(Linear) (Six-base) MAPSORT of: KIEE0962.seq Check: 9672 from: 1 to: 1509 KIEE0962.orf With 182 enzymes: SgfI * March 22, 2012 15:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 944 1509 Size: 944 565 Acc65I G'GTAC_C Cuts at: 0 1415 1509 Size: 1415 94 AflIII A'CryG_T Cuts at: 0 103 293 1509 Size: 103 190 1216 Fragments arranged by size: 1216 190 103 AleI CACnn'nnGTG Cuts at: 0 157 1509 Size: 157 1352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 175 1509 Size: 175 1334 AlwNI CAG_nnn'CTG Cuts at: 0 542 879 1509 Size: 542 337 630 Fragments arranged by size: 630 542 337 ApaI G_GGCC'C Cuts at: 0 88 373 1509 Size: 88 285 1136 Fragments arranged by size: 1136 285 88 ApaLI G'TGCA_C Cuts at: 0 150 1509 Size: 150 1359 AvaI C'yCGr_G Cuts at: 0 80 696 804 1101 1132 1509 Size: 80 616 108 297 31 377 Fragments arranged by size: 616 377 297 108 80 31 AvrII C'CTAG_G Cuts at: 0 768 1509 Size: 768 741 BanI G'GyrC_C Cuts at: 0 182 236 1111 1128 1284 1415 1509 Size: 182 54 875 17 156 131 94 Fragments arranged by size: 875 182 156 131 94 54 17 BanII G_rGCy'C Cuts at: 0 13 88 115 227 373 569 887 1509 Size: 13 75 27 112 146 196 318 622 Fragments arranged by size: 622 318 196 146 112 75 27 13 BbeI G_GCGC'C Cuts at: 0 240 1509 Size: 240 1269 BbsI GAAGACnn'nnnn_ Cuts at: 0 1465 1509 Size: 1465 44 BglI GCCn_nnn'nGGC Cuts at: 0 388 397 638 1509 Size: 388 9 241 871 Fragments arranged by size: 871 388 241 9 BglII A'GATC_T Cuts at: 0 686 731 947 1509 Size: 686 45 216 562 Fragments arranged by size: 686 562 216 45 Bme1580I G_kGCm'C Cuts at: 0 88 154 373 1114 1289 1509 Size: 88 66 219 741 175 220 Fragments arranged by size: 741 220 219 175 88 66 BmtI G_CTAG'C Cuts at: 0 907 1509 Size: 907 602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 864 1509 Size: 864 645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 901 1509 Size: 328 573 608 Fragments arranged by size: 608 573 328 Bpu10I CC'TnA_GC Cuts at: 0 561 1509 Size: 561 948 BsaI GGTCTCn'nnnn_ Cuts at: 0 56 1509 Size: 56 1453 BsaAI yAC'GTr Cuts at: 0 104 942 1509 Size: 104 838 567 Fragments arranged by size: 838 567 104 BsaHI Gr'CG_yC Cuts at: 0 237 1509 Size: 237 1272 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 53 83 306 336 778 808 1399 1429 Size: 53 30 223 30 442 30 591 30 Cuts at: 1429 1509 Size: 80 Fragments arranged by size: 591 442 223 80 53 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 187 684 1509 Size: 187 497 825 Fragments arranged by size: 825 497 187 BseYI C'CCAG_C Cuts at: 0 468 1297 1509 Size: 468 829 212 Fragments arranged by size: 829 468 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 442 528 601 922 1509 Size: 84 358 86 73 321 587 Fragments arranged by size: 587 358 321 86 84 73 BsiHKAI G_wGCw'C Cuts at: 0 154 227 811 845 1509 Size: 154 73 584 34 664 Fragments arranged by size: 664 584 154 73 34 BsmBI CGTCTCn'nnnn_ Cuts at: 0 83 1509 Size: 83 1426 Bsp1286I G_dGCh'C Cuts at: 0 13 88 115 154 227 373 569 811 Size: 13 75 27 39 73 146 196 242 Cuts at: 811 845 887 1114 1289 1509 Size: 34 42 227 175 220 Fragments arranged by size: 242 227 220 196 175 146 75 73 42 39 34 27 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 106 142 591 944 1031 1509 Size: 106 36 449 353 87 478 Fragments arranged by size: 478 449 353 106 87 36 BsrDI GCAATG_nn' Cuts at: 0 117 974 1008 1509 Size: 117 857 34 501 Fragments arranged by size: 857 501 117 34 BsrFI r'CCGG_y Cuts at: 0 1358 1509 Size: 1358 151 BssSI C'ACGA_G Cuts at: 0 838 1509 Size: 838 671 BstAPI GCAn_nnn'nTGC Cuts at: 0 1163 1509 Size: 1163 346 BstEII G'GTnAC_C Cuts at: 0 51 93 1509 Size: 51 42 1416 Fragments arranged by size: 1416 51 42 BstYI r'GATC_y Cuts at: 0 686 731 947 1151 1509 Size: 686 45 216 204 358 Fragments arranged by size: 686 358 216 204 45 BtgI C'CryG_G Cuts at: 0 914 1509 Size: 914 595 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 762 1020 1509 Size: 762 258 489 Fragments arranged by size: 762 489 258 BtsI GCAGTG_nn' Cuts at: 0 1033 1509 Size: 1033 476 DraIII CAC_nnn'GTG Cuts at: 0 294 1509 Size: 294 1215 EaeI y'GGCC_r Cuts at: 0 348 677 1509 Size: 348 329 832 Fragments arranged by size: 832 348 329 EcoICRI GAG'CTC Cuts at: 0 225 1509 Size: 225 1284 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 901 1509 Size: 328 573 608 Fragments arranged by size: 608 573 328 EcoO109I rG'GnC_Cy Cuts at: 0 84 127 143 370 525 1509 Size: 84 43 16 227 155 984 Fragments arranged by size: 984 227 155 84 43 16 HaeII r_GCGC'y Cuts at: 0 240 1509 Size: 240 1269 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 694 726 778 810 1509 Size: 694 32 52 32 699 Fragments arranged by size: 699 694 52 32 32 HincII GTy'rAC Cuts at: 0 255 1509 Size: 255 1254 KasI G'GCGC_C Cuts at: 0 236 1509 Size: 236 1273 KpnI G_GTAC'C Cuts at: 0 1419 1509 Size: 1419 90 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 453 1509 Size: 453 1056 MscI TGG'CCA Cuts at: 0 350 1509 Size: 350 1159 MslI CAynn'nnrTG Cuts at: 0 157 1509 Size: 157 1352 MspA1I CmG'CkG Cuts at: 0 90 354 816 916 1509 Size: 90 264 462 100 593 Fragments arranged by size: 593 462 264 100 90 NarI GG'CG_CC Cuts at: 0 237 1509 Size: 237 1272 NheI G'CTAG_C Cuts at: 0 903 1509 Size: 903 606 NspI r_CATG'y Cuts at: 0 427 519 923 1509 Size: 427 92 404 586 Fragments arranged by size: 586 427 404 92 PasI CC'CwG_GG Cuts at: 0 57 402 1509 Size: 57 345 1107 Fragments arranged by size: 1107 345 57 PfoI T'CCnGG_A Cuts at: 0 530 719 1509 Size: 530 189 790 Fragments arranged by size: 790 530 189 PmlI CAC'GTG Cuts at: 0 104 942 1509 Size: 104 838 567 Fragments arranged by size: 838 567 104 PpuMI rG'GwC_Cy Cuts at: 0 525 1509 Size: 525 984 PspOMI G'GGCC_C Cuts at: 0 84 369 1509 Size: 84 285 1140 Fragments arranged by size: 1140 285 84 PspXI vC'TCGA_Gb Cuts at: 0 696 804 1509 Size: 696 108 705 Fragments arranged by size: 705 696 108 PstI C_TGCA'G Cuts at: 0 232 869 1028 1371 1509 Size: 232 637 159 343 138 Fragments arranged by size: 637 343 232 159 138 PvuII CAG'CTG Cuts at: 0 354 816 1509 Size: 354 462 693 Fragments arranged by size: 693 462 354 SacI G_AGCT'C Cuts at: 0 227 1509 Size: 227 1282 SacII CC_GC'GG Cuts at: 0 917 1509 Size: 917 592 SfcI C'TryA_G Cuts at: 0 228 865 1024 1260 1367 1509 Size: 228 637 159 236 107 142 Fragments arranged by size: 637 236 228 159 142 107 SfiI GGCCn_nnn'nGGCC Cuts at: 0 388 397 1509 Size: 388 9 1112 Fragments arranged by size: 1112 388 9 SfoI GGC'GCC Cuts at: 0 238 1509 Size: 238 1271 SmlI C'TyrA_G Cuts at: 0 696 804 1509 Size: 696 108 705 Fragments arranged by size: 705 696 108 SphI G_CATG'C Cuts at: 0 923 1509 Size: 923 586 StuI AGG'CCT Cuts at: 0 26 1388 1509 Size: 26 1362 121 Fragments arranged by size: 1362 121 26 StyI C'CwwG_G Cuts at: 0 5 47 608 768 1509 Size: 5 42 561 160 741 Fragments arranged by size: 741 561 160 42 5 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 331 394 1509 Size: 331 63 1115 Fragments arranged by size: 1115 331 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 53 85 280 306 312 338 1509 Size: 53 32 195 26 6 26 1171 Fragments arranged by size: 1171 195 53 32 26 26 6 Tth111I GACn'n_nGTC Cuts at: 0 1375 1509 Size: 1375 134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1122 1509 Size: 1122 387 XhoI C'TCGA_G Cuts at: 0 696 804 1509 Size: 696 108 705 Fragments arranged by size: 705 696 108 Enzymes that do cut: AarI Acc65I AflIII AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI BglI BglII Bme1580I BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NheI NspI PasI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SfcI SfiI SfoI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XhoI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI XbaI XmaI XmnI ZraI