(Circular) (Six-base) MAPSORT of: pFN21AE5079.seq Check: 3211 from: 1 to: 5782 pFN21AE5079 5782 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5782 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 3125 1937 Size: 894 294 4594 Fragments arranged by size: 4594 894 294 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 2355 3845 5229 5497 1632 Size: 579 144 1490 1384 268 1917 Fragments arranged by size: 1917 1490 1384 579 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2939 1184 Size: 1755 4027 AlwNI CAG_nnn'CTG Cuts at: 2333 3525 5097 2333 Size: 1192 1572 3018 Fragments arranged by size: 3018 1572 1192 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2854 3091 3317 3469 5578 1493 Size: 1361 237 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2698 3107 1870 Size: 73 755 409 4545 Fragments arranged by size: 4545 755 409 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 3103 4558 618 Size: 325 217 627 1277 39 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 39 BanII G_rGCy'C Cuts at: 1391 2080 2447 2991 3101 1391 Size: 689 367 544 110 4072 Fragments arranged by size: 4072 689 544 367 110 BbeI G_GCGC'C Cuts at: 3068 3068 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 2638 928 Size: 971 400 339 4072 Fragments arranged by size: 4072 971 400 339 BbvCI CC'TCA_GC Cuts at: 2928 2928 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2148 1548 Size: 600 5182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3005 3199 3005 Size: 194 5588 Bme1580I G_kGCm'C Cuts at: 1790 2241 3843 4999 1790 Size: 451 1602 1156 2573 Fragments arranged by size: 2573 1602 1156 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 3513 4471 Size: 416 53 83 399 24 1048 1048 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1048 1048 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2566 4442 1239 Size: 1031 296 1876 2579 Fragments arranged by size: 2579 1876 1031 296 Bpu10I CC'TnA_GC Cuts at: 2301 2681 2764 2928 2301 Size: 380 83 164 5155 Fragments arranged by size: 5155 380 164 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2953 4445 882 Size: 617 1454 1492 2219 Fragments arranged by size: 2219 1492 1454 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3065 3589 3971 4606 Size: 53 83 186 936 1532 524 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1532 1451 936 635 524 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3013 4215 4613 4887 5034 5445 3013 Size: 1202 398 274 147 411 3350 Fragments arranged by size: 3350 1202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2546 1186 Size: 847 513 4422 Fragments arranged by size: 4422 847 513 BseYI C'CCAG_C Cuts at: 2626 4985 5481 2626 Size: 2359 496 2927 Fragments arranged by size: 2927 2359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2846 2918 1163 Size: 573 1110 72 4027 Fragments arranged by size: 4027 1110 573 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3009 3293 3386 3009 Size: 284 93 5405 Fragments arranged by size: 5405 284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2241 2447 2991 3101 3843 3928 Size: 399 290 161 206 544 110 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 544 399 290 206 161 110 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 3137 844 Size: 1888 405 3489 Fragments arranged by size: 3489 1888 405 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 3058 1925 Size: 1133 4649 BssSI C'ACGA_G Cuts at: 2144 3842 4854 2144 Size: 1698 1012 3072 Fragments arranged by size: 3072 1698 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2248 2011 Size: 237 5545 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2832 2832 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 2004 3069 513 Size: 552 939 1065 3226 Fragments arranged by size: 3226 1065 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2578 487 Size: 683 56 1352 3691 Fragments arranged by size: 3691 1352 683 56 BtsI GCAGTG_nn' Cuts at: 2322 2739 2751 3298 4092 4112 2322 Size: 417 12 547 794 20 3992 Fragments arranged by size: 3992 794 547 417 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DraIII CAC_nnn'GTG Cuts at: 2437 2437 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 2951 3712 1353 Size: 877 721 761 3423 Fragments arranged by size: 3423 877 761 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 4369 4743 4889 387 Size: 1064 138 1176 1604 374 146 1280 Fragments arranged by size: 1604 1280 1176 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2270 2355 2566 3845 4442 5229 Size: 393 579 59 85 211 1279 597 787 Cuts at: 5229 5497 1239 Size: 268 1524 Fragments arranged by size: 1524 1279 787 597 579 393 268 211 85 59 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2300 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 3036 3036 Size: 5782 EcoRI G'AATT_C Cuts at: 2854 3091 3469 2854 Size: 237 378 5167 Fragments arranged by size: 5167 378 237 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2360 2360 Size: 5782 FspI TGC'GCA Cuts at: 3043 4288 3043 Size: 1245 4537 HaeII r_GCGC'y Cuts at: 1678 3068 4929 1678 Size: 1390 1861 2531 Fragments arranged by size: 2531 1861 1390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 4423 4455 4497 Size: 32 135 32 962 32 1857 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1857 962 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 3064 3064 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 2846 3740 4099 4258 518 Size: 648 1578 102 894 359 159 2042 Fragments arranged by size: 2042 1578 894 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2422 2932 3198 3875 5023 5268 1606 Size: 816 510 266 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 510 266 245 NarI GG'CG_CC Cuts at: 3065 3065 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3048 3140 4685 1132 Size: 97 1819 92 1545 2229 Fragments arranged by size: 2229 1819 1545 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PfoI T'CCnGG_A Cuts at: 2907 2907 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2916 1613 Size: 1303 4479 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2329 2746 3134 830 Size: 1499 417 388 3478 Fragments arranged by size: 3478 1499 417 388 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2422 2932 2422 Size: 510 5272 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2325 2533 2742 2835 2866 3130 4265 Size: 219 1280 208 209 93 31 264 1135 Cuts at: 4265 4946 5137 826 Size: 681 191 1471 Fragments arranged by size: 1471 1280 1135 681 264 219 209 208 191 93 31 SfoI GGC'GCC Cuts at: 3066 3066 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3048 3140 1132 Size: 1916 92 3774 Fragments arranged by size: 3774 1916 92 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2714 1517 Size: 1197 4585 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2047 3031 3221 513 Size: 552 939 6 37 984 190 3074 Fragments arranged by size: 3074 984 939 552 190 37 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3809 3826 3979 4164 4609 325 Size: 1255 2229 17 153 185 445 1498 Fragments arranged by size: 2229 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI Bsu36I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI