################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MluI MseI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 572 TCCGGA T^CCGGA AcsI 256 GAATTT [GA]^AATT[CT] AocI 339 CCTGAGG CC^T[ACGT]AGG ApoI 256 GAATTT [GA]^AATT[CT] AvaII 588 GGACC G^G[AT]CC BalI 389 TGGCCA TGG^CCA BanII 565 GAGCCC G[GA]GC[CT]^C BciVI 153 GTATCC GTATCC(6/5) BsaBI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 470 CCAAGG C^C[ACGT][ACGT]GG BsaWI 572 TCCGGA [AT]^CCGG[AT] BseAI 572 TCCGGA T^CCGGA BseRI 491 GAGGAG GAGGAG(10/8) BsmBI 612 GAGACG CGTCTC(1/5) BspEI 572 TCCGGA T^CCGGA BsrBI 494 GAGCGG CCGCTC(-3/-3) BssKI 157 CCTGG ^CC[ACGT]GG BssSI 488 CACGAG CACGAG(-5/-1) BstNI 157 CCTGG CC^[AT]GG BstYI 160 GGATCT [GA]^GATC[CT] Bsu36I 339 CCTGAGG CC^T[ACGT]AGG DraII 587 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 389 TGGCCA [CT]^GGCC[GA] Eco81I 339 CCTGAGG CC^T[ACGT]AGG EcoO109I 587 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 157 CCTGG ^CC[AT]GG EcoT14I 470 CCAAGG C^C[AT][AT]GG HapII 573 CCGG C^CGG HgaI 614 GACGC GACGC(5/10) HinfI 606 GAATC G^A[ACGT]TC HpaII 573 CCGG C^CGG MamI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 395 CAATTG C^AATTG MflI 160 GGATCT [GA]^GATC[CT] MluNI 389 TGGCCA TGG^CCA MroI 572 TCCGGA T^CCGGA MscI 389 TGGCCA TGG^CCA MslI 366 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 583 CAGCGG C[AC]G^C[GT]G MspI 573 CCGG C^CGG MunI 395 CAATTG C^AATTG MvaI 157 CCTGG CC^[AT]GG NspI 142 ACATGC [GA]CATG^[CT] PpuMI 587 GGGACCT [GA]G^G[AT]CC[CT] ScrFI 157 CCTGG CC^[ACGT]GG SspI 135 AATATT AAT^ATT StyI 470 CCAAGG C^C[AT][AT]GG TfiI 606 GAATC G^A[AT]TC XhoII 160 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 160 GGATC GGATC(4/5) 237 GGATC BbvI 432 GCTGC GCAGC(8/12) 435 GCTGC BmyI 9 GGGCAC G[AGT]GC[ACT]^C 565 GAGCCC BpmI 18 CTGGAG CTGGAG(16/14) 450 CTGGAG BsmAI 612 GAGAC GTCTC(1/5) 112 GTCTC BsmFI 519 GGGAC GGGAC(10/14) 587 GGGAC Bsp1286I 9 GGGCAC G[AGT]GC[ACT]^C 565 GAGCCC Cfr13I 30 GGGCC G^G[ACGT]CC 588 GGACC DdeI 149 CTCAG C^T[ACGT]AG 340 CTGAG DpnII 161 GATC ^GATC 238 GATC FauI 496 GCGGG CCCGC(4/6) 585 GCGGG HphI 202 GGTGA GGTGA(8/7) 240 TCACC MaeIII 246 GTCAC ^GT[ACGT]AC 484 GTGAC MboI 161 GATC ^GATC 238 GATC MboII 76 GAAGA GAAGA(8/7) 316 GAAGA NdeII 161 GATC ^GATC 238 GATC NlaIII 143 CATG CATG^ 366 CATG NlaIV 124 GGAACC GG[ACGT]^[ACGT]CC 587 GGGACC Sau3AI 161 GATC ^GATC 238 GATC Sau96I 30 GGGCC G^G[ACGT]CC 588 GGACC SfaNI 319 GATGC GCATC(5/9) 617 GCATC TseI 432 GCTGC G^C[AT]GC 435 GCTGC Tsp45I 246 GTCAC ^GT[GC]AC 484 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AciI BsrI BstF5I Cac8I Fnu4HI FokI HaeIII ItaI MnlI Tsp509I TspRI