(Circular) (Six-base) MAPSORT of: pFN21AE6094.seq Check: 9905 from: 1 to: 5683 pFN21AE6094 5683 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2780 2483 Size: 297 5386 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 3026 1937 Size: 562 527 4594 Fragments arranged by size: 4594 562 527 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2749 3746 5130 5398 1632 Size: 1117 997 1384 268 1917 Fragments arranged by size: 1917 1384 1117 997 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 2171 1166 Size: 1005 4678 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2772 3426 4998 2772 Size: 654 1572 3457 Fragments arranged by size: 3457 1572 654 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2150 3008 1870 Size: 73 207 858 4545 Fragments arranged by size: 4545 858 207 73 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 4459 618 Size: 325 217 627 1217 1455 1842 Fragments arranged by size: 1842 1455 1217 627 325 217 BanII G_rGCy'C Cuts at: 1391 3002 1391 Size: 1611 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2641 928 Size: 971 742 3970 Fragments arranged by size: 3970 971 742 BbvCI CC'TCA_GC Cuts at: 2966 2966 Size: 5683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 5041 BfrBI ATG'CAT Cuts at: 2026 2026 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 3744 4900 1790 Size: 1954 1156 2573 Fragments arranged by size: 2573 1954 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2138 2138 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2704 4343 1239 Size: 869 596 1639 2579 Fragments arranged by size: 2579 1639 869 596 Bpu10I CC'TnA_GC Cuts at: 2966 2966 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 3808 4673 4971 2081 Size: 1727 865 298 2793 Fragments arranged by size: 2793 1727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4516 Fragments arranged by size: 4516 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2065 2132 2266 1186 Size: 879 67 134 4603 Fragments arranged by size: 4603 879 134 67 BseYI C'CCAG_C Cuts at: 4886 5382 4886 Size: 496 5187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2975 1163 Size: 573 468 771 3871 Fragments arranged by size: 3871 771 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3133 3894 4922 1540 Size: 275 186 418 714 761 1028 2301 Fragments arranged by size: 2301 1028 761 714 418 275 186 BsiHKAI G_wGCw'C Cuts at: 2568 3744 3829 4900 2568 Size: 1176 85 1071 3351 Fragments arranged by size: 3351 1176 1071 85 BsmI GAATG_Cn' Cuts at: 2819 3194 3287 2819 Size: 375 93 5215 Fragments arranged by size: 5215 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2568 3002 3744 3829 4900 1391 Size: 399 778 434 742 85 1071 2174 Fragments arranged by size: 2174 1071 778 742 434 399 85 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 2604 3572 5302 2604 Size: 968 1730 2985 Fragments arranged by size: 2985 1730 968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 2780 3038 844 Size: 1639 297 258 3489 Fragments arranged by size: 3489 1639 297 258 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2379 4178 4352 65 Size: 1183 1131 1799 174 1396 Fragments arranged by size: 1799 1396 1183 1131 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 2680 3743 4755 2680 Size: 1063 1012 3608 Fragments arranged by size: 3608 1063 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2164 1184 Size: 980 4703 BstYI r'GATC_y Cuts at: 1972 2154 2263 2753 3013 3380 3765 3782 5234 Size: 182 109 490 260 367 385 17 1452 Cuts at: 5234 5320 5332 5678 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 490 385 367 346 260 182 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2927 2927 Size: 5683 BtgI C'CryG_G Cuts at: 513 1065 2004 2793 2889 513 Size: 552 939 789 96 3307 Fragments arranged by size: 3307 939 789 552 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648 Size: 54 1425 50 275 18 1300 889 1629 Cuts at: 5648 8 Size: 43 Fragments arranged by size: 1629 1425 1300 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 2150 3613 1353 Size: 752 45 1463 3423 Fragments arranged by size: 3423 1463 752 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2704 2749 3746 4343 5130 5398 Size: 393 476 596 45 997 597 787 268 Cuts at: 5398 1239 Size: 1524 Fragments arranged by size: 1524 997 787 597 596 476 393 268 45 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2655 2687 2738 2770 4324 Size: 32 135 32 1083 32 51 32 1554 Cuts at: 4324 4356 4398 4430 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1554 1083 135 51 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2228 2459 3641 4000 4159 518 Size: 648 1005 57 231 1182 359 159 2042 Fragments arranged by size: 2042 1182 1005 648 359 231 159 57 MspA1I CmG'CkG Cuts at: 1606 2218 3099 3776 4924 5169 1606 Size: 612 881 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 677 612 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NsiI A_TGCA'T Cuts at: 2028 2028 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2350 2398 3041 4586 1132 Size: 97 1121 48 643 1545 2229 Fragments arranged by size: 2229 1545 1121 643 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2036 2065 2777 3035 830 Size: 1206 29 712 258 3478 Fragments arranged by size: 3478 1206 712 258 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2218 2218 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 2036 3035 2036 Size: 999 4684 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2032 2061 2076 2773 2920 3031 4166 Size: 219 987 29 15 697 147 111 1135 Cuts at: 4166 4847 5038 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 987 697 681 219 191 147 111 29 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 3787 4688 4950 820 Size: 197 926 153 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2038 1517 Size: 521 5162 StyI C'CwwG_G Cuts at: 513 1065 2004 3122 513 Size: 552 939 1118 3074 Fragments arranged by size: 3074 1118 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3710 3727 3880 4065 4510 325 Size: 1255 480 1650 17 153 185 445 1498 Fragments arranged by size: 1650 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 2826 3929 Size: 274 80 33 51 1443 255 594 1103 Cuts at: 3929 5641 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1103 594 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI