##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          177  GTAC                 GT^AC
AlwI          843  GATCC                GGATC(4/5)
AspHI          64  GAGCTC               G[AT]GC[AT]^C
AvrII         255  CCTAGG               C^CTAGG
BanI          624  GGTGCC               G^G[CT][GA]CC
BanII          64  GAGCTC               G[GA]GC[CT]^C
BlnI          255  CCTAGG               C^CTAGG
BpmI           67  CTCCAG               CTGGAG(16/14)
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
BsmAI         646  GTCTC                GTCTC(1/5)
BsrBI         684  CCGCTC               CCGCTC(-3/-3)
BsrDI         603  CATTGC               GCAATG(2/0)
BsrFI         698  GCCGGT               [GA]^CCGG[CT]
BstEII        334  GGTTACC              G^GT[ACGT]ACC
BstPI         334  GGTTACC              G^GT[ACGT]ACC
BstXI         236  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        698  GCCGGT               [GA]^CCGG[CT]
EarI           79  CTCTTC               CTCTTC(1/4)
EcoNI         801  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       334  GGTTACC              G^GT[ACGT]ACC
FauI          607  GCGGG                CCCGC(4/6)
HaeIII        697  GGCC                 GG^CC
HapII         699  CCGG                 C^CGG
HindIII       707  AAGCTT               A^AGCTT
HpaII         699  CCGG                 C^CGG
Ksp632I        79  CTCTTC               CTCTTC(1/4)
MspA1I         70  CAGCTG               C[AC]G^C[GT]G
MspI          699  CCGG                 C^CGG
NdeI          356  CATATG               CA^TATG
NspI          718  ACATGC               [GA]CATG^[CT]
PleI          471  GACTC                GAGTC(4/5)
PvuII          70  CAGCTG               CAG^CTG
RsaI          177  GTAC                 GT^AC
SacI           64  GAGCTC               GAGCT^C
SfaNI         671  GCATC                GCATC(5/9)
TaqI          206  TCGA                 T^CGA
Tsp45I        109  GTCAC                ^GT[GC]AC
Tsp509I        13  AATT                 ^AATT
TthHB8I       206  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          684  CCGC                 CCGC(-3/-1)
              607  GCGG                
BbvCI         186  CCTCAGC              CCTCAGC(-5/-2)
              328  GCTGAGG             
BmyI           64  GAGCTC               G[AGT]GC[ACT]^C
              625  GTGCCC              
BseRI         550  CTCCTC               GAGGAG(10/8)
              687  CTCCTC              
Bsp1286I       64  GAGCTC               G[AGT]GC[ACT]^C
              625  GTGCCC              
Cac8I          57  GCAAGC               GC[ACGT]^[ACGT]GC
              435  GCTGGC              
NcoI            5  CCATGG               C^CATGG
              350  CCATGG              
NlaIV         624  GGTGCC               GG[ACGT]^[ACGT]CC
              757  GGGTCC              
SfcI          378  CTGTAG               C^T[GA][CT]AG
              666  CTACAG              
TfiI           98  GAATC                G^A[AT]TC
              135  GATTC               
TspRI         386  CACTG                CA[GC]TG[ACGT][ACGT]^
              425  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AluI        AvaII       BbvI        BfaI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     Fnu4HI      FokI        HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      Sau3AI      Sau96I      ScrFI       
StyI        Tru9I       TseI