ACGT]AGC
BsaBI 148 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 481 GGTCTC GGTCTC(1/5)
BsiYI 723 CCACATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 723 CCACATGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 398 TCATGA T^CATGA
BssKI 615 CCTGG ^CC[ACGT]GG
BstAPI 407 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 440 GGATG GGATG(2/0)
BstNI 615 CCTGG CC^[AT]GG
CelII 822 GCTCAGC GC^T[ACGT]AGC
CfoI 357 GCGC GCG^C
DraIII 724 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 365 TGGCCA [CT]^GGCC[GA]
Eco47III 356 AGCGCT AGC^GCT
EcoRII 615 CCTGG ^CC[AT]GG
FokI 440 GGATG GGATG(9/13)
HaeII 356 AGCGCT [GA]GCGC^[CT]
HaeIII 366 GGCC GG^CC
HgaI 609 GACGC GACGC(5/10)
HhaI 357 GCGC GCG^C
HinP1I 357 GCGC G^CGC
MaeIII 619 GTTAC ^GT[ACGT]AC
MamI 148 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MvaI 615 CCTGG CC^[AT]GG
MwoI 407 GCAGAGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 723 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 582 ATTAAT AT^TAAT
PstI 547 CTGCAG CTGCA^G
RcaI 398 TCATGA T^CATGA
ScrFI 615 CCTGG CC^[ACGT]GG
SmlI 157 CTCAAG C^T[CT][GA]AG
SspI 652 AATATT AAT^ATT
Van91I 723 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 325 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AflIII 98 ACATGT A^C[GA][CT]GT
712 ACATGT
BsgI 284 GTGCAG GTGCAG(16/14)
500 GTGCAG
BsmAI 205 GTCTC GTCTC(1/5)
482 GTCTC
BspLU11I 98 ACATGT A^CATGT
712 ACATGT
Cac8I 419 GCTTGC GC[ACGT]^[ACGT]GC
445 GCTGGC
DpnII 52 GATC ^GATC
517 GATC
MboI 52 GATC ^GATC
517 GATC
MnlI 180 CCTC CCTC(7/6)
779 CCTC
MspA1I 292 CAGCTG C[AC]G^C[GT]G
753 CAGCTG
NdeII 52 GATC ^GATC
517 GATC
NspI 98 ACATGT [GA]CATG^[CT]
712 ACATGT
PvuII 292 CAGCTG CAG^CTG
753 CAGCTG
Sau3AI 52 GATC ^GATC
517 GATC
SfcI 512 CTGTAG C^T[GA][CT]AG
547 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AcsI AluI ApoI BbvI BsrI
DdeI Fnu4HI HinfI HphI ItaI
MboII MseI MslI NlaIII SfaNI
TfiI Tru9I TseI Tsp509I TspRI