Restriction map for
pFN21AE3822
(Linear) (Six-base) MAPSORT of: KIEE3822.seq Check: 6022 from: 1 to: 7581
KIEE3822.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 225 7581
Size: 225 7356
AccI GT'mk_AC
Cuts at: 0 7076 7326 7581
Size: 7076 250 255
Fragments arranged by size:
7076 255 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 416 427 1388 2615 2846 2857 3121
Size: 67 349 11 961 1227 231 11 264
Cuts at: 3121 3403 3412 4298 5252 5276 5860 6143 6578
Size: 282 9 886 954 24 584 283 435
Cuts at: 6578 7240 7460 7581
Size: 662 220 121
Fragments arranged by size:
1227 961 954 886 662 584 435 349
283 282 264 231 220 121 67 24
11 11 9
AfeI AGC'GCT
Cuts at: 0 935 7581
Size: 935 6646
AflII C'TTAA_G
Cuts at: 0 2370 3663 7581
Size: 2370 1293 3918
Fragments arranged by size:
3918 2370 1293
AflIII A'CryG_T
Cuts at: 0 552 4934 7335 7581
Size: 552 4382 2401 246
Fragments arranged by size:
4382 2401 552 246
AleI CACnn'nnGTG
Cuts at: 0 252 3310 7581
Size: 252 3058 4271
Fragments arranged by size:
4271 3058 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5936 7581
Size: 5936 1645
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3538 3570 7581
Size: 3538 32 4011
Fragments arranged by size:
4011 3538 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1812 7581
Size: 1812 5769
ApaI G_GGCC'C
Cuts at: 0 2813 7581
Size: 2813 4768
ApaLI G'TGCA_C
Cuts at: 0 6116 7168 7581
Size: 6116 1052 413
Fragments arranged by size:
6116 1052 413
ApoI r'AATT_y
Cuts at: 0 592 639 794 1573 1802 1886 2586 2611
Size: 592 47 155 779 229 84 700 25
Cuts at: 2611 2743 2781 3136 3486 3550 4198 4630 4897
Size: 132 38 355 350 64 648 432 267
Cuts at: 4897 4918 4963 5626 6396 6780 6921 7515 7581
Size: 21 45 663 770 384 141 594 66
Fragments arranged by size:
779 770 700 663 648 594 592 432
384 355 350 267 229 155 141 132
84 66 64 47 45 38 25 21
AseI AT'TA_AT
Cuts at: 0 262 4060 5122 6328 7581
Size: 262 3798 1062 1206 1253
Fragments arranged by size:
3798 1253 1206 1062 262
AvrII C'CTAG_G
Cuts at: 0 5652 7581
Size: 5652 1929
BanII G_rGCy'C
Cuts at: 0 2813 7581
Size: 2813 4768
BbsI GAAGACnn'nnnn_
Cuts at: 0 415 2030 7581
Size: 415 1615 5551
Fragments arranged by size:
5551 1615 415
BbvCI CC'TCA_GC
Cuts at: 0 4261 7581
Size: 4261 3320
BciVI GTATCCnnnnn_n'
Cuts at: 0 1560 1796 1825 6026 6556 7581
Size: 1560 236 29 4201 530 1025
Fragments arranged by size:
4201 1560 1025 530 236 29
BclI T'GATC_A
Cuts at: 0 2151 2528 2820 7581
Size: 2151 377 292 4761
Fragments arranged by size:
4761 2151 377 292
BfrBI ATG'CAT
Cuts at: 0 1221 1261 1726 6461 7581
Size: 1221 40 465 4735 1120
Fragments arranged by size:
4735 1221 1120 465 40
BglII A'GATC_T
Cuts at: 0 417 926 4080 4158 5511 7581
Size: 417 509 3154 78 1353 2070
Fragments arranged by size:
3154 2070 1353 509 417 78
BlpI GC'TnA_GC
Cuts at: 0 937 6603 7097 7581
Size: 937 5666 494 484
Fragments arranged by size:
5666 937 494 484
Bme1580I G_kGCm'C
Cuts at: 0 1119 2813 6103 6120 6306 6518 7172 7581
Size: 1119 1694 3290 17 186 212 654 409
Fragments arranged by size:
3290 1694 1119 654 409 212 186 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 4037 5783 7581
Size: 4037 1746 1798
Fragments arranged by size:
4037 1798 1746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 588 5865 6550 7581
Size: 168 420 5277 685 1031
Fragments arranged by size:
5277 1031 685 420 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1525 3067 3346 5407 5624 6227 7307
Size: 136 1389 1542 279 2061 217 603 1080
Cuts at: 7307 7581
Size: 274
Fragments arranged by size:
2061 1542 1389 1080 603 279 274 217
136
Bpu10I CC'TnA_GC
Cuts at: 0 2998 4261 7581
Size: 2998 1263 3320
Fragments arranged by size:
3320 2998 1263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3396 3466 3729 5158 6341 7581
Size: 3396 70 263 1429 1183 1240
Fragments arranged by size:
3396 1429 1240 1183 263 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 5231 5972 7581
Size: 5231 741 1609
Fragments arranged by size:
5231 1609 741
BsaAI yAC'GTr
Cuts at: 0 137 1095 5931 7336 7581
Size: 137 958 4836 1405 245
Fragments arranged by size:
4836 1405 958 245 137
BsaBI GATnn'nnATC
Cuts at: 0 2204 3697 7581
Size: 2204 1493 3884
Fragments arranged by size:
3884 2204 1493
BsaWI w'CCGG_w
Cuts at: 0 4646 7427 7581
Size: 4646 2781 154
Fragments arranged by size:
4646 2781 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5690 5720 7581
Size: 5690 30 1861
Fragments arranged by size:
5690 1861 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 592 943 7304 7581
Size: 412 180 351 6361 277
Fragments arranged by size:
6361 412 351 277 180
BseYI C'CCAG_C
Cuts at: 0 1112 1185 4761 5812 6504 7581
Size: 1112 73 3576 1051 692 1077
Fragments arranged by size:
3576 1112 1077 1051 692 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 916 1117 1519 7581
Size: 250 666 201 402 6062
Fragments arranged by size:
6062 666 402 250 201
BsiHKAI G_wGCw'C
Cuts at: 0 149 5178 6120 7172 7581
Size: 149 5029 942 1052 409
Fragments arranged by size:
5029 1052 942 409 149
BsmI GAATG_Cn'
Cuts at: 0 775 918 1221 1263 2182 2907 3066 4094
Size: 775 143 303 42 919 725 159 1028
Cuts at: 4094 4470 6463 6495 7581
Size: 376 1993 32 1086
Fragments arranged by size:
1993 1086 1028 919 775 725 376 303
159 143 42 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 6417 7557 7581
Size: 6417 1140 24
Fragments arranged by size:
6417 1140 24
Bsp1286I G_dGCh'C
Cuts at: 0 149 1119 2813 5178 6103 6120 6306 6518
Size: 149 970 1694 2365 925 17 186 212
Cuts at: 6518 7172 7581
Size: 654 409
Fragments arranged by size:
2365 1694 970 925 654 409 212 186
149 17
BspHI T'CATG_A
Cuts at: 0 725 1479 2823 7370 7581
Size: 725 754 1344 4547 211
Fragments arranged by size:
4547 1344 754 725 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 225 4177 5597 7581
Size: 225 3952 1420 1984
Fragments arranged by size:
3952 1984 1420 225
BsrDI GCAATG_nn'
Cuts at: 0 741 919 3621 6032 6056 7581
Size: 741 178 2702 2411 24 1525
Fragments arranged by size:
2702 2411 1525 741 178 24
BsrGI T'GTAC_A
Cuts at: 0 1710 7581
Size: 1710 5871
BssSI C'ACGA_G
Cuts at: 0 2297 3069 7581
Size: 2297 772 4512
Fragments arranged by size:
4512 2297 772
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4391 6043 7581
Size: 4391 1652 1538
Fragments arranged by size:
4391 1652 1538
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 535 2049 2271 2805 3861 7581
Size: 313 222 1514 222 534 1056 3720
Fragments arranged by size:
3720 1514 1056 534 313 222 222
BstYI r'GATC_y
Cuts at: 0 120 417 926 2221 2293 3228 3844 3885
Size: 120 297 509 1295 72 935 616 41
Cuts at: 3885 4080 4158 4535 5251 5511 7311 7581
Size: 195 78 377 716 260 1800 270
Fragments arranged by size:
1800 1295 935 716 616 509 377 297
270 260 195 120 78 72 41
Bsu36I CC'TnA_GG
Cuts at: 0 2571 4865 7581
Size: 2571 2294 2716
Fragments arranged by size:
2716 2571 2294
BtgI C'CryG_G
Cuts at: 0 6307 7581
Size: 6307 1274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2594 7581
Size: 2594 4987
BtsI GCAGTG_nn'
Cuts at: 0 1451 1774 4765 7079 7265 7581
Size: 1451 323 2991 2314 186 316
Fragments arranged by size:
2991 2314 1451 323 316 186
ClaI AT'CG_AT
Cuts at: 0 5130 7581
Size: 5130 2451
DraI TTT'AAA
Cuts at: 0 645 3401 3578 3924 7581
Size: 645 2756 177 346 3657
Fragments arranged by size:
3657 2756 645 346 177
DraIII CAC_nnn'GTG
Cuts at: 0 4461 5997 7581
Size: 4461 1536 1584
Fragments arranged by size:
4461 1584 1536
EaeI y'GGCC_r
Cuts at: 0 1176 2393 7581
Size: 1176 1217 5188
Fragments arranged by size:
5188 1217 1176
EarI CTCTTCn'nnn_
Cuts at: 0 23 647 2646 3097 4309 7581
Size: 23 624 1999 451 1212 3272
Fragments arranged by size:
3272 1999 1212 624 451 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 136 416 427 1388 1525 2615 2846
Size: 67 69 280 11 961 137 1090 231
Cuts at: 2846 2857 3067 3121 3346 3403 3412 4298 5252
Size: 11 210 54 225 57 9 886 954
Cuts at: 5252 5276 5407 5624 5860 6143 6227 6578 7240
Size: 24 131 217 236 283 84 351 662
Cuts at: 7240 7307 7460 7581
Size: 67 153 121
Fragments arranged by size:
1090 961 954 886 662 351 283 280
236 231 225 217 210 153 137 131
121 84 69 67 67 57 54 24
11 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4264 7581
Size: 4264 3317
EcoO109I rG'GnC_Cy
Cuts at: 0 301 2809 3637 7581
Size: 301 2508 828 3944
Fragments arranged by size:
3944 2508 828 301
EcoRI G'AATT_C
Cuts at: 0 794 4198 6396 7581
Size: 794 3404 2198 1185
Fragments arranged by size:
3404 2198 1185 794
EcoRV GAT'ATC
Cuts at: 0 2536 5798 7581
Size: 2536 3262 1783
Fragments arranged by size:
3262 2536 1783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1439 5877 6386 7581
Size: 1439 4438 509 1195
Fragments arranged by size:
4438 1439 1195 509
HaeII r_GCGC'y
Cuts at: 0 937 1513 7581
Size: 937 576 6068
Fragments arranged by size:
6068 937 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 1723 1755 2520 2552 2590 2622
Size: 402 32 1289 32 765 32 38 32
Cuts at: 2622 3780 3812 4148 4180 4527 4559 4662 4694
Size: 1158 32 336 32 347 32 103 32
Cuts at: 4694 5243 5275 6125 6157 7453 7485 7581
Size: 549 32 850 32 1296 32 96
Fragments arranged by size:
1296 1289 1158 850 765 549 402 347
336 103 96 38 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 635 1268 2324 7327 7581
Size: 635 633 1056 5003 254
Fragments arranged by size:
5003 1056 635 633 254
HindIII A'AGCT_T
Cuts at: 0 291 545 5234 6841 7581
Size: 291 254 4689 1607 740
Fragments arranged by size:
4689 1607 740 291 254
HpaI GTT'AAC
Cuts at: 0 635 7581
Size: 635 6946
MfeI C'AATT_G
Cuts at: 0 346 4625 7581
Size: 346 4279 2956
Fragments arranged by size:
4279 2956 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 3271 4536 5100 5603 6774 7079 7581
Size: 294 2977 1265 564 503 1171 305 502
Fragments arranged by size:
2977 1265 1171 564 503 502 305 294
MscI TGG'CCA
Cuts at: 0 1178 2395 7581
Size: 1178 1217 5186
Fragments arranged by size:
5186 1217 1178
MslI CAynn'nnrTG
Cuts at: 0 252 1071 1216 1328 3059 3310 3726 4114
Size: 252 819 145 112 1731 251 416 388
Cuts at: 4114 4361 6900 7066 7534 7581
Size: 247 2539 166 468 47
Fragments arranged by size:
2539 1731 819 468 416 388 252 251
247 166 145 112 47
MspA1I CmG'CkG
Cuts at: 0 14 941 1503 3282 4563 4683 5812 6403
Size: 14 927 562 1779 1281 120 1129 591
Cuts at: 6403 6802 7581
Size: 399 779
Fragments arranged by size:
1779 1281 1129 927 779 591 562 399
120 14
NcoI C'CATG_G
Cuts at: 0 6307 7581
Size: 6307 1274
NdeI CA'TA_TG
Cuts at: 0 2900 7581
Size: 2900 4681
NruI TCG'CGA
Cuts at: 0 223 7581
Size: 223 7358
NsiI A_TGCA'T
Cuts at: 0 1223 1263 1728 6463 7581
Size: 1223 40 465 4735 1118
Fragments arranged by size:
4735 1223 1118 465 40
NspI r_CATG'y
Cuts at: 0 556 1112 1548 4938 5157 7581
Size: 556 556 436 3390 219 2424
Fragments arranged by size:
3390 2424 556 556 436 219
PacI TTA_AT'TAA
Cuts at: 0 6332 7581
Size: 6332 1249
PciI A'CATG_T
Cuts at: 0 552 4934 7581
Size: 552 4382 2647
Fragments arranged by size:
4382 2647 552
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 5340 7581
Size: 114 5226 2241
Fragments arranged by size:
5226 2241 114
PfoI T'CCnGG_A
Cuts at: 0 77 113 7307 7581
Size: 77 36 7194 274
Fragments arranged by size:
7194 274 77 36
PmlI CAC'GTG
Cuts at: 0 1095 7581
Size: 1095 6486
PpuMI rG'GwC_Cy
Cuts at: 0 301 3637 7581
Size: 301 3336 3944
Fragments arranged by size:
3944 3336 301
PsiI TTA'TAA
Cuts at: 0 749 3995 6149 7581
Size: 749 3246 2154 1432
Fragments arranged by size:
3246 2154 1432 749
PspOMI G'GGCC_C
Cuts at: 0 2809 7581
Size: 2809 4772
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2294 2326 7581
Size: 2294 32 5255
Fragments arranged by size:
5255 2294 32
PstI C_TGCA'G
Cuts at: 0 1781 4688 4772 7581
Size: 1781 2907 84 2809
Fragments arranged by size:
2907 2809 1781 84
PvuII CAG'CTG
Cuts at: 0 14 941 1503 3282 4563 4683 5812 6403
Size: 14 927 562 1779 1281 120 1129 591
Cuts at: 6403 7581
Size: 1178
Fragments arranged by size:
1779 1281 1178 1129 927 591 562 120
14
SalI G'TCGA_C
Cuts at: 0 7325 7581
Size: 7325 256
SanDI GG'GwC_CC
Cuts at: 0 301 7581
Size: 301 7280
SapI GCTCTTCn'nnn_
Cuts at: 0 4309 7581
Size: 4309 3272
ScaI AGT'ACT
Cuts at: 0 4209 6067 6891 7581
Size: 4209 1858 824 690
Fragments arranged by size:
4209 1858 824 690
SexAI A'CCwGG_T
Cuts at: 0 3633 7581
Size: 3633 3948
SfcI C'TryA_G
Cuts at: 0 1777 3233 4203 4684 4768 5908 6072 7581
Size: 1777 1456 970 481 84 1140 164 1509
Fragments arranged by size:
1777 1509 1456 1140 970 481 164 84
SmlI C'TyrA_G
Cuts at: 0 2370 3375 3445 3663 3708 5137 6356 7581
Size: 2370 1005 70 218 45 1429 1219 1225
Fragments arranged by size:
2370 1429 1225 1219 1005 218 70 45
SpeI A'CTAG_T
Cuts at: 0 4665 7581
Size: 4665 2916
SphI G_CATG'C
Cuts at: 0 1112 1548 7581
Size: 1112 436 6033
Fragments arranged by size:
6033 1112 436
SspI AAT'ATT
Cuts at: 0 753 2024 2855 4996 5601 6596 7581
Size: 753 1271 831 2141 605 995 985
Fragments arranged by size:
2141 1271 995 985 831 753 605
StuI AGG'CCT
Cuts at: 0 5031 6366 7581
Size: 5031 1335 1215
Fragments arranged by size:
5031 1335 1215
StyI C'CwwG_G
Cuts at: 0 333 2412 2804 4297 4409 5536 5652 5852
Size: 333 2079 392 1493 112 1127 116 200
Cuts at: 5852 5861 6307 6608 7581
Size: 9 446 301 973
Fragments arranged by size:
2079 1493 1127 973 446 392 333 301
200 116 112 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7350 7581
Size: 7350 231
TatI w'GTAC_w
Cuts at: 0 1710 4207 4784 6065 6889 7581
Size: 1710 2497 577 1281 824 692
Fragments arranged by size:
2497 1710 1281 824 692 577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3060 3092 3203 3235 3409 3441 4786 4818
Size: 3060 32 111 32 174 32 1345 32
Cuts at: 4818 5690 5722 6000 6032 7581
Size: 872 32 278 32 1549
Fragments arranged by size:
3060 1549 1345 872 278 174 111 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 6657 7581
Size: 6657 924
XbaI T'CTAG_A
Cuts at: 0 3733 7581
Size: 3733 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5108 5859 5935 7581
Size: 5108 751 76 1646
Fragments arranged by size:
5108 1646 751 76
XmnI GAAnn'nnTTC
Cuts at: 0 775 794 1654 1855 2589 4875 4898 5372
Size: 775 19 860 201 734 2286 23 474
Cuts at: 5372 7581
Size: 2209
Fragments arranged by size:
2286 2209 860 775 734 474 201 23
19
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvrII BanII
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I NcoI NdeI NruI NsiI NspI PacI
PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI
PstI PvuII SalI SanDI SapI ScaI SexAI SfcI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AscI AsiSI AvaI
BaeI BamHI BanI BbeI BcgI BglI BmgBI BmtI
BsaHI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI
BstEII BstZ17I CspCI DrdI EagI EciI EcoICRI FseI
FspI FspAI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI PasI PmeI PpiI PshAI PspXI PvuI
RsrII SacI SacII SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI SwaI XhoI XmaI ZraI