################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AvrII BalI BamHI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstPI BstYI Bsu36I Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FseI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 825 GTATAC GT^[AC][GT]AC AcsI 849 GAATTC [GA]^AATT[CT] AcyI 1059 GGCGCC G[GA]^CG[CT]C AlwNI 323 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 849 GAATTC [GA]^AATT[CT] AvaI 693 CTCGGG C^[CT]CG[GA]G AviII 1036 TGCGCA TGC^GCA BanI 1059 GGCGCC G^G[CT][GA]CC BbeI 1059 GGCGCC GGCGC^C BbvCI 922 CCTCAGC CCTCAGC(-5/-2) BclI 143 TGATCA T^GATCA BfaI 484 CTAG C^TAG BlpI 999 GCTGAGC GC^T[ACGT]AGC Bpu1102I 999 GCTGAGC GC^T[ACGT]AGC BsaHI 1059 GGCGCC G[GA]^CG[CT]C BsaI 954 GAGACC GGTCTC(1/5) BsaWI 1008 TCCGGT [AT]^CCGG[AT] BsmI 1004 GCATTC GAATGC(1/-1) BsoBI 693 CTCGGG C^[CT]CG[GA]G BspMI 718 ACCTGC ACCTGC(4/8) BsrI 450 ACTGG ACTGG(1/-1) BssHII 1053 GCGCGC G^CGCGC BssSI 139 CTCGTG CACGAG(-5/-1) Bst1107I 825 GTATAC GTA^TAC BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 825 GTATAC GTA^TAC CelII 999 GCTGAGC GC^T[ACGT]AGC DraII 1030 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 427 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1064 CCACGG C^C[GA][CT]GG EcoNI 291 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1030 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 849 GAATTC G^AATTC EcoRV 571 GATATC GAT^ATC FbaI 143 TGATCA T^GATCA FspI 1036 TGCGCA TGC^GCA HaeII 1059 GGCGCC [GA]GCGC^[CT] HgaI 463 GACGC GACGC(5/10) Hin1I 1059 GGCGCC G[GA]^CG[CT]C KasI 1059 GGCGCC G^GCGCC MaeI 484 CTAG C^TAG NarI 1059 GGCGCC GG^CGCC NspI 1039 GCATGC [GA]CATG^[CT] PleI 189 GAGTC GAGTC(4/5) PshAI 907 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 1059 GGCGCC GGC^GCC SphI 1039 GCATGC GCATG^C StuI 707 AGGCCT AGG^CCT Tsp509I 850 AATT ^AATT ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbsI 301 GTCTTC GAAGAC(2/6) 640 GTCTTC BpmI 576 CTCCAG CTGGAG(16/14) 244 CTGGAG BpuAI 301 GTCTTC GAAGAC(2/6) 640 GTCTTC BseRI 37 CTCCTC GAGGAG(10/8) 550 CTCCTC BsgI 928 CTGCAC GTGCAG(16/14) 820 GTGCAG BsmAI 954 GAGAC GTCTC(1/5) 161 GTCTC Cfr13I 1031 GGGCC G^G[ACGT]CC 1068 GGCCC EarI 219 CTCTTC CTCTTC(1/4) 951 GAAGAG Ksp632I 219 CTCTTC CTCTTC(1/4) 951 GAAGAG MslI 735 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 837 CACTCCCATG MspA1I 415 CAGCTG C[AC]G^C[GT]G 925 CAGCTG PstI 320 CTGCAG CTGCA^G 737 CTGCAG PvuII 415 CAGCTG CAG^CTG 925 CAGCTG Sau96I 1031 GGGCC G^G[ACGT]CC 1068 GGCCC TfiI 12 GAATC G^A[AT]TC 154 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI