################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 796 CGCG CG^CG AccIII 629 TCCGGA T^CCGGA AlwNI 372 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 455 GGGCCC GGGCC^C Asp700I 560 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 415 GAAGAC GAAGAC(2/6) BbvCI 403 GCTGAGG CCTCAGC(-5/-2) BglII 593 AGATCT A^GATCT BpuAI 415 GAAGAC GAAGAC(2/6) BsaWI 629 TCCGGA [AT]^CCGG[AT] BseAI 629 TCCGGA T^CCGGA BspEI 629 TCCGGA T^CCGGA BsrI 321 CCAGT ACTGG(1/-1) BstUI 796 CGCG CG^CG BstYI 593 AGATCT [GA]^GATC[CT] CfoI 797 GCGC GCG^C EcoT14I 800 CCTTGG C^C[AT][AT]GG HhaI 797 GCGC GCG^C HinP1I 797 GCGC G^CGC HincII 756 GTTGAC GT[CT]^[GA]AC HindII 756 GTTGAC GT[CT]^[GA]AC MaeIII 665 GTCAC ^GT[ACGT]AC MflI 593 AGATCT [GA]^GATC[CT] MroI 629 TCCGGA T^CCGGA MspA1I 285 CAGCTG C[AC]G^C[GT]G MvnI 796 CGCG CG^CG NspI 710 GCATGC [GA]CATG^[CT] PleI 468 GAGTC GAGTC(4/5) PpuMI 230 AGGACCT [GA]G^G[AT]CC[CT] PvuII 285 CAGCTG CAG^CTG SfcI 597 CTACAG C^T[GA][CT]AG SphI 710 GCATGC GCATG^C StyI 800 CCTTGG C^C[AT][AT]GG TfiI 310 GAATC G^A[AT]TC Tsp45I 665 GTCAC ^GT[GC]AC XhoII 593 AGATCT [GA]^GATC[CT] XmnI 560 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 10 GAGCTC G[AT]GC[AT]^C 382 GAGCTC BbvI 491 GCAGC GCAGC(8/12) 287 GCTGC BciVI 186 GTATCC GTATCC(6/5) 824 GTATCC BcnI 515 CCCGG CC^[GC]GG 639 CCCGG BfaI 106 CTAG C^TAG 817 CTAG BsaI 207 GGTCTC GGTCTC(1/5) 338 GGTCTC BsiHKAI 10 GAGCTC G[AT]GC[AT]^C 382 GAGCTC EarI 222 GAAGAG CTCTTC(1/4) 391 GAAGAG HinfI 310 GAATC G^A[ACGT]TC 468 GAGTC HphI 26 TCACC GGTGA(8/7) 36 TCACC Ksp632I 222 GAAGAG CTCTTC(1/4) 391 GAAGAG MaeI 106 CTAG C^TAG 817 CTAG MaeII 216 ACGT A^CGT 584 ACGT MseI 86 TTAA T^TAA 556 TTAA NciI 515 CCCGG CC^[GC]GG 639 CCCGG NlaIII 711 CATG CATG^ 837 CATG PflMI 152 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 371 CCAGCACCTGG SacI 10 GAGCTC GAGCT^C 382 GAGCTC SfaNI 280 GATGC GCATC(5/9) 855 GATGC Tru9I 86 TTAA T^TAA 556 TTAA TseI 287 GCTGC G^C[AT]GC 491 GCAGC TspRI 314 CACTG CA[GC]TG[ACGT][ACGT]^ 832 CAGTG Van91I 152 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 371 CCAGCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AvaII BanII BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TaqI Tsp509I TthHB8I