Restriction map for
pFN21AE0962
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AviII BamHI BbvCI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
Ksp632I MamI MfeI MluI MroI
MseI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Van91I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1415 GGTACC G^GTACC
AcyI 236 GGCGCC G[GA]^CG[CT]C
Alw44I 150 GTGCAC G^TGCAC
ApaLI 150 GTGCAC G^TGCAC
Asp718I 1415 GGTACC G^GTACC
AspI 1372 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 768 CCTAGG C^CTAGG
BalI 348 TGGCCA TGG^CCA
BbeI 236 GGCGCC GGCGC^C
BbsI 1458 GAAGAC GAAGAC(2/6)
BlnI 768 CCTAGG C^CTAGG
BpuAI 1458 GAAGAC GAAGAC(2/6)
BsaHI 236 GGCGCC G[GA]^CG[CT]C
BsaI 62 GAGACC GGTCTC(1/5)
BsmBI 77 CGTCTC CGTCTC(1/5)
BsrFI 1358 ACCGGC [GA]^CCGG[CT]
BsrI 479 CCAGT ACTGG(1/-1)
BssSI 838 CACGAG CACGAG(-5/-1)
BstAPI 1157 GCACGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1358 ACCGGC [GA]^CCGG[CT]
DraIII 289 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 914 CCGCGG C^C[GA][CT]GG
HaeII 236 GGCGCC [GA]GCGC^[CT]
HgaI 876 GACGC GACGC(5/10)
Hin1I 236 GGCGCC G[GA]^CG[CT]C
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
KasI 236 GGCGCC G^GCGCC
KpnI 1415 GGTACC GGTAC^C
KspI 914 CCGCGG CCGC^GG
MluNI 348 TGGCCA TGG^CCA
MscI 348 TGGCCA TGG^CCA
MslI 153 CACAGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 236 GGCGCC GG^CGCC
NheI 903 GCTAGC G^CTAGC
PflFI 1372 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 524 GGGACCT [GA]G^G[AT]CC[CT]
SacI 223 GAGCTC GAGCT^C
SacII 914 CCGCGG CCGC^GG
SfoI 236 GGCGCC GGC^GCC
SphI 919 GCATGC GCATG^C
TfiI 1503 GATTC G^A[AT]TC
Tsp509I 1473 AATT ^AATT
Tth111I 1372 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1115 CCAAGCGGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 103 ACGTGT A^C[GA][CT]GT
293 ACGTGT
AlwNI 537 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
874 CAGACGCTG
ApaI 84 GGGCCC GGGCC^C
369 GGGCCC
BbrPI 102 CACGTG CAC^GTG
940 CACGTG
BpmI 307 CTGGAG CTGGAG(16/14)
880 CTGGAG
BsaAI 102 CACGTG [CT]AC^GT[GA]
940 CACGTG
BseRI 693 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsmFI 524 GGGAC GGGAC(10/14)
297 GTCCC
BstEII 51 GGTGACC G^GT[ACGT]ACC
93 GGTGACC
BstPI 51 GGTGACC G^GT[ACGT]ACC
93 GGTGACC
EaeI 348 TGGCCA [CT]^GGCC[GA]
677 TGGCCG
EcoO65I 51 GGTGACC G^GT[ACGT]ACC
93 GGTGACC
PaeR7I 696 CTCGAG C^TCGAG
804 CTCGAG
PmaCI 102 CACGTG CAC^GTG
940 CACGTG
PmlI 102 CACGTG CAC^GTG
940 CACGTG
PvuII 352 CAGCTG CAG^CTG
814 CAGCTG
SfiI 381 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
390 GGCCCTGGTGGCC
SmlI 696 CTCGAG C^T[CT][GA]AG
804 CTCGAG
StuI 24 AGGCCT AGG^CCT
1386 AGGCCT
TaqI 697 TCGA T^CGA
805 TCGA
TthHB8I 697 TCGA T^CGA
805 TCGA
XhoI 696 CTCGAG C^TCGAG
804 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrDI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XhoII