(Circular) (Six-base) MAPSORT of: pFN21AE6046.seq Check: 8523 from: 1 to: 5659 pFN21AE6046 5659 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2175 2175 Size: 5659 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 3002 1937 Size: 780 285 4594 Fragments arranged by size: 4594 780 285 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3722 5106 5374 1632 Size: 2090 1384 268 1917 Fragments arranged by size: 2090 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 2956 4558 5535 1203 Size: 1753 1602 977 1327 Fragments arranged by size: 1753 1602 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2392 3402 4974 2392 Size: 1010 1572 3077 Fragments arranged by size: 3077 1572 1010 ApaLI G'TGCA_C Cuts at: 2427 3716 4872 2427 Size: 1289 1156 3214 Fragments arranged by size: 3214 1289 1156 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 AvrII C'CTAG_G Cuts at: 2240 2240 Size: 5659 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2568 2601 2568 Size: 33 5626 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 4435 618 Size: 325 217 627 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 BanII G_rGCy'C Cuts at: 1391 2055 2978 1391 Size: 664 923 4072 Fragments arranged by size: 4072 923 664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BbvCI CC'TCA_GC Cuts at: 2550 2550 Size: 5659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2556 2886 1548 Size: 1008 330 4321 Fragments arranged by size: 4321 1008 330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 5654 2337 Size: 3317 2342 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2431 3720 4876 1790 Size: 641 1289 1156 2573 Fragments arranged by size: 2573 1289 1156 641 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 3390 4348 Size: 416 53 83 399 24 640 1333 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1333 958 640 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2960 4319 1239 Size: 1721 1359 2579 Fragments arranged by size: 2579 1721 1359 Bpu10I CC'TnA_GC Cuts at: 2550 2550 Size: 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 1178 Size: 30 165 30 657 30 4747 Fragments arranged by size: 4747 657 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2926 1186 Size: 1740 3919 BseYI C'CCAG_C Cuts at: 2385 4862 5358 2385 Size: 2477 496 2686 Fragments arranged by size: 2686 2477 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2378 2554 1163 Size: 573 417 225 176 4268 Fragments arranged by size: 4268 573 417 225 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 2946 3720 3805 4876 2431 Size: 515 774 85 1071 3214 Fragments arranged by size: 3214 1071 774 515 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 BsmBI CGTCTCn'nnnn_ Cuts at: 2821 2821 Size: 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2431 2946 2978 3720 3805 4876 Size: 399 265 376 515 32 742 85 1071 Cuts at: 4876 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 515 399 376 265 85 32 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 3014 844 Size: 1331 839 3489 Fragments arranged by size: 3489 1331 839 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2373 2733 4154 4328 65 Size: 1183 1125 360 1421 174 1396 Fragments arranged by size: 1421 1396 1183 1125 360 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2620 2743 1184 Size: 1436 123 4100 Fragments arranged by size: 4100 1436 123 BstXI CCAn_nnnn'nTGG Cuts at: 2324 2324 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2337 2989 3356 3741 3758 5210 5296 5308 Size: 365 652 367 385 17 1452 86 12 Cuts at: 5308 5654 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 652 385 367 365 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2802 513 Size: 552 1737 3370 Fragments arranged by size: 3370 1737 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2663 3175 3969 3989 2663 Size: 512 794 20 4333 Fragments arranged by size: 4333 794 512 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2813 4666 809 Size: 507 1497 1853 1802 Fragments arranged by size: 1853 1802 1497 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2892 3106 3995 Size: 54 1425 50 275 18 1062 214 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1062 889 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2230 2312 3589 1353 Size: 877 82 1277 3423 Fragments arranged by size: 3423 1277 877 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2960 3722 4319 5106 5374 1239 Size: 393 1328 762 597 787 268 1524 Fragments arranged by size: 1524 1328 787 762 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2710 2923 2710 Size: 213 5446 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 4300 4332 4374 Size: 32 135 32 674 32 2022 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 2022 674 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 3003 3249 669 Size: 988 281 316 749 246 3079 Fragments arranged by size: 3079 988 749 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 2832 3258 2832 Size: 426 5233 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 4773 4957 1305 Size: 876 2592 184 2007 Fragments arranged by size: 2592 2007 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2894 10 Size: 54 1425 343 1062 2775 Fragments arranged by size: 2775 1425 1062 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2738 2843 2894 3617 3976 4135 Size: 648 1011 561 105 51 723 359 159 Cuts at: 4135 518 Size: 2042 Fragments arranged by size: 2042 1011 723 648 561 359 159 105 51 MspA1I CmG'CkG Cuts at: 1606 2500 2554 3075 3752 4900 5145 1606 Size: 894 54 521 677 1148 245 2120 Fragments arranged by size: 2120 1148 894 677 521 245 54 NcoI C'CATG_G Cuts at: 513 1065 2802 513 Size: 552 1737 3370 Fragments arranged by size: 3370 1737 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2960 3017 4562 1132 Size: 97 1731 57 1545 2229 Fragments arranged by size: 2229 1731 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 2956 4558 2956 Size: 1602 4057 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 1108 Size: 1402 4257 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2923 2923 Size: 5659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2500 2554 2500 Size: 54 5605 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2118 3007 4142 4823 5014 826 Size: 219 1073 889 1135 681 191 1471 Fragments arranged by size: 1471 1135 1073 889 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2785 1517 Size: 1268 4391 StyI C'CwwG_G Cuts at: 513 1065 2240 2802 3098 513 Size: 552 1175 562 296 3074 Fragments arranged by size: 3074 1175 562 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 3686 3703 3856 4041 4486 325 Size: 1255 860 1246 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1246 860 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2476 3905 5617 Size: 274 80 33 51 1443 499 1429 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1443 1429 499 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI