(Linear) (Six-base) MAPSORT of: KIEE2053.seq Check: 7175 from: 1 to: 2064 KIEE2053.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 251 2064 Size: 155 96 1813 Fragments arranged by size: 1813 155 96 AfeI AGC'GCT Cuts at: 0 1388 2064 Size: 1388 676 AflIII A'CryG_T Cuts at: 0 990 2064 Size: 990 1074 AhdI GACnn_n'nnGTC Cuts at: 0 1630 2064 Size: 1630 434 AleI CACnn'nnGTG Cuts at: 0 1471 2064 Size: 1471 593 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 616 648 2064 Size: 616 32 1416 Fragments arranged by size: 1416 616 32 ApaI G_GGCC'C Cuts at: 0 1246 1970 2064 Size: 1246 724 94 Fragments arranged by size: 1246 724 94 ApoI r'AATT_y Cuts at: 0 1936 2064 Size: 1936 128 AseI AT'TA_AT Cuts at: 0 1394 2064 Size: 1394 670 BamHI G'GATC_C Cuts at: 0 1321 1917 2064 Size: 1321 596 147 Fragments arranged by size: 1321 596 147 BanI G'GyrC_C Cuts at: 0 1654 2064 Size: 1654 410 BanII G_rGCy'C Cuts at: 0 1246 1970 1991 2064 Size: 1246 724 21 73 Fragments arranged by size: 1246 724 73 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 896 1118 1350 2035 2064 Size: 896 222 232 685 29 Fragments arranged by size: 896 685 232 222 29 BglI GCCn_nnn'nGGC Cuts at: 0 1652 2064 Size: 1652 412 BlpI GC'TnA_GC Cuts at: 0 44 2064 Size: 44 2020 Bme1580I G_kGCm'C Cuts at: 0 1246 1970 2064 Size: 1246 724 94 Fragments arranged by size: 1246 724 94 BmgBI CAC'GTC Cuts at: 0 991 2064 Size: 991 1073 BmrI ACTGGGnnnn_n' Cuts at: 0 92 1413 1606 1965 2064 Size: 92 1321 193 359 99 Fragments arranged by size: 1321 359 193 99 92 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 815 2064 Size: 815 1249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 1877 2064 Size: 301 1576 187 Fragments arranged by size: 1576 301 187 Bpu10I CC'TnA_GC Cuts at: 0 1514 2064 Size: 1514 550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 1729 2064 Size: 582 1147 335 Fragments arranged by size: 1147 582 335 BsaWI w'CCGG_w Cuts at: 0 617 2064 Size: 617 1447 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 55 85 231 261 451 481 616 646 Size: 55 30 146 30 190 30 135 30 Cuts at: 646 1620 1650 2064 Size: 974 30 414 Fragments arranged by size: 974 414 190 146 135 55 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 270 1174 1202 1874 2064 Size: 270 904 28 672 190 Fragments arranged by size: 904 672 270 190 28 BseYI C'CCAG_C Cuts at: 0 1223 1239 1706 2064 Size: 1223 16 467 358 Fragments arranged by size: 1223 467 358 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 527 970 1495 2064 Size: 229 298 443 525 569 Fragments arranged by size: 569 525 443 298 229 BsiHKAI G_wGCw'C Cuts at: 0 1126 2064 Size: 1126 938 BsmI GAATG_Cn' Cuts at: 0 406 1079 2064 Size: 406 673 985 Fragments arranged by size: 985 673 406 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1638 2064 Size: 1638 426 Bsp1286I G_dGCh'C Cuts at: 0 1126 1246 1970 1991 2064 Size: 1126 120 724 21 73 Fragments arranged by size: 1126 724 120 73 21 BsrDI GCAATG_nn' Cuts at: 0 1035 1586 2064 Size: 1035 551 478 Fragments arranged by size: 1035 551 478 BsrGI T'GTAC_A Cuts at: 0 1911 2064 Size: 1911 153 BstEII G'GTnAC_C Cuts at: 0 645 1757 2064 Size: 645 1112 307 Fragments arranged by size: 1112 645 307 BstYI r'GATC_y Cuts at: 0 65 614 867 1321 1917 2064 Size: 65 549 253 454 596 147 Fragments arranged by size: 596 549 454 253 147 65 Bsu36I CC'TnA_GG Cuts at: 0 86 884 2064 Size: 86 798 1180 Fragments arranged by size: 1180 798 86 BtgI C'CryG_G Cuts at: 0 1372 2064 Size: 1372 692 BtsI GCAGTG_nn' Cuts at: 0 531 539 2064 Size: 531 8 1525 Fragments arranged by size: 1525 531 8 EarI CTCTTCn'nnn_ Cuts at: 0 284 344 1665 1717 2064 Size: 284 60 1321 52 347 Fragments arranged by size: 1321 347 284 60 52 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 251 301 1877 2064 Size: 155 96 50 1576 187 Fragments arranged by size: 1576 187 155 96 50 EcoO109I rG'GnC_Cy Cuts at: 0 1624 1686 1901 1966 1967 1979 2064 Size: 1624 62 215 65 1 12 85 Fragments arranged by size: 1624 215 85 65 62 12 1 EcoRV GAT'ATC Cuts at: 0 807 2064 Size: 807 1257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 354 2064 Size: 354 1710 HaeII r_GCGC'y Cuts at: 0 1390 2064 Size: 1390 674 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 549 581 945 977 1404 1436 Size: 55 32 462 32 364 32 427 32 Cuts at: 1436 1494 1526 1616 1648 2064 Size: 58 32 90 32 416 Fragments arranged by size: 462 427 416 364 90 58 55 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1063 2016 2064 Size: 1063 953 48 Fragments arranged by size: 1063 953 48 MfeI C'AATT_G Cuts at: 0 281 2064 Size: 281 1783 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 602 1688 2064 Size: 602 1086 376 Fragments arranged by size: 1086 602 376 MslI CAynn'nnrTG Cuts at: 0 1471 2064 Size: 1471 593 MspA1I CmG'CkG Cuts at: 0 682 691 1948 2064 Size: 682 9 1257 116 Fragments arranged by size: 1257 682 116 9 NcoI C'CATG_G Cuts at: 0 1372 2064 Size: 1372 692 NspI r_CATG'y Cuts at: 0 1522 2064 Size: 1522 542 PasI CC'CwG_GG Cuts at: 0 766 2064 Size: 766 1298 PflMI CCAn_nnn'nTGG Cuts at: 0 642 1273 1378 2064 Size: 642 631 105 686 Fragments arranged by size: 686 642 631 105 PfoI T'CCnGG_A Cuts at: 0 1533 2064 Size: 1533 531 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 616 648 2064 Size: 616 32 1416 Fragments arranged by size: 1416 616 32 PpuMI rG'GwC_Cy Cuts at: 0 1624 1686 1979 2064 Size: 1624 62 293 85 Fragments arranged by size: 1624 293 85 62 PspOMI G'GGCC_C Cuts at: 0 1242 1966 2064 Size: 1242 724 98 Fragments arranged by size: 1242 724 98 PvuII CAG'CTG Cuts at: 0 682 1948 2064 Size: 682 1266 116 Fragments arranged by size: 1266 682 116 SanDI GG'GwC_CC Cuts at: 0 1624 2064 Size: 1624 440 SapI GCTCTTCn'nnn_ Cuts at: 0 344 2064 Size: 344 1720 ScaI AGT'ACT Cuts at: 0 1402 2064 Size: 1402 662 SfcI C'TryA_G Cuts at: 0 1763 2064 Size: 1763 301 SmlI C'TyrA_G Cuts at: 0 597 1744 2064 Size: 597 1147 320 Fragments arranged by size: 1147 597 320 SspI AAT'ATT Cuts at: 0 484 780 2064 Size: 484 296 1284 Fragments arranged by size: 1284 484 296 StuI AGG'CCT Cuts at: 0 1825 2064 Size: 1825 239 StyI C'CwwG_G Cuts at: 0 1372 2064 Size: 1372 692 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 178 1215 1395 2064 Size: 178 1037 180 669 Fragments arranged by size: 1037 669 180 178 TatI w'GTAC_w Cuts at: 0 1400 1911 2064 Size: 1400 511 153 Fragments arranged by size: 1400 511 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 231 263 1620 1652 2064 Size: 231 32 1357 32 412 Fragments arranged by size: 1357 412 231 32 32 XmnI GAAnn'nnTTC Cuts at: 0 631 2064 Size: 631 1433 Enzymes that do cut: AcuI AfeI AflIII AhdI AleI AloI ApaI ApoI AseI BamHI BanI BanII BbsI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstEII BstYI Bsu36I BtgI BtsI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII MfeI MmeI MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PvuII SanDI SapI ScaI SfcI SmlI SspI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AlfI AlwNI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI