(Circular) (Six-base) MAPSORT of: pFN21AE3707.seq Check: 7167 from: 1 to: 5608 pFN21AE3707 5608 bp With 182 enzymes: SgfI * March 28, 2012 12:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3671 5055 5323 1632 Size: 2039 1384 268 1917 Fragments arranged by size: 2039 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2210 1184 Size: 1026 4582 AlwNI CAG_nnn'CTG Cuts at: 2518 2671 3351 4923 2518 Size: 153 680 1572 3203 Fragments arranged by size: 3203 1572 680 153 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2859 2929 4384 618 Size: 325 217 627 1072 70 1455 1842 Fragments arranged by size: 1842 1455 1072 627 325 217 70 BanII G_rGCy'C Cuts at: 1391 2697 2817 2927 1391 Size: 1306 120 110 4072 Fragments arranged by size: 4072 1306 120 110 BbeI G_GCGC'C Cuts at: 2863 2863 Size: 5608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3799 3833 1903 Size: 34 50 34 1778 34 3678 Fragments arranged by size: 3678 1778 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2744 4219 136 Size: 107 122 71 631 605 1072 1475 1525 Fragments arranged by size: 1525 1475 1072 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2286 2599 3669 4825 1790 Size: 496 313 1070 1156 2573 Fragments arranged by size: 2573 1156 1070 496 313 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 4297 442 Size: 416 53 83 399 24 1922 958 1753 Fragments arranged by size: 1922 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2187 1056 Size: 1131 4477 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 4268 1239 Size: 1062 1967 2579 Fragments arranged by size: 2579 1967 1062 Bpu10I CC'TnA_GC Cuts at: 2040 2808 2040 Size: 768 4840 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2855 3733 4598 4896 2855 Size: 878 865 298 3567 Fragments arranged by size: 3567 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2360 4271 882 Size: 617 861 1911 2219 Fragments arranged by size: 2219 1911 861 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2860 3415 3797 4432 Size: 53 83 186 936 1327 555 382 635 Cuts at: 4432 275 Size: 1451 Fragments arranged by size: 1451 1327 936 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 4041 4439 4713 4860 5271 2214 Size: 1827 398 274 147 411 2551 Fragments arranged by size: 2551 1827 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5608 BseYI C'CCAG_C Cuts at: 2098 2139 2286 2311 2485 4811 5307 2098 Size: 41 147 25 174 2326 496 2399 Fragments arranged by size: 2399 2326 496 174 147 41 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 1163 Size: 573 540 4495 Fragments arranged by size: 4495 573 540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3058 3819 4847 1540 Size: 275 186 826 231 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 2817 3669 3754 4825 2817 Size: 852 85 1071 3600 Fragments arranged by size: 3600 1071 852 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2599 2697 2817 2927 3669 3754 Size: 399 496 313 98 120 110 742 85 Cuts at: 3754 4825 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 496 399 313 120 110 98 85 BspEI T'CCGG_A Cuts at: 2214 5271 2214 Size: 3057 2551 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2110 2366 2963 844 Size: 1266 256 597 3489 Fragments arranged by size: 3489 1266 597 256 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2301 4103 4277 65 Size: 1183 1053 1802 174 1396 Fragments arranged by size: 1802 1396 1183 1053 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 2611 4252 4439 1700 Size: 110 752 49 1641 187 2869 Fragments arranged by size: 2869 1641 752 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2025 2442 2469 2901 2938 3305 3690 3707 Size: 53 417 27 432 37 367 385 17 Cuts at: 3707 5159 5245 5257 5603 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 432 417 385 367 346 86 53 37 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2066 513 Size: 552 1001 4055 Fragments arranged by size: 4055 1001 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2774 3124 3918 3938 2774 Size: 350 794 20 4444 Fragments arranged by size: 4444 794 350 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573 Size: 54 1425 50 275 18 1225 889 1629 Cuts at: 5573 8 Size: 43 Fragments arranged by size: 1629 1425 1225 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 3538 1353 Size: 1080 1105 3423 Fragments arranged by size: 3423 1105 1080 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2815 2815 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 3671 4268 5055 5323 1239 Size: 393 669 1370 597 787 268 1524 Fragments arranged by size: 1524 1370 787 669 597 393 268 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2863 4755 1678 Size: 1185 1892 2531 Fragments arranged by size: 2531 1892 1185 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2005 2037 4249 4281 4323 Size: 32 135 32 433 32 2212 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 2212 433 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KasI G'GCGC_C Cuts at: 2859 2859 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 3024 3701 4849 5094 1606 Size: 537 881 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 677 537 245 NarI GG'CG_CC Cuts at: 2860 2860 Size: 5608 NcoI C'CATG_G Cuts at: 513 1065 2066 513 Size: 552 1001 4055 Fragments arranged by size: 4055 1001 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 2183 1052 Size: 1131 4477 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 2158 1348 Size: 810 4798 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PstI C_TGCA'G Cuts at: 830 2121 2960 830 Size: 1291 839 3478 Fragments arranged by size: 3478 1291 839 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2143 2143 Size: 5608 SacI G_AGCT'C Cuts at: 2817 2817 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2121 2960 2121 Size: 839 4769 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2117 2542 2956 4091 4772 4963 826 Size: 219 1072 425 414 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 425 414 219 191 SfoI GGC'GCC Cuts at: 2861 2861 Size: 5608 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 3712 4613 4875 820 Size: 197 926 927 842 901 262 1553 Fragments arranged by size: 1553 927 926 901 842 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2066 3047 513 Size: 552 1001 981 3074 Fragments arranged by size: 3074 1001 981 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2602 3635 3652 3805 3990 4435 325 Size: 1255 1022 1033 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1033 1022 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI