################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau96I ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 493 GTCTAC GT^[AC][GT]AC AciI 414 CCGC CCGC(-3/-1) AlwNI 762 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 921 CCTGAGG CC^T[ACGT]AGG AspHI 559 GAGCAC G[AT]GC[AT]^C AvaI 145 CTCGGG C^[CT]CG[GA]G BbsI 643 GTCTTC GAAGAC(2/6) BbvCI 960 GCTGAGG CCTCAGC(-5/-2) BclI 185 TGATCA T^GATCA BfaI 617 CTAG C^TAG BmyI 559 GAGCAC G[AGT]GC[ACT]^C BpuAI 643 GTCTTC GAAGAC(2/6) BsiEI 411 CGACCG CG[GA][CT]^CG BsiHKAI 559 GAGCAC G[AT]GC[AT]^C BsmI 814 GCATTC GAATGC(1/-1) BsoBI 145 CTCGGG C^[CT]CG[GA]G Bsp1286I 559 GAGCAC G[AGT]GC[ACT]^C BspHI 599 TCATGA T^CATGA BsrDI 375 CATTGC GCAATG(2/0) BsrI 529 ACTGG ACTGG(1/-1) BssSI 675 CACGAG CACGAG(-5/-1) BstEII 159 GGTCACC G^GT[ACGT]ACC BstPI 159 GGTCACC G^GT[ACGT]ACC Bsu36I 921 CCTGAGG CC^T[ACGT]AGG Eco81I 921 CCTGAGG CC^T[ACGT]AGG EcoO65I 159 GGTCACC G^GT[ACGT]ACC EcoT22I 19 ATGCAT ATGCA^T FbaI 185 TGATCA T^GATCA MaeI 617 CTAG C^TAG MspA1I 211 CAGCTG C[AC]G^C[GT]G NsiI 19 ATGCAT ATGCA^T PleI 506 GACTC GAGTC(4/5) PvuII 211 CAGCTG CAG^CTG RcaI 599 TCATGA T^CATGA SbfI 26 CCTGCAGG CCTGCA^GG SmlI 91 CTCAAG C^T[CT][GA]AG Sse8387I 26 CCTGCAGG CCTGCA^GG StuI 31 AGGCCT AGG^CCT Tsp509I 195 AATT ^AATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 228 GTAC GT^AC 822 GTAC BpmI 118 CTCCAG CTGGAG(16/14) 714 CTCCAG BsgI 214 CTGCAC GTGCAG(16/14) 949 GTGCAG BsmAI 12 GTCTC GTCTC(1/5) 459 GTCTC BspMI 469 ACCTGC ACCTGC(4/8) 766 ACCTGC BssKI 177 CCTGG ^CC[ACGT]GG 855 CCAGG BstNI 177 CCTGG CC^[AT]GG 855 CCAGG Cac8I 29 GCAGGC GC[ACGT]^[ACGT]GC 473 GCCTGC DsaI 788 CCGTGG C^C[GA][CT]GG 884 CCACGG EarI 139 CTCTTC CTCTTC(1/4) 105 GAAGAG EcoRII 177 CCTGG ^CC[AT]GG 855 CCAGG HphI 297 GGTGA GGTGA(8/7) 161 TCACC Ksp632I 139 CTCTTC CTCTTC(1/4) 105 GAAGAG MvaI 177 CCTGG CC^[AT]GG 855 CCAGG NlaIV 173 GGAACC GG[ACGT]^[ACGT]CC 514 GGCTCC NspI 341 GCATGT [GA]CATG^[CT] 389 ACATGC RsaI 228 GTAC GT^AC 822 GTAC ScrFI 177 CCTGG CC^[ACGT]GG 855 CCAGG TfiI 659 GATTC G^A[AT]TC 903 GAATC TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^ 398 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI AlwI BbvI BsaJI BseRI BsiYI BslI BstF5I BstYI DdeI DpnII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII PstI Sau3AI SfaNI SfcI Tru9I TseI Tsp45I XhoII