Restriction map for
pFN21AE1788
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AocI
Aor51HI AscI AseI AsnI Asp718I
AspI AvaI AvaII AviII AvrII
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HapII HgaI HindIII HpaI
HpaII KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MspA1I
MspI MunI NaeI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 22 CGCG CG^CG
AcsI 217 AAATTT [GA]^AATT[CT]
AcyI 581 GGCGCC G[GA]^CG[CT]C
AhdI 704 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 566 GTGCAC G^TGCAC
AlwNI 247 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 471 GGGCCC GGGCC^C
ApaLI 566 GTGCAC G^TGCAC
ApoI 217 AAATTT [GA]^AATT[CT]
Asp700I 654 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 704 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 566 GTGCAC G[AT]GC[AT]^C
BalI 848 TGGCCA TGG^CCA
BamHI 667 GGATCC G^GATCC
BanI 581 GGCGCC G^G[CT][GA]CC
BbeI 581 GGCGCC GGCGC^C
BpmI 481 CTGGAG CTGGAG(16/14)
BsaHI 581 GGCGCC G[GA]^CG[CT]C
BsgI 156 CTGCAC GTGCAG(16/14)
BsiHKAI 566 GTGCAC G[AT]GC[AT]^C
BsmAI 149 GAGAC GTCTC(1/5)
BsmI 448 GCATTC GAATGC(1/-1)
BspHI 836 TCATGA T^CATGA
BspMI 550 ACCTGC ACCTGC(4/8)
BsrBI 113 CCGCTC CCGCTC(-3/-3)
BsrDI 538 GCAATG GCAATG(2/0)
BstEII 129 GGTTACC G^GT[ACGT]ACC
BstF5I 510 CATCC GGATG(2/0)
BstPI 129 GGTTACC G^GT[ACGT]ACC
BstUI 22 CGCG CG^CG
BstYI 667 GGATCC [GA]^GATC[CT]
DraI 213 TTTAAA TTT^AAA
DsaI 100 CCACGG C^C[GA][CT]GG
EaeI 848 TGGCCA [CT]^GGCC[GA]
Eam1105I 704 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 802 CTCTTC CTCTTC(1/4)
EcoNI 252 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 129 GGTTACC G^GT[ACGT]ACC
EcoT14I 356 CCTTGG C^C[AT][AT]GG
FokI 510 CATCC GGATG(9/13)
HaeII 581 GGCGCC [GA]GCGC^[CT]
Hin1I 581 GGCGCC G[GA]^CG[CT]C
HphI 557 TCACC GGTGA(8/7)
KasI 581 GGCGCC G^GCGCC
Ksp632I 802 CTCTTC CTCTTC(1/4)
MflI 667 GGATCC [GA]^GATC[CT]
MluNI 848 TGGCCA TGG^CCA
MscI 848 TGGCCA TGG^CCA
MseI 214 TTAA T^TAA
MvnI 22 CGCG CG^CG
NarI 581 GGCGCC GG^CGCC
NdeI 316 CATATG CA^TATG
PstI 299 CTGCAG CTGCA^G
RcaI 836 TCATGA T^CATGA
SfcI 299 CTGCAG C^T[GA][CT]AG
SfoI 581 GGCGCC GGC^GCC
StyI 356 CCTTGG C^C[AT][AT]GG
TaqI 189 TCGA T^CGA
Tru9I 214 TTAA T^TAA
Tsp509I 218 AATT ^AATT
TthHB8I 189 TCGA T^CGA
XcmI 518 CCACTGCTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 667 GGATCC [GA]^GATC[CT]
XmnI 654 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 589 AGCT AG^CT
628 AGCT
BanII 360 GGGCTC G[GA]GC[CT]^C
471 GGGCCC
BbsI 642 GTCTTC GAAGAC(2/6)
823 GTCTTC
BglI 89 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
98 GCCCACGGGGC
BpuAI 642 GTCTTC GAAGAC(2/6)
823 GTCTTC
BsmFI 429 GGGAC GGGAC(10/14)
702 GGGAC
BsrI 426 ACTGG ACTGG(1/-1)
705 ACTGG
BstAPI 268 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
568 GCACTCTATGC
Cac8I 340 GCTGGC GC[ACGT]^[ACGT]GC
344 GCCTGC
CfoI 208 GCGC GCG^C
582 GCGC
DraII 304 GGGGCCT [GA]G^G[ACGT]CC[CT]
471 GGGCCCT
EcoO109I 304 GGGGCCT [GA]G^G[ACGT]CC[CT]
471 GGGCCCT
FauI 112 CCCGC CCCGC(4/6)
57 GCGGG
HhaI 208 GCGC GCG^C
582 GCGC
HinP1I 208 GCGC G^CGC
582 GCGC
HincII 237 GTCAAC GT[CT]^[GA]AC
499 GTCAAC
HindII 237 GTCAAC GT[CT]^[GA]AC
499 GTCAAC
HinfI 71 GAGTC G^A[ACGT]TC
163 GACTC
MslI 400 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
534 CACGGCAATG
PleI 163 GACTC GAGTC(4/5)
71 GAGTC
SfaNI 205 GATGC GCATC(5/9)
509 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AlwI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI