Restriction map for
pFN21AE3707
(Linear) (Six-base) MAPSORT of: KIEE3707.seq Check: 3473 from: 1 to: 903
KIEE3707.orf
With 182 enzymes: SgfI *
March 22, 2012 16:40 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 903
Size: 205 698
AlwNI CAG_nnn'CTG
Cuts at: 0 513 666 903
Size: 513 153 237
Fragments arranged by size:
513 237 153
BanI G'GyrC_C
Cuts at: 0 854 903
Size: 854 49
BanII G_rGCy'C
Cuts at: 0 692 812 903
Size: 692 120 91
Fragments arranged by size:
692 120 91
BbeI G_GCGC'C
Cuts at: 0 858 903
Size: 858 45
BglI GCCn_nnn'nGGC
Cuts at: 0 739 903
Size: 739 164
Bme1580I G_kGCm'C
Cuts at: 0 281 594 903
Size: 281 313 309
Fragments arranged by size:
313 309 281
BmtI G_CTAG'C
Cuts at: 0 182 903
Size: 182 721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 903
Size: 296 607
Bpu10I CC'TnA_GC
Cuts at: 0 35 803 903
Size: 35 768 100
Fragments arranged by size:
768 100 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 903
Size: 850 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 903
Size: 355 548
BsaHI Gr'CG_yC
Cuts at: 0 855 903
Size: 855 48
BsaWI w'CCGG_w
Cuts at: 0 209 903
Size: 209 694
BseYI C'CCAG_C
Cuts at: 0 93 134 281 306 480 903
Size: 93 41 147 25 174 423
Fragments arranged by size:
423 174 147 93 41 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 903
Size: 271 632
BsiEI CG_ry'CG
Cuts at: 0 822 903
Size: 822 81
BsiHKAI G_wGCw'C
Cuts at: 0 812 903
Size: 812 91
Bsp1286I G_dGCh'C
Cuts at: 0 281 594 692 812 903
Size: 281 313 98 120 91
Fragments arranged by size:
313 281 120 98 91
BspEI T'CCGG_A
Cuts at: 0 209 903
Size: 209 694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 361 903
Size: 105 256 542
Fragments arranged by size:
542 256 105
BsrDI GCAATG_nn'
Cuts at: 0 296 903
Size: 296 607
BsrFI r'CCGG_y
Cuts at: 0 557 606 903
Size: 557 49 297
Fragments arranged by size:
557 297 49
BstYI r'GATC_y
Cuts at: 0 20 437 464 896 903
Size: 20 417 27 432 7
Fragments arranged by size:
432 417 27 20 7
BtgI C'CryG_G
Cuts at: 0 61 903
Size: 61 842
BtsI GCAGTG_nn'
Cuts at: 0 769 903
Size: 769 134
EarI CTCTTCn'nnn_
Cuts at: 0 428 903
Size: 428 475
EcoICRI GAG'CTC
Cuts at: 0 810 903
Size: 810 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 903
Size: 296 607
HaeII r_GCGC'y
Cuts at: 0 858 903
Size: 858 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 903
Size: 32 871
KasI G'GCGC_C
Cuts at: 0 854 903
Size: 854 49
MspA1I CmG'CkG
Cuts at: 0 138 903
Size: 138 765
NarI GG'CG_CC
Cuts at: 0 855 903
Size: 855 48
NcoI C'CATG_G
Cuts at: 0 61 903
Size: 61 842
NheI G'CTAG_C
Cuts at: 0 178 903
Size: 178 725
PasI CC'CwG_GG
Cuts at: 0 153 903
Size: 153 750
PfoI T'CCnGG_A
Cuts at: 0 440 903
Size: 440 463
PstI C_TGCA'G
Cuts at: 0 116 903
Size: 116 787
PvuII CAG'CTG
Cuts at: 0 138 903
Size: 138 765
SacI G_AGCT'C
Cuts at: 0 812 903
Size: 812 91
SbfI CC_TGCA'GG
Cuts at: 0 116 903
Size: 116 787
SfcI C'TryA_G
Cuts at: 0 112 537 903
Size: 112 425 366
Fragments arranged by size:
425 366 112
SfoI GGC'GCC
Cuts at: 0 856 903
Size: 856 47
SmlI C'TyrA_G
Cuts at: 0 865 903
Size: 865 38
StyI C'CwwG_G
Cuts at: 0 61 903
Size: 61 842
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 903
Size: 597 306
Enzymes that do cut:
AlfI AlwNI BanI BanII BbeI BglI Bme1580I BmtI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstYI
BtgI BtsI EarI EcoICRI Eco57MI HaeII Hin4I KasI
MspA1I NarI NcoI NheI PasI PfoI PstI PvuII
SacI SbfI SfcI SfoI SmlI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BplI BsaAI BsaBI BsaXI BseRI BsiWI BsmI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NdeI
NgoMIV NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI