Restriction map for
pFN21AE3707
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 209 TCCGGA T^CCGGA
AcyI 854 GGCGCC G[GA]^CG[CT]C
AspHI 808 GAGCTC G[AT]GC[AT]^C
BanI 854 GGCGCC G^G[CT][GA]CC
BbeI 854 GGCGCC GGCGC^C
BcnI 441 CCGGG CC^[GC]GG
BglI 733 GCCATAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 311 CTCCAG CTGGAG(16/14)
BsaHI 854 GGCGCC G[GA]^CG[CT]C
BsaI 361 GAGACC GGTCTC(1/5)
BsaWI 209 TCCGGA [AT]^CCGG[AT]
BseAI 209 TCCGGA T^CCGGA
BsgI 250 GTGCAG GTGCAG(16/14)
BsiEI 819 CGACCG CG[GA][CT]^CG
BsiHKAI 808 GAGCTC G[AT]GC[AT]^C
BsmAI 361 GAGAC GTCTC(1/5)
BspEI 209 TCCGGA T^CCGGA
BsrDI 297 CATTGC GCAATG(2/0)
CfoI 855 GCGC GCG^C
DsaI 61 CCATGG C^C[GA][CT]GG
EarI 433 GAAGAG CTCTTC(1/4)
EcoT14I 61 CCATGG C^C[AT][AT]GG
FauI 834 GCGGG CCCGC(4/6)
HaeII 854 GGCGCC [GA]GCGC^[CT]
HgaI 861 GACGC GACGC(5/10)
HhaI 855 GCGC GCG^C
Hin1I 854 GGCGCC G[GA]^CG[CT]C
HinP1I 855 GCGC G^CGC
HinfI 229 GAGTC G^A[ACGT]TC
KasI 854 GGCGCC G^GCGCC
Ksp632I 433 GAAGAG CTCTTC(1/4)
MaeII 887 ACGT A^CGT
MaeIII 127 GTGAC ^GT[ACGT]AC
MroI 209 TCCGGA T^CCGGA
MseI 461 TTAA T^TAA
MspA1I 136 CAGCTG C[AC]G^C[GT]G
NarI 854 GGCGCC GG^CGCC
NciI 441 CCGGG CC^[GC]GG
NcoI 61 CCATGG C^CATGG
NheI 178 GCTAGC G^CTAGC
NlaIII 62 CATG CATG^
PleI 229 GAGTC GAGTC(4/5)
PstI 112 CTGCAG CTGCA^G
PvuII 136 CAGCTG CAG^CTG
SacI 808 GAGCTC GAGCT^C
SbfI 111 CCTGCAGG CCTGCA^GG
SfoI 854 GGCGCC GGC^GCC
SmlI 865 CTCAAG C^T[CT][GA]AG
Sse8387I 111 CCTGCAGG CCTGCA^GG
StyI 61 CCATGG C^C[AT][AT]GG
TaqI 563 TCGA T^CGA
Tru9I 461 TTAA T^TAA
Tsp45I 127 GTGAC ^GT[GC]AC
TthHB8I 563 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwNI 508 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
661 CAGAACCTG
AvaII 117 GGTCC G^G[AT]CC
529 GGTCC
BanII 688 GAGCCC G[GA]GC[CT]^C
808 GAGCTC
BbvI 182 GCAGC GCAGC(8/12)
252 GCAGC
BsmFI 39 GGGAC GGGAC(10/14)
231 GTCCC
BspMI 352 ACCTGC ACCTGC(4/8)
114 GCAGGT
BsrFI 557 GCCGGT [GA]^CCGG[CT]
606 GCCGGT
BsrI 378 CCAGT ACTGG(1/-1)
543 CCAGT
Cfr10I 557 GCCGGT [GA]^CCGG[CT]
606 GCCGGT
MboII 433 GAAGA GAAGA(8/7)
762 GAAGA
SfcI 112 CTGCAG C^T[GA][CT]AG
537 CTACAG
TseI 182 GCAGC G^C[AT]GC
252 GCAGC
Tsp509I 348 AATT ^AATT
798 AATT
TspRI 770 CACTG CA[GC]TG[ACGT][ACGT]^
792 CACTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MboI MflI MnlI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI XhoII