Restriction map for
pFN21AB9895
(Linear) (Six-base) MAPSORT of: KIBB9895.seq Check: 6874 from: 1 to: 711
KIBB9895.orf
With 182 enzymes: SgfI *
February 12, 2011 08:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 636 711
Size: 100 536 75
Fragments arranged by size:
536 100 75
AhdI GACnn_n'nnGTC
Cuts at: 0 535 711
Size: 535 176
AlwNI CAG_nnn'CTG
Cuts at: 0 78 711
Size: 78 633
ApaI G_GGCC'C
Cuts at: 0 301 711
Size: 301 410
ApaLI G'TGCA_C
Cuts at: 0 392 711
Size: 392 319
ApoI r'AATT_y
Cuts at: 0 43 711
Size: 43 668
BamHI G'GATC_C
Cuts at: 0 493 711
Size: 493 218
BanI G'GyrC_C
Cuts at: 0 407 711
Size: 407 304
BanII G_rGCy'C
Cuts at: 0 190 301 711
Size: 190 111 410
Fragments arranged by size:
410 190 111
BbeI G_GCGC'C
Cuts at: 0 411 711
Size: 411 300
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 642 711
Size: 461 181 69
Fragments arranged by size:
461 181 69
Bme1580I G_kGCm'C
Cuts at: 0 301 361 396 711
Size: 301 60 35 315
Fragments arranged by size:
315 301 60 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 711
Size: 262 449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 711
Size: 328 383
Bpu10I CC'TnA_GC
Cuts at: 0 451 711
Size: 451 260
BsaHI Gr'CG_yC
Cuts at: 0 408 711
Size: 408 303
BseYI C'CCAG_C
Cuts at: 0 331 711
Size: 331 380
BsiHKAI G_wGCw'C
Cuts at: 0 396 711
Size: 396 315
BsmI GAATG_Cn'
Cuts at: 0 274 711
Size: 274 437
Bsp1286I G_dGCh'C
Cuts at: 0 190 301 361 396 711
Size: 190 111 60 35 315
Fragments arranged by size:
315 190 111 60 35
BspHI T'CATG_A
Cuts at: 0 662 711
Size: 662 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 385 711
Size: 385 326
BsrDI GCAATG_nn'
Cuts at: 0 371 711
Size: 371 340
BstAPI GCAn_nnn'nTGC
Cuts at: 0 100 400 711
Size: 100 300 311
Fragments arranged by size:
311 300 100
BstYI r'GATC_y
Cuts at: 0 493 711
Size: 493 218
BtsI GCAGTG_nn'
Cuts at: 0 344 615 711
Size: 344 271 96
Fragments arranged by size:
344 271 96
DraI TTT'AAA
Cuts at: 0 41 711
Size: 41 670
EaeI y'GGCC_r
Cuts at: 0 674 711
Size: 674 37
EarI CTCTTCn'nnn_
Cuts at: 0 634 711
Size: 634 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 328 636 711
Size: 100 228 308 75
Fragments arranged by size:
308 228 100 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 711
Size: 82 629
EcoO109I rG'GnC_Cy
Cuts at: 0 131 298 711
Size: 131 167 413
Fragments arranged by size:
413 167 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 711
Size: 42 669
HaeII r_GCGC'y
Cuts at: 0 411 711
Size: 411 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 711
Size: 521 32 158
Fragments arranged by size:
521 158 32
HincII GTy'rAC
Cuts at: 0 65 327 711
Size: 65 262 384
Fragments arranged by size:
384 262 65
KasI G'GCGC_C
Cuts at: 0 407 711
Size: 407 304
MscI TGG'CCA
Cuts at: 0 676 711
Size: 676 35
MslI CAynn'nnrTG
Cuts at: 0 230 364 711
Size: 230 134 347
Fragments arranged by size:
347 230 134
NarI GG'CG_CC
Cuts at: 0 408 711
Size: 408 303
NdeI CA'TA_TG
Cuts at: 0 143 711
Size: 143 568
PasI CC'CwG_GG
Cuts at: 0 474 711
Size: 474 237
PspOMI G'GGCC_C
Cuts at: 0 297 711
Size: 297 414
PstI C_TGCA'G
Cuts at: 0 129 711
Size: 129 582
SfcI C'TryA_G
Cuts at: 0 125 711
Size: 125 586
SfoI GGC'GCC
Cuts at: 0 409 711
Size: 409 302
StyI C'CwwG_G
Cuts at: 0 182 711
Size: 182 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 711
Size: 351 360
XmnI GAAnn'nnTTC
Cuts at: 0 484 711
Size: 484 227
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApaLI ApoI BamHI BanI
BanII BbeI BbsI Bme1580I BmrI BpmI Bpu10I BsaHI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI
BstYI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MscI MslI NarI
NdeI PasI PspOMI PstI SfcI SfoI StyI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI MspA1I
NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI ZraI