Restriction map for
pFN21AE5534
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BbvCI BfaI
BfrI BglI BglII BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1252 GTATAC GT^[AC][GT]AC
AcsI 838 GAATTC [GA]^AATT[CT]
AcyI 1035 GGCGTC G[GA]^CG[CT]C
ApoI 838 GAATTC [GA]^AATT[CT]
Asp700I 363 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 56 TGCGCA TGC^GCA
BalI 818 TGGCCA TGG^CCA
BbsI 600 GAAGAC GAAGAC(2/6)
BcgI 291 CGAAGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 68 GTATCC GTATCC(6/5)
BclI 21 TGATCA T^GATCA
BcnI 1125 CCCGG CC^[GC]GG
BlpI 1303 GCTGAGC GC^T[ACGT]AGC
BpmI 956 CTCCAG CTGGAG(16/14)
Bpu1102I 1303 GCTGAGC GC^T[ACGT]AGC
BpuAI 600 GAAGAC GAAGAC(2/6)
BsaHI 1035 GGCGTC G[GA]^CG[CT]C
BsmFI 248 GTCCC GGGAC(10/14)
BspHI 878 TCATGA T^CATGA
BsrDI 1067 GCAATG GCAATG(2/0)
Bst1107I 1252 GTATAC GTA^TAC
BstZ17I 1252 GTATAC GTA^TAC
CelII 1303 GCTGAGC GC^T[ACGT]AGC
CfoI 57 GCGC GCG^C
DraIII 1059 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 74 CCATGG C^C[GA][CT]GG
EarI 591 GAAGAG CTCTTC(1/4)
EcoRI 838 GAATTC G^AATTC
FbaI 21 TGATCA T^GATCA
FspI 56 TGCGCA TGC^GCA
HapII 1126 CCGG C^CGG
HgaI 1036 GCGTC GACGC(5/10)
HhaI 57 GCGC GCG^C
Hin1I 1035 GGCGTC G[GA]^CG[CT]C
HinP1I 57 GCGC G^CGC
HpaII 1126 CCGG C^CGG
Ksp632I 591 GAAGAG CTCTTC(1/4)
MaeII 683 ACGT A^CGT
MluNI 818 TGGCCA TGG^CCA
MscI 818 TGGCCA TGG^CCA
MspA1I 223 CAGCTG C[AC]G^C[GT]G
MspI 1126 CCGG C^CGG
NciI 1125 CCCGG CC^[GC]GG
NcoI 74 CCATGG C^CATGG
PflMI 477 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 349 GACTC GAGTC(4/5)
PpuMI 716 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 223 CAGCTG CAG^CTG
RcaI 878 TCATGA T^CATGA
SacI 949 GAGCTC GAGCT^C
SapI 591 GAAGAGC GCTCTTC(1/4)
SmlI 534 CTCAAG C^T[CT][GA]AG
SphI 524 GCATGC GCATG^C
StuI 629 AGGCCT AGG^CCT
Tsp509I 839 AATT ^AATT
Van91I 477 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1149 CCAGGGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 363 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwNI 340 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
478 CAGGATCTG
AvaI 213 CCCGAG C^[CT]CG[GA]G
1236 CCCGAG
BamHI 9 GGATCC G^GATCC
98 GGATCC
BsaBI 99 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
825 GATCATCATC
BsgI 571 CTGCAC GTGCAG(16/14)
1115 GTGCAG
BsoBI 213 CCCGAG C^[CT]CG[GA]G
1236 CCCGAG
BstAPI 1058 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1072 GCAGACGATGC
EaeI 353 CGGCCA [CT]^GGCC[GA]
818 TGGCCA
EcoNI 153 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
315 CCTCTCCAAGG
MamI 99 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
825 GATCATCATC
NspI 524 GCATGC [GA]CATG^[CT]
1194 GCATGT
TaqI 41 TCGA T^CGA
383 TCGA
TfiI 165 GATTC G^A[AT]TC
640 GATTC
TthHB8I 41 TCGA T^CGA
383 TCGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanII BbvI BmyI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI XhoII