(Linear) (Six-base) MAPSORT of: KIEE5481.seq Check: 820 from: 1 to: 993 KIEE5481.orf With 182 enzymes: SgfI * January 29, 2014 12:02 .. AccI GT'mk_AC Cuts at: 0 259 970 993 Size: 259 711 23 Fragments arranged by size: 711 259 23 AleI CACnn'nnGTG Cuts at: 0 781 993 Size: 781 212 AlwNI CAG_nnn'CTG Cuts at: 0 847 993 Size: 847 146 ApaI G_GGCC'C Cuts at: 0 855 993 Size: 855 138 AseI AT'TA_AT Cuts at: 0 623 993 Size: 623 370 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 199 232 993 Size: 199 33 761 Fragments arranged by size: 761 199 33 BanI G'GyrC_C Cuts at: 0 230 496 993 Size: 230 266 497 Fragments arranged by size: 497 266 230 BanII G_rGCy'C Cuts at: 0 190 855 993 Size: 190 665 138 Fragments arranged by size: 665 190 138 BbsI GAAGACnn'nnnn_ Cuts at: 0 855 993 Size: 855 138 BbvCI CC'TCA_GC Cuts at: 0 202 993 Size: 202 791 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 636 670 993 Size: 636 34 323 Fragments arranged by size: 636 323 34 Bme1580I G_kGCm'C Cuts at: 0 233 275 855 993 Size: 233 42 580 138 Fragments arranged by size: 580 233 138 42 BmrI ACTGGGnnnn_n' Cuts at: 0 433 788 993 Size: 433 355 205 Fragments arranged by size: 433 355 205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 816 993 Size: 100 716 177 Fragments arranged by size: 716 177 100 Bpu10I CC'TnA_GC Cuts at: 0 202 993 Size: 202 791 BsaAI yAC'GTr Cuts at: 0 798 993 Size: 798 195 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 124 157 993 Size: 115 9 33 836 Fragments arranged by size: 836 115 33 9 BseYI C'CCAG_C Cuts at: 0 157 770 847 993 Size: 157 613 77 146 Fragments arranged by size: 613 157 146 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 485 747 972 993 Size: 415 70 262 225 21 Fragments arranged by size: 415 262 225 70 21 BsiHKAI G_wGCw'C Cuts at: 0 788 993 Size: 788 205 Bsp1286I G_dGCh'C Cuts at: 0 190 233 275 788 855 993 Size: 190 43 42 513 67 138 Fragments arranged by size: 513 190 138 67 43 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 827 993 Size: 827 166 BsrBI CCG'CTC Cuts at: 0 11 993 Size: 11 982 BsrDI GCAATG_nn' Cuts at: 0 560 993 Size: 560 433 BsrFI r'CCGG_y Cuts at: 0 740 993 Size: 740 253 BsrGI T'GTAC_A Cuts at: 0 383 607 993 Size: 383 224 386 Fragments arranged by size: 386 383 224 BstAPI GCAn_nnn'nTGC Cuts at: 0 374 936 993 Size: 374 562 57 Fragments arranged by size: 562 374 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 282 778 993 Size: 282 496 215 Fragments arranged by size: 496 282 215 BtsI GCAGTG_nn' Cuts at: 0 764 993 Size: 764 229 DraIII CAC_nnn'GTG Cuts at: 0 374 993 Size: 374 619 EaeI y'GGCC_r Cuts at: 0 362 443 993 Size: 362 81 550 Fragments arranged by size: 550 362 81 EarI CTCTTCn'nnn_ Cuts at: 0 121 133 993 Size: 121 12 860 Fragments arranged by size: 860 121 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 523 993 Size: 523 470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 816 993 Size: 100 716 177 Fragments arranged by size: 716 177 100 EcoO109I rG'GnC_Cy Cuts at: 0 851 852 993 Size: 851 1 141 Fragments arranged by size: 851 141 1 FspI TGC'GCA Cuts at: 0 721 993 Size: 721 272 HincII GTy'rAC Cuts at: 0 424 993 Size: 424 569 MscI TGG'CCA Cuts at: 0 364 445 993 Size: 364 81 548 Fragments arranged by size: 548 364 81 MslI CAynn'nnrTG Cuts at: 0 151 391 781 993 Size: 151 240 390 212 Fragments arranged by size: 390 240 212 151 MspA1I CmG'CkG Cuts at: 0 712 747 993 Size: 712 35 246 Fragments arranged by size: 712 246 35 NaeI GCC'GGC Cuts at: 0 742 993 Size: 742 251 NgoMIV G'CCGG_C Cuts at: 0 740 993 Size: 740 253 NspI r_CATG'y Cuts at: 0 934 993 Size: 934 59 PasI CC'CwG_GG Cuts at: 0 735 993 Size: 735 258 PflMI CCAn_nnn'nTGG Cuts at: 0 60 225 360 993 Size: 60 165 135 633 Fragments arranged by size: 633 165 135 60 PfoI T'CCnGG_A Cuts at: 0 77 242 993 Size: 77 165 751 Fragments arranged by size: 751 165 77 PspOMI G'GGCC_C Cuts at: 0 851 993 Size: 851 142 PstI C_TGCA'G Cuts at: 0 666 824 993 Size: 666 158 169 Fragments arranged by size: 666 169 158 PvuII CAG'CTG Cuts at: 0 712 993 Size: 712 281 SfcI C'TryA_G Cuts at: 0 141 260 662 820 971 993 Size: 141 119 402 158 151 22 Fragments arranged by size: 402 158 151 141 119 22 SphI G_CATG'C Cuts at: 0 934 993 Size: 934 59 StuI AGG'CCT Cuts at: 0 290 993 Size: 290 703 StyI C'CwwG_G Cuts at: 0 275 993 Size: 275 718 TatI w'GTAC_w Cuts at: 0 383 607 791 993 Size: 383 224 184 202 Fragments arranged by size: 383 224 202 184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 57 993 Size: 57 936 Enzymes that do cut: AccI AleI AlwNI ApaI AseI BaeI BanI BanII BbsI BbvCI BcgI Bme1580I BmrI BpmI Bpu10I BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FspI HincII MscI MslI MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PspOMI PstI PvuII SfcI SphI StuI StyI TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AsiSI AvaI AvrII BamHI BbeI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI