Restriction map for
pFN21AE0962
(Circular) (Six-base) MAPSORT of: pFN21AE0962.seq Check: 1150 from: 1 to: 6214
pFN21AE0962 6214 bp
With 182 enzymes: SgfI *
March 28, 2012 12:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2949 2949
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3420 3535 3420
Size: 115 6099
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4277 5661 5929 1632
Size: 2645 1384 268 1917
Fragments arranged by size:
2645 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2108 2298 5113 6090 1203
Size: 905 190 2815 977 1327
Fragments arranged by size:
2815 1327 977 905 190
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 5218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2180 1184
Size: 996 5218
AlwNI CAG_nnn'CTG
Cuts at: 2547 2884 3957 5529 2547
Size: 337 1073 1572 3232
Fragments arranged by size:
3232 1572 1073 337
ApaI G_GGCC'C
Cuts at: 2093 2378 2093
Size: 285 5929
ApaLI G'TGCA_C
Cuts at: 2155 4271 5427 2155
Size: 2116 1156 2942
Fragments arranged by size:
2942 2116 1156
ApoI r'AATT_y
Cuts at: 1493 3523 3749 3901 6010 1493
Size: 2030 226 152 2109 1697
Fragments arranged by size:
2109 2030 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2701 2809 3106 3137 3539 1870
Size: 73 142 616 108 297 31 402 4545
Fragments arranged by size:
4545 616 402 297 142 108 73 31
AvrII C'CTAG_G
Cuts at: 2773 2773
Size: 6214
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 2241 3116 3133 3289
Size: 325 217 627 400 54 875 17 156
Cuts at: 3289 3420 3535 4990 618
Size: 131 115 1455 1842
Fragments arranged by size:
1842 1455 875 627 400 325 217 156
131 115 54 17
BanII G_rGCy'C
Cuts at: 1391 2018 2093 2120 2232 2378 2574 2892 3533
Size: 627 75 27 112 146 196 318 641
Cuts at: 3533 1391
Size: 4072
Fragments arranged by size:
4072 641 627 318 196 146 112 75
27
BbeI G_GCGC'C
Cuts at: 2245 2245
Size: 6214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3470 928
Size: 971 1571 3672
Fragments arranged by size:
3672 1571 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 2402 2643
Size: 107 122 71 631 605 721 9 241
Cuts at: 2643 4825 136
Size: 2182 1525
Fragments arranged by size:
2182 1525 721 631 605 241 122 107
71 9
BglII A'GATC_T
Cuts at: 2691 2736 2952 6209 2691
Size: 45 216 3257 2696
Fragments arranged by size:
3257 2696 216 45
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2159 2378 3119 3294 4275 5431 1790
Size: 303 66 219 741 175 981 1156 2573
Fragments arranged by size:
2573 1156 981 741 303 219 175 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3945 4903 442
Size: 416 53 83 399 24 2528 958 1753
Fragments arranged by size:
2528 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2912 1056
Size: 1856 4358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2906 4874 1239
Size: 1094 573 1968 2579
Fragments arranged by size:
2579 1968 1094 573
Bpu10I CC'TnA_GC
Cuts at: 2566 2566
Size: 6214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4339 5204 5502 4339
Size: 865 298 5051
Fragments arranged by size:
5051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2061 4877 882
Size: 617 562 2816 2219
Fragments arranged by size:
2816 2219 617 562
BsaAI yAC'GTr
Cuts at: 493 1204 2109 2947 4005 493
Size: 711 905 838 1058 2702
Fragments arranged by size:
2702 1058 905 838 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2242 4021 4403 5038
Size: 53 83 186 936 709 1779 382 635
Cuts at: 5038 275
Size: 1451
Fragments arranged by size:
1779 1451 936 709 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2058 2088 2311 2341 2783
Size: 30 165 30 655 30 223 30 442
Cuts at: 2783 2813 3404 3434 1178
Size: 30 591 30 3958
Fragments arranged by size:
3958 655 591 442 223 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2689 1186
Size: 1006 497 4711
Fragments arranged by size:
4711 1006 497
BseYI C'CCAG_C
Cuts at: 2473 3302 5417 5913 2473
Size: 829 2115 496 2774
Fragments arranged by size:
2774 2115 829 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2447 2533 2606 2927 1163
Size: 573 353 358 86 73 321 4450
Fragments arranged by size:
4450 573 358 353 321 86 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2159 2232 2816 2850 4275 4360 5431 2159
Size: 73 584 34 1425 85 1071 2942
Fragments arranged by size:
2942 1425 1071 584 85 73 34
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
BsmBI CGTCTCn'nnnn_
Cuts at: 2088 2088
Size: 6214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2093 2120 2159 2232 2378 2574
Size: 399 228 75 27 39 73 146 196
Cuts at: 2574 2816 2850 2892 3119 3294 3533 4275 4360
Size: 242 34 42 227 175 239 742 85
Cuts at: 4360 5431 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 242 239 228 227
196 175 146 85 75 73 42 39
34 27
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 2147 2596 2949 3036 3569 844
Size: 1267 36 449 353 87 533 3489
Fragments arranged by size:
3489 1267 533 449 353 87 36
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 2979 3013 4709 4883 65
Size: 1183 874 857 34 1696 174 1396
Fragments arranged by size:
1696 1396 1183 874 857 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 3363 4858 5045 1700
Size: 110 1553 1495 187 2869
Fragments arranged by size:
2869 1553 1495 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 2843 4274 5286 2843
Size: 1431 1012 3771
Fragments arranged by size:
3771 1431 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3168 794
Size: 2374 3840
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 2056 2098 1184
Size: 872 42 5300
Fragments arranged by size:
5300 872 42
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2691 2736 2952 3156 3544 3911 4296 4313
Size: 719 45 216 204 388 367 385 17
Cuts at: 4313 5765 5851 5863 6209 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 719 388 385 367 346 216
204 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3808
Fragments arranged by size:
3808 1854 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2767 3025 487
Size: 683 56 1541 258 3676
Fragments arranged by size:
3676 1541 683 258 56
BtsI GCAGTG_nn'
Cuts at: 3038 3730 4524 4544 3038
Size: 692 794 20 4708
Fragments arranged by size:
4708 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2299 2299
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2353 2682 3661
Size: 54 1425 50 275 18 523 329 979
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 979 889 523 329 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4144 1353
Size: 2791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 2230
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2906 4277 4874 5661 5929 1239
Size: 393 701 573 1371 597 787 268 1524
Fragments arranged by size:
1524 1371 787 701 597 573 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2089 2132 2148 2375 2530 2089
Size: 43 16 227 155 5773
Fragments arranged by size:
5773 227 155 43 16
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2245 5361 1678
Size: 567 3116 2531
Fragments arranged by size:
3116 2531 567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 2783 2815 4855
Size: 32 135 32 1127 32 52 32 2040
Cuts at: 4855 4887 4929 4961 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2040 1127 135 52 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3558 3804 669
Size: 988 281 322 1298 246 3079
Fragments arranged by size:
3079 1298 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KasI G'GCGC_C
Cuts at: 2241 2241
Size: 6214
KpnI G_GTAC'C
Cuts at: 3424 3539 3424
Size: 115 6099
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 5328 5512 1305
Size: 1153 2870 184 2007
Fragments arranged by size:
2870 2007 1153 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2355 10
Size: 54 1425 343 523 3869
Fragments arranged by size:
3869 1425 523 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 4172 4531 4690 518
Size: 648 996 2010 359 159 2042
Fragments arranged by size:
2042 2010 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2359 2821 2921 3630 4307 5455 5700
Size: 489 264 462 100 709 677 1148 245
Cuts at: 5700 1606
Size: 2120
Fragments arranged by size:
2120 1148 709 677 489 462 264 245
100
NarI GG'CG_CC
Cuts at: 2242 2242
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5662
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 2908 1052
Size: 1856 4358
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2432 2524 2928 3572 5117 1132
Size: 97 1203 92 404 644 1545 2229
Fragments arranged by size:
2229 1545 1203 644 404 97 92
PasI CC'CwG_GG
Cuts at: 1348 2062 2407 1348
Size: 714 345 5155
Fragments arranged by size:
5155 714 345
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6214
PfoI T'CCnGG_A
Cuts at: 2535 2724 2535
Size: 189 6025
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 2109 2947 2109
Size: 838 5376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 2530
Size: 6214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 2089 2374 2089
Size: 285 5929
PspXI vC'TCGA_Gb
Cuts at: 2701 2809 2701
Size: 108 6106
PstI C_TGCA'G
Cuts at: 830 2237 2874 3033 3376 3566 830
Size: 1407 637 159 343 190 3478
Fragments arranged by size:
3478 1407 637 343 190 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2359 2821 2359
Size: 462 5752
SacI G_AGCT'C
Cuts at: 2232 2232
Size: 6214
SacII CC_GC'GG
Cuts at: 2922 2922
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 2233 2870 3029 3265 3372 3562 4697
Size: 219 1188 637 159 236 107 190 1135
Cuts at: 4697 5378 5569 826
Size: 681 191 1471
Fragments arranged by size:
1471 1188 1135 681 637 236 219 191
190 159 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 2393 2402 2393
Size: 9 6205
SfoI GGC'GCC
Cuts at: 2243 2243
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2701 2809 4318 5219 5481 820
Size: 197 926 758 108 1509 901 262 1553
Fragments arranged by size:
1553 1509 926 901 758 262 197 108
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 2928 3572 1132
Size: 1796 644 3774
Fragments arranged by size:
3774 1796 644
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 2031 3393 1517
Size: 514 1362 4338
Fragments arranged by size:
4338 1362 514
StyI C'CwwG_G
Cuts at: 513 1065 2010 2052 2613 2773 3653 513
Size: 552 945 42 561 160 880 3074
Fragments arranged by size:
3074 945 880 561 552 160 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 2399 4241 4258 4411 4596 5041
Size: 1255 756 63 1842 17 153 185 445
Cuts at: 5041 325
Size: 1498
Fragments arranged by size:
1842 1498 1255 756 445 185 153 63
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4460 6172 96
Size: 274 80 33 51 1443 2483 1712 138
Fragments arranged by size:
2483 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 2285 2311 2317 2343 6174 6206 2058
Size: 32 195 26 6 26 3831 32 2066
Fragments arranged by size:
3831 2066 195 32 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 1316 3380 1316
Size: 2064 4150
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3127 3127
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 2701 2809 1943
Size: 758 108 5348
Fragments arranged by size:
5348 758 108
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PsrI RsrII SanDI SapI SexAI
SrfI SwaI