(Linear) (Six-base) MAPSORT of: KIEE3054.seq  Check: 7741  from: 1  to: 621



KIEE3054.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     621 
   Size:        271     350
 
ApoI r'AATT_y

Cuts at:      0     256     621 
   Size:        256     365
 
BanII G_rGCy'C

Cuts at:      0     569     621 
   Size:        569      52
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     164     621 
   Size:        164     457
 
Bme1580I G_kGCm'C

Cuts at:      0      13     621 
   Size:         13     608
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     431     621 
   Size:        431     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     471     621 
   Size:         39     432     150

  Fragments arranged by size: 

                 432     150      39
 
BsaBI GATnn'nnATC

Cuts at:      0     236     621 
   Size:        236     385
 
BsaWI w'CCGG_w

Cuts at:      0     572     621 
   Size:        572      49
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     506     621 
   Size:        506     115
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     606     621 
   Size:        606      15
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     569     621 
   Size:         13     556      52

  Fragments arranged by size: 

                 556      52      13
 
BspEI T'CCGG_A

Cuts at:      0     572     621 
   Size:        572      49
 
BsrBI CCG'CTC

Cuts at:      0     496     621 
   Size:        496     125
 
BssSI C'ACGA_G

Cuts at:      0     488     621 
   Size:        488     133
 
BstYI r'GATC_y

Cuts at:      0     160     621 
   Size:        160     461
 
Bsu36I CC'TnA_GG

Cuts at:      0     340     621 
   Size:        340     281
 
BtsI GCAGTG_nn'

Cuts at:      0     234     621 
   Size:        234     387
 
EaeI y'GGCC_r

Cuts at:      0     389     621 
   Size:        389     232
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     271     471     621 
   Size:         39     232     200     150

  Fragments arranged by size: 

                 232     200     150      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     588     621 
   Size:        588      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     132     164     229     261     367     399     621 
   Size:        132      32      65      32     106      32     222

  Fragments arranged by size: 

                 222     132     106      65      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     395     621 
   Size:        395     226
 
MscI TGG'CCA

Cuts at:      0     391     621 
   Size:        391     230
 
MslI CAynn'nnrTG

Cuts at:      0     370     621 
   Size:        370     251
 
MspA1I CmG'CkG

Cuts at:      0     585     621 
   Size:        585      36
 
NspI r_CATG'y

Cuts at:      0     146     621 
   Size:        146     475
 
PfoI T'CCnGG_A

Cuts at:      0     156     621 
   Size:        156     465
 
PpuMI rG'GwC_Cy

Cuts at:      0     588     621 
   Size:        588      33
 
SspI AAT'ATT

Cuts at:      0     137     621 
   Size:        137     484
 
StyI C'CwwG_G

Cuts at:      0     470     621 
   Size:        470     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      73     621 
   Size:         73     548
 

 Enzymes that do cut:

     AcuI     ApoI    BanII    BciVI Bme1580I     BmrI     BpmI    BsaBI
    BsaWI    BseRI    BsmBI Bsp1286I    BspEI    BsrBI    BssSI    BstYI
   Bsu36I     BtsI     EaeI  Eco57MI EcoO109I    Hin4I     MfeI     MscI
     MslI   MspA1I     NspI     PfoI    PpuMI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI