Restriction map for
pFN21AE5928
(Circular) (Six-base) MAPSORT of: pFN21AE5928.seq Check: 4478 from: 1 to: 5638
pFN21AE5928 5638 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 2409
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2208 4537 5514 1203
Size: 1005 2329 977 1327
Fragments arranged by size:
2329 1327 1005 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2402 1166
Size: 1236 4402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2682 2968 3335 2682
Size: 286 367 4985
Fragments arranged by size:
4985 367 286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 2959 4414 618
Size: 325 217 627 443 729 1455 1842
Fragments arranged by size:
1842 1455 729 627 443 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BbvCI CC'TCA_GC
Cuts at: 2578 2578
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2142 1548
Size: 594 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 4249 136
Size: 107 122 71 631 605 760 1817 1525
Fragments arranged by size:
1817 1525 760 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2298 3055 2298
Size: 757 4881
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2428 3699 4855 1790
Size: 445 193 1271 1156 2573
Fragments arranged by size:
2573 1271 1156 445 193
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
Bpu10I CC'TnA_GC
Cuts at: 2390 2578 2390
Size: 188 5450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2104 3429 493
Size: 711 900 1325 2702
Fragments arranged by size:
2702 1325 900 711
BsaBI GATnn'nnATC
Cuts at: 2594 5632 2594
Size: 3038 2600
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2840 3445 3827
Size: 53 83 186 936 537 770 605 382
Cuts at: 3827 4462 275
Size: 635 1451
Fragments arranged by size:
1451 936 770 635 605 537 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2407 2437 1178
Size: 30 165 30 1004 30 4379
Fragments arranged by size:
4379 1004 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2117 1186
Size: 880 51 4707
Fragments arranged by size:
4707 880 51
BseYI C'CCAG_C
Cuts at: 2116 4841 5337 2116
Size: 2725 496 2417
Fragments arranged by size:
2725 2417 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 2589 2906 1163
Size: 573 711 142 317 3895
Fragments arranged by size:
3895 711 573 317 142
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2330 3699 3784 4855 2109
Size: 221 1369 85 1071 2892
Fragments arranged by size:
2892 1369 1071 221 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2235 2330 2428 2957 3699 3784
Size: 399 319 126 95 98 529 742 85
Cuts at: 3784 4855 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 529 399 319 126 98
95 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 2993 844
Size: 1565 584 3489
Fragments arranged by size:
3489 1565 584
BsrBI CCG'CTC
Cuts at: 1394 1823 2389 3525 1394
Size: 429 566 1136 3507
Fragments arranged by size:
3507 1136 566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2438 4282 4469 1700
Size: 110 628 1844 187 2869
Fragments arranged by size:
2869 1844 628 187 110
BsrGI T'GTAC_A
Cuts at: 96 2862 96
Size: 2766 2872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 2021 3698 4710 2021
Size: 1677 1012 2949
Fragments arranged by size:
2949 1677 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 2590
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2520 2682 2968 3335 3720 3737 5189 5275
Size: 548 162 286 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 286 162
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2623 2623
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2793 2802 513
Size: 552 1728 9 3349
Fragments arranged by size:
3349 1728 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2254 4645 809
Size: 507 938 2391 1802
Fragments arranged by size:
2391 1802 938 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2433 2817
Size: 54 1425 50 275 18 543 60 384
Cuts at: 2817 3085 3974 5603 8
Size: 268 889 1629 43
Fragments arranged by size:
1629 1425 889 543 384 275 268 60
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3701 4298 5085 5353 1239
Size: 393 2069 597 787 268 1524
Fragments arranged by size:
2069 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4752 4936 1305
Size: 729 2718 184 2007
Fragments arranged by size:
2718 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 2435 2819 10
Size: 54 1425 343 543 60 384 2829
Fragments arranged by size:
2829 1425 543 384 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2207 2351 2402 2600 3596 3955
Size: 648 1005 36 144 51 198 996 359
Cuts at: 3955 4114 518
Size: 159 2042
Fragments arranged by size:
2042 1005 996 648 359 198 159 144
51 36
MspA1I CmG'CkG
Cuts at: 1606 2289 3054 3731 4879 5124 1606
Size: 683 765 677 1148 245 2120
Fragments arranged by size:
2120 1148 765 683 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2793 2802 513
Size: 552 1728 9 3349
Fragments arranged by size:
3349 1728 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2212 2996 4541 1132
Size: 97 983 784 1545 2229
Fragments arranged by size:
2229 1545 983 784 97
PasI CC'CwG_GG
Cuts at: 1348 2060 1348
Size: 712 4926
PciI A'CATG_T
Cuts at: 2208 4537 2208
Size: 2329 3309
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4426
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2990 830
Size: 2160 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2289 2289
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2986 4121 4802 4993 826
Size: 219 1941 1135 681 191 1471
Fragments arranged by size:
1941 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2046 1517
Size: 529 5109
StyI C'CwwG_G
Cuts at: 513 1065 2250 2793 2802 3077 513
Size: 552 1185 543 9 275 3074
Fragments arranged by size:
3074 1185 552 543 275 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2862 3884
Size: 274 80 33 51 1443 198 687 1022
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1022 687 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 2407 2439 2535 2567 5598 5630 2032
Size: 32 343 32 96 32 3031 32 2040
Fragments arranged by size:
3031 2040 343 96 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2557 2800 2557
Size: 243 5395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI