(Circular) (Six-base) MAPSORT of: pFN21AE5481.seq Check: 7986 from: 1 to: 5698 pFN21AE5481 5698 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2264 2975 3041 1937 Size: 327 711 66 4594 Fragments arranged by size: 4594 711 327 66 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 2786 1166 Size: 1620 4078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2852 3441 5013 2852 Size: 589 1572 3537 Fragments arranged by size: 3537 1572 589 ApaI G_GGCC'C Cuts at: 2860 2860 Size: 5698 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 2628 4252 160 Size: 872 1596 1624 1606 Fragments arranged by size: 1624 1606 1596 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2204 2237 2204 Size: 33 5665 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 2501 3019 4474 618 Size: 325 217 627 448 266 518 1455 1842 Fragments arranged by size: 1842 1455 627 518 448 325 266 217 BanII G_rGCy'C Cuts at: 1391 2195 2860 3017 1391 Size: 804 665 157 4072 Fragments arranged by size: 4072 804 665 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2860 928 Size: 971 961 3766 Fragments arranged by size: 3766 971 961 BbvCI CC'TCA_GC Cuts at: 2207 2207 Size: 5698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2641 2675 3889 3923 1903 Size: 34 704 34 1214 34 3678 Fragments arranged by size: 3678 1214 704 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 2238 2280 2860 3759 4915 1790 Size: 448 42 580 899 1156 2573 Fragments arranged by size: 2573 1156 899 580 448 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2438 2793 2990 Size: 416 53 83 399 24 1021 355 197 Cuts at: 2990 3429 4387 442 Size: 439 958 1753 Fragments arranged by size: 1753 1021 958 439 416 399 355 197 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2821 4358 1239 Size: 866 716 1537 2579 Fragments arranged by size: 2579 1537 866 716 Bpu10I CC'TnA_GC Cuts at: 2207 2207 Size: 5698 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2803 3489 493 Size: 711 1599 686 2702 Fragments arranged by size: 2702 1599 711 686 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2129 2162 1186 Size: 934 9 33 4722 Fragments arranged by size: 4722 934 33 9 BseYI C'CCAG_C Cuts at: 2162 2775 2852 4901 5397 2162 Size: 613 77 2049 496 2463 Fragments arranged by size: 2463 2049 613 496 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2490 2752 2977 1163 Size: 573 684 70 262 225 3884 Fragments arranged by size: 3884 684 573 262 225 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2793 3759 3844 4915 2793 Size: 966 85 1071 3576 Fragments arranged by size: 3576 1071 966 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2238 2280 2793 2860 3017 3759 Size: 399 405 43 42 513 67 157 742 Cuts at: 3759 3844 4915 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 405 399 157 85 67 43 42 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2832 3053 844 Size: 1988 221 3489 Fragments arranged by size: 3489 1988 221 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3585 1394 Size: 429 193 1569 3507 Fragments arranged by size: 3507 1569 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 4193 4367 65 Size: 1183 1317 1628 174 1396 Fragments arranged by size: 1628 1396 1317 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2745 4342 4529 1700 Size: 110 935 1597 187 2869 Fragments arranged by size: 2869 1597 935 187 110 BsrGI T'GTAC_A Cuts at: 96 2388 2612 96 Size: 2292 224 3182 Fragments arranged by size: 3182 2292 224 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2379 2941 794 Size: 1585 562 3551 Fragments arranged by size: 3551 1585 562 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2783 2287 Size: 496 5202 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2769 3214 4008 4028 2769 Size: 445 794 20 4439 Fragments arranged by size: 4439 794 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DraIII CAC_nnn'GTG Cuts at: 2379 2379 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 2448 3145 Size: 54 1425 50 275 18 537 81 697 Cuts at: 3145 4034 5663 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 697 537 275 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2138 3628 1353 Size: 773 12 1490 3423 Fragments arranged by size: 3423 1490 773 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2528 4285 4659 4805 387 Size: 1064 138 939 1757 374 146 1280 Fragments arranged by size: 1757 1280 1064 939 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2821 3761 4358 5145 5413 1239 Size: 393 473 716 940 597 787 268 1524 Fragments arranged by size: 1524 940 787 716 597 473 393 268 EcoO109I rG'GnC_Cy Cuts at: 2856 2857 2856 Size: 1 5697 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 2726 4204 2726 Size: 1478 4220 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373 Size: 32 135 32 2767 32 42 32 2626 Fragments arranged by size: 2767 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2429 3042 3288 669 Size: 988 281 491 613 246 3079 Fragments arranged by size: 3079 988 613 491 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2450 10 Size: 54 1425 343 537 81 3258 Fragments arranged by size: 3258 1425 537 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2396 2786 3656 4015 4174 518 Size: 648 990 240 390 870 359 159 2042 Fragments arranged by size: 2042 990 870 648 390 359 240 159 MspA1I CmG'CkG Cuts at: 1606 2717 2752 3114 3791 4939 5184 1606 Size: 1111 35 362 677 1148 245 2120 Fragments arranged by size: 2120 1148 1111 677 362 245 35 NaeI GCC'GGC Cuts at: 2747 2747 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NgoMIV G'CCGG_C Cuts at: 2745 2745 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2939 3056 4601 1132 Size: 97 1710 117 1545 2229 Fragments arranged by size: 2229 1710 1545 117 97 PasI CC'CwG_GG Cuts at: 1348 2740 1348 Size: 1392 4306 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 2230 2365 1108 Size: 957 165 135 4441 Fragments arranged by size: 4441 957 165 135 PfoI T'CCnGG_A Cuts at: 2082 2247 2082 Size: 165 5533 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2856 2856 Size: 5698 PstI C_TGCA'G Cuts at: 830 2671 2829 3050 830 Size: 1841 158 221 3478 Fragments arranged by size: 3478 1841 221 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2717 2717 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2146 2265 2667 2825 2976 3046 4181 Size: 219 1101 119 402 158 151 70 1135 Cuts at: 4181 4862 5053 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1101 681 402 219 191 158 151 119 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 2939 3056 1132 Size: 1807 117 3774 Fragments arranged by size: 3774 1807 117 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 2295 1517 Size: 778 4920 StyI C'CwwG_G Cuts at: 513 1065 2280 3137 513 Size: 552 1215 857 3074 Fragments arranged by size: 3074 1215 857 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 2612 2796 Size: 274 80 33 51 1443 411 224 184 Cuts at: 2796 3944 5656 96 Size: 1148 1712 138 Fragments arranged by size: 1712 1443 1148 411 274 224 184 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2062 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI