(Circular) (Six-base) MAPSORT of: pFN21AE1788.seq Check: 6125 from: 1 to: 5590 pFN21AE1788 5590 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2815 3653 5037 5305 1632 Size: 647 536 838 1384 268 1917 Fragments arranged by size: 1917 1384 838 647 536 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 2714 4319 2714 Size: 1605 3985 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 2257 3333 4905 2257 Size: 1076 1572 2942 Fragments arranged by size: 2942 1572 1076 ApaI G_GGCC'C Cuts at: 2480 2480 Size: 5590 ApaLI G'TGCA_C Cuts at: 2571 3647 4803 2571 Size: 1076 1156 3358 Fragments arranged by size: 3358 1156 1076 ApoI r'AATT_y Cuts at: 1493 2222 2899 3125 3277 5386 1493 Size: 729 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 729 677 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2672 2920 3287 2672 Size: 248 367 4975 Fragments arranged by size: 4975 367 248 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2586 2911 4366 618 Size: 325 217 627 799 325 1455 1842 Fragments arranged by size: 1842 1455 799 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 2369 2480 2909 1391 Size: 978 111 429 4072 Fragments arranged by size: 4072 978 429 111 BbeI G_GCGC'C Cuts at: 2590 2590 Size: 5590 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 2821 928 Size: 971 741 181 3697 Fragments arranged by size: 3697 971 741 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 2109 4201 Size: 107 122 71 631 605 428 9 2092 Cuts at: 4201 136 Size: 1525 Fragments arranged by size: 2092 1525 631 605 428 122 107 71 9 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2480 2540 2575 3651 4807 1790 Size: 690 60 35 1076 1156 2573 Fragments arranged by size: 2573 1156 1076 690 60 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 3321 4279 Size: 416 53 83 399 24 1024 880 958 Cuts at: 4279 442 Size: 1753 Fragments arranged by size: 1753 1024 958 880 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 4250 1239 Size: 1268 1743 2579 Fragments arranged by size: 2579 1743 1268 Bpu10I CC'TnA_GC Cuts at: 2630 2630 Size: 5590 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2587 3397 3779 4414 Size: 53 83 186 936 1054 810 382 635 Cuts at: 4414 275 Size: 1451 Fragments arranged by size: 1451 1054 936 810 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2005 2025 2088 1186 Size: 819 20 63 4688 Fragments arranged by size: 4688 819 63 20 BseYI C'CCAG_C Cuts at: 2085 2510 4793 5289 2085 Size: 425 2283 496 2386 Fragments arranged by size: 2386 2283 496 425 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 1163 Size: 573 410 4607 Fragments arranged by size: 4607 573 410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2575 3651 3736 4807 2575 Size: 1076 85 1071 3358 Fragments arranged by size: 3358 1076 1071 85 BsmI GAATG_Cn' Cuts at: 2453 3101 3194 2453 Size: 648 93 4849 Fragments arranged by size: 4849 648 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2480 2540 2575 2909 3651 3736 Size: 399 579 111 60 35 334 742 85 Cuts at: 3736 4807 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 579 399 334 111 85 60 35 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 2841 3479 5209 2841 Size: 638 1730 3222 Fragments arranged by size: 3222 1730 638 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2564 2945 844 Size: 1720 381 3489 Fragments arranged by size: 3489 1720 381 BsrBI CCG'CTC Cuts at: 1394 1823 2120 3477 1394 Size: 429 297 1357 3507 Fragments arranged by size: 3507 1357 429 297 BsrDI GCAATG_nn' Cuts at: 65 1248 2550 4085 4259 65 Size: 1183 1302 1535 174 1396 Fragments arranged by size: 1535 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 2279 2579 794 Size: 1485 300 3805 Fragments arranged by size: 3805 1485 300 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 2134 1184 Size: 950 4640 BstYI r'GATC_y Cuts at: 1972 2672 2920 3287 3672 3689 5141 5227 5239 Size: 700 248 367 385 17 1452 86 12 Cuts at: 5239 5585 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 700 385 367 346 248 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2105 513 Size: 552 1040 3998 Fragments arranged by size: 3998 1040 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2523 2794 3106 3900 3920 2523 Size: 271 312 794 20 4193 Fragments arranged by size: 4193 794 312 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2220 2894 2220 Size: 674 4916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2853 3037 3926 Size: 54 1425 50 275 18 1023 184 889 Cuts at: 3926 5555 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1023 889 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2813 3520 1353 Size: 1460 707 3423 Fragments arranged by size: 3423 1460 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2507 2815 3653 4250 5037 5305 Size: 393 647 228 308 838 597 787 268 Cuts at: 5305 1239 Size: 1524 Fragments arranged by size: 1524 838 787 647 597 393 308 268 228 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2261 Size: 5590 EcoO109I rG'GnC_Cy Cuts at: 2310 2477 2310 Size: 167 5423 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2590 4737 1678 Size: 912 2147 2531 Fragments arranged by size: 2531 2147 912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2700 2732 4231 4263 4305 Size: 32 135 32 1128 32 1499 32 42 Cuts at: 4305 4337 1373 Size: 32 2626 Fragments arranged by size: 2626 1499 1128 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2244 2506 2934 3180 669 Size: 988 281 306 262 428 246 3079 Fragments arranged by size: 3079 988 428 306 281 262 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KasI G'GCGC_C Cuts at: 2586 2586 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2855 10 Size: 54 1425 343 1023 2745 Fragments arranged by size: 2745 1425 1023 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2409 2543 3548 3907 4066 518 Size: 648 1243 134 1005 359 159 2042 Fragments arranged by size: 2042 1243 1005 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 3006 3683 4831 5076 1606 Size: 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 245 NarI GG'CG_CC Cuts at: 2587 2587 Size: 5590 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5038 NdeI CA'TA_TG Cuts at: 387 2322 387 Size: 1935 3655 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 2653 1348 Size: 1305 4285 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2476 2476 Size: 5590 PstI C_TGCA'G Cuts at: 830 2308 2942 830 Size: 1478 634 3478 Fragments arranged by size: 3478 1478 634 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2304 2938 4073 4754 4945 826 Size: 219 1259 634 1135 681 191 1471 Fragments arranged by size: 1471 1259 1135 681 634 219 191 SfoI GGC'GCC Cuts at: 2588 2588 Size: 5590 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3694 4595 4857 820 Size: 197 926 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2361 3029 513 Size: 552 1296 668 3074 Fragments arranged by size: 3074 1296 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 2663 3719 2663 Size: 1056 4534 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI