Restriction map for
pFN21AE5481
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BbeI BbrPI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tsp509I Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwNI 842 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 851 GGGCCC GGGCC^C
AseI 622 ATTAAT AT^TAAT
AsnI 622 ATTAAT AT^TAAT
AspHI 784 GTGCTC G[AT]GC[AT]^C
AviII 719 TGCGCA TGC^GCA
BbsI 862 GTCTTC GAAGAC(2/6)
BbvCI 201 CCTCAGC CCTCAGC(-5/-2)
BcgI 647 GCATTGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 862 GTCTTC GAAGAC(2/6)
BsaAI 796 TACGTG [CT]AC^GT[GA]
BsiHKAI 784 GTGCTC G[AT]GC[AT]^C
BspMI 818 ACCTGC ACCTGC(4/8)
BsrBI 9 CCGCTC CCGCTC(-3/-3)
BsrDI 561 CATTGC GCAATG(2/0)
BsrFI 740 GCCGGC [GA]^CCGG[CT]
CfoI 720 GCGC GCG^C
Cfr10I 740 GCCGGC [GA]^CCGG[CT]
DpnII 315 GATC ^GATC
DraIII 369 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 275 CCAAGG C^C[AT][AT]GG
FauI 747 GCGGG CCCGC(4/6)
FspI 719 TGCGCA TGC^GCA
HapII 741 CCGG C^CGG
HhaI 720 GCGC GCG^C
HinP1I 720 GCGC G^CGC
HincII 422 GTCAAC GT[CT]^[GA]AC
HindII 422 GTCAAC GT[CT]^[GA]AC
HpaII 741 CCGG C^CGG
MaeII 797 ACGT A^CGT
MboI 315 GATC ^GATC
MseI 623 TTAA T^TAA
MspI 741 CCGG C^CGG
NaeI 740 GCCGGC GCC^GGC
NdeII 315 GATC ^GATC
NgoMIV 740 GCCGGC G^CCGGC
NspI 930 GCATGC [GA]CATG^[CT]
PshBI 622 ATTAAT AT^TAAT
PvuII 710 CAGCTG CAG^CTG
Sau3AI 315 GATC ^GATC
SphI 930 GCATGC GCATG^C
StuI 288 AGGCCT AGG^CCT
StyI 275 CCAAGG C^C[AT][AT]GG
Tru9I 623 TTAA T^TAA
Tsp45I 265 GTGAC ^GT[GC]AC
XcmI 50 CCAACCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 258 GTCTAC GT^[AC][GT]AC
969 GTCTAC
BalI 362 TGGCCA TGG^CCA
443 TGGCCA
BanI 230 GGCACC G^G[CT][GA]CC
496 GGTGCC
BanII 186 GGGCTC G[GA]GC[CT]^C
851 GGGCCC
BpmI 831 CTCCAG CTGGAG(16/14)
79 CTGGAG
Bsp1407I 383 TGTACA T^GTACA
607 TGTACA
BsrGI 383 TGTACA T^GTACA
607 TGTACA
BstAPI 368 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
930 GCATGCTCTGC
BstXI 275 CCAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
771 CCAGCTCACTGG
DdeI 202 CTCAG C^T[ACGT]AG
371 CTGAG
DraII 850 GGGGCCC [GA]G^G[ACGT]CC[CT]
851 GGGCCCC
EaeI 362 TGGCCA [CT]^GGCC[GA]
443 TGGCCA
EarI 115 CTCTTC CTCTTC(1/4)
127 CTCTTC
EcoO109I 850 GGGGCCC [GA]G^G[ACGT]CC[CT]
851 GGGCCCC
Ksp632I 115 CTCTTC CTCTTC(1/4)
127 CTCTTC
MaeIII 265 GTGAC ^GT[ACGT]AC
877 GTAAC
MluNI 362 TGGCCA TGG^CCA
443 TGGCCA
MscI 362 TGGCCA TGG^CCA
443 TGGCCA
MspA1I 710 CAGCTG C[AC]G^C[GT]G
745 CAGCGG
PstI 662 CTGCAG CTGCA^G
820 CTGCAG
SspBI 383 TGTACA T^GTACA
607 TGTACA
TfiI 162 GAATC G^A[AT]TC
479 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BmyI BsaJI BseRI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboII MnlI MslI MvaI
MwoI NlaIII NlaIV PflMI PleI
RsaI Sau96I ScrFI SfaNI SfcI
TseI TspRI Van91I