Restriction map for
pFN21AE1100
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 768 AAATTC [GA]^AATT[CT]
Alw44I 678 GTGCAC G^TGCAC
AlwNI 143 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 678 GTGCAC G^TGCAC
ApoI 768 AAATTC [GA]^AATT[CT]
AspHI 678 GTGCAC G[AT]GC[AT]^C
AspI 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 270 GGTCC G^G[AT]CC
BamHI 9 GGATCC G^GATCC
BcnI 273 CCCGG CC^[GC]GG
BfaI 842 CTAG C^TAG
BmyI 678 GTGCAC G[AGT]GC[ACT]^C
BseRI 155 CTCCTC GAGGAG(10/8)
BsiHKAI 678 GTGCAC G[AT]GC[AT]^C
BsiYI 683 CCCAATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 683 CCCAATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 678 GTGCAC G[AGT]GC[ACT]^C
BsrDI 485 GCAATG GCAATG(2/0)
BstXI 779 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 269 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 472 TGGCCG [CT]^GGCC[GA]
EcoO109I 269 AGGTCCC [GA]G^G[ACGT]CC[CT]
HapII 274 CCGG C^CGG
HgaI 495 GACGC GACGC(5/10)
HindIII 27 AAGCTT A^AGCTT
HpaII 274 CCGG C^CGG
MaeI 842 CTAG C^TAG
MaeII 911 ACGT A^CGT
MseI 821 TTAA T^TAA
MslI 891 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 316 CAGCTG C[AC]G^C[GT]G
MspI 274 CCGG C^CGG
NciI 273 CCCGG CC^[GC]GG
PflFI 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 269 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 316 CAGCTG CAG^CTG
ScaI 853 AGTACT AGT^ACT
SphI 752 GCATGC GCATG^C
Tru9I 821 TTAA T^TAA
TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 265 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 325 GTAC GT^AC
854 GTAC
BbvI 793 GCTGC GCAGC(8/12)
805 GCTGC
BstYI 9 GGATCC [GA]^GATC[CT]
174 GGATCT
Cac8I 29 GCTTGC GC[ACGT]^[ACGT]GC
752 GCATGC
Cfr13I 88 GGGCC G^G[ACGT]CC
270 GGTCC
DpnII 10 GATC ^GATC
175 GATC
DsaI 170 CCATGG C^C[GA][CT]GG
866 CCATGG
Fnu4HI 793 GCTGC GC^[ACGT]GC
805 GCTGC
ItaI 793 GCTGC GC^[ACGT]GC
805 GCTGC
MaeIII 493 GTGAC ^GT[ACGT]AC
782 GTGAC
MboI 10 GATC ^GATC
175 GATC
MflI 9 GGATCC [GA]^GATC[CT]
174 GGATCT
NcoI 170 CCATGG C^CATGG
866 CCATGG
NdeII 10 GATC ^GATC
175 GATC
NspI 752 GCATGC [GA]CATG^[CT]
896 ACATGC
PstI 236 CTGCAG CTGCA^G
742 CTGCAG
RsaI 325 GTAC GT^AC
854 GTAC
Sau3AI 10 GATC ^GATC
175 GATC
Sau96I 88 GGGCC G^G[ACGT]CC
270 GGTCC
SexAI 421 ACCTGGT A^CC[AT]GGT
673 ACCTGGT
SfcI 236 CTGCAG C^T[GA][CT]AG
742 CTGCAG
TseI 793 GCTGC G^C[AT]GC
805 GCTGC
Tsp45I 493 GTGAC ^GT[GC]AC
782 GTGAC
Tsp509I 607 AATT ^AATT
769 AATT
XhoII 9 GGATCC [GA]^GATC[CT]
174 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AlwI BbsI BbvCI BpmI
BpuAI BsaJI BsmAI BsmFI BsrI
BssKI BstF5I BstNI DdeI EarI
EcoRII EcoT14I FokI HaeIII HinfI
HphI Ksp632I MboII MnlI MvaI
MwoI NlaIII NlaIV ScrFI SfaNI
StuI StyI TfiI