Restriction map for
pFN21AE5534
(Circular) (Six-base) MAPSORT of: pFN21AE5534.seq Check: 4848 from: 1 to: 6016
pFN21AE5534 6016 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2335 3158 2335
Size: 823 5193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3258 3359 1937
Size: 1321 101 4594
Fragments arranged by size:
4594 1321 101
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2624 2728 4079 5463 5731 1632
Size: 442 550 104 1351 1384 268 1917
Fragments arranged by size:
1917 1384 1351 550 442 268 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 2691 1166
Size: 1525 4491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2601 2755 1184
Size: 1417 154 4445
Fragments arranged by size:
4445 1417 154
AlwNI CAG_nnn'CTG
Cuts at: 2350 2488 3759 5331 2350
Size: 138 1271 1572 3035
Fragments arranged by size:
3035 1572 1271 138
ApaI G_GGCC'C
Cuts at: 2550 3174 3242 2550
Size: 624 68 5324
Fragments arranged by size:
5324 624 68
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2843 3325 3551 3703 5812 1493
Size: 1350 482 226 152 2109 1697
Fragments arranged by size:
2109 1697 1350 482 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 3241 3341 1870
Size: 73 275 1023 100 4545
Fragments arranged by size:
4545 1023 275 100 73
BamHI G'GATC_C
Cuts at: 2014 2103 3346 3713 2014
Size: 89 1243 367 4317
Fragments arranged by size:
4317 1243 367 89
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2550 2958 3174 3242 3335 1391
Size: 1159 408 216 68 93 4072
Fragments arranged by size:
4072 1159 408 216 93 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2612 928
Size: 971 713 4332
Fragments arranged by size:
4332 971 713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2285 2319 4207 4241 1903
Size: 34 348 34 1888 34 3678
Fragments arranged by size:
3678 1888 348 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2084 3908 5118 2084
Size: 1824 1210 2982
Fragments arranged by size:
2982 1824 1210
BclI T'GATC_A
Cuts at: 1548 2026 1548
Size: 478 5538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 3309 3433 3309
Size: 124 5892
Bme1580I G_kGCm'C
Cuts at: 1790 2550 3174 3242 4077 5233 1790
Size: 760 624 68 835 1156 2573
Fragments arranged by size:
2573 1156 835 760 624 68
BmgBI CAC'GTC
Cuts at: 1318 2689 1318
Size: 1371 4645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 2807 3747
Size: 416 53 83 399 24 1311 79 940
Cuts at: 3747 4705 442
Size: 958 1753
Fragments arranged by size:
1753 1311 958 940 416 399 83 79
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2946 4676 1239
Size: 1707 1730 2579
Fragments arranged by size:
2579 1730 1707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2524 4141 5006 5304 2524
Size: 1617 865 298 3236
Fragments arranged by size:
3236 1617 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2575 3274 4679 882
Size: 617 579 497 699 1405 2219
Fragments arranged by size:
2219 1405 699 617 579 497
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 2108 2834 6010 2108
Size: 726 3176 2114
Fragments arranged by size:
3176 2114 726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3041 3823 4205 4840
Size: 53 83 186 936 1508 782 382 635
Cuts at: 4840 275
Size: 1451
Fragments arranged by size:
1508 1451 936 782 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2645 2966 1186
Size: 1099 360 321 4236
Fragments arranged by size:
4236 1099 360 321
BseYI C'CCAG_C
Cuts at: 3219 5219 5715 3219
Size: 2000 496 3520
Fragments arranged by size:
3520 2000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 3141 1163
Size: 573 825 580 4038
Fragments arranged by size:
4038 825 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2958 3248 4077 4162 5233 2277
Size: 681 290 829 85 1071 3060
Fragments arranged by size:
3060 1071 829 681 290 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2550 2958 3174 3242 3248 3335
Size: 399 487 273 408 216 68 6 87
Cuts at: 3335 4077 4162 5233 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 408 399 273 216
87 85 68 6
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 2883 3905 5635 2883
Size: 1022 1730 3264
Fragments arranged by size:
3264 1730 1022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 2650 3158 3371 844
Size: 1491 315 508 213 3489
Fragments arranged by size:
3489 1491 508 315 213
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3079 4511 4685 65
Size: 1183 1831 1432 174 1396
Fragments arranged by size:
1831 1432 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3069 3083 794
Size: 2275 14 3727
Fragments arranged by size:
3727 2275 14
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2014 2103 2485 3288 3346 3713 4098 4115
Size: 42 89 382 803 58 367 385 17
Cuts at: 4115 5567 5653 5665 6011 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 803 385 382 367 346 89
86 58 42 17 12
BstZ17I GTA'TAC
Cuts at: 3259 3259
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 2079 513
Size: 552 1014 4450
Fragments arranged by size:
4450 1014 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2860 487
Size: 683 56 1634 3643
Fragments arranged by size:
3643 1634 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 2339 2737 3532 4326 4346 2313
Size: 26 398 795 794 20 3983
Fragments arranged by size:
3983 795 794 398 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DraIII CAC_nnn'GTG
Cuts at: 3069 3069
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2823 3463
Size: 54 1425 50 275 18 528 465 640
Cuts at: 3463 4352 5981 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 640 528 465 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2591 3946 1353
Size: 1238 1355 3423
Fragments arranged by size:
3423 1355 1238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 4603 4977 5123 387
Size: 1064 138 717 2297 374 146 1280
Fragments arranged by size:
2297 1280 1064 717 374 146 138
EcoICRI GAG'CTC
Cuts at: 2956 2956
Size: 6016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2624 2728 2946 4079 4676 5463
Size: 393 442 550 104 218 1133 597 787
Cuts at: 5463 5731 1239
Size: 268 1524
Fragments arranged by size:
1524 1133 787 597 550 442 393 268
218 104
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2324 2162
Size: 162 5854
EcoO109I rG'GnC_Cy
Cuts at: 2300 2547 2722 3171 3238 2300
Size: 247 175 449 67 5078
Fragments arranged by size:
5078 449 247 175 67
EcoRI G'AATT_C
Cuts at: 2843 3325 3703 2843
Size: 482 378 5156
Fragments arranged by size:
5156 482 378
FspI TGC'GCA
Cuts at: 2063 4522 2063
Size: 2459 3557
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2688 2720 4657 4689 4731
Size: 32 135 32 1116 32 1937 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 1937 1116 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5130 5314 1305
Size: 3825 184 2007
Fragments arranged by size:
3825 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2825 10
Size: 54 1425 343 993 3201
Fragments arranged by size:
3201 1425 993 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2165 2691 2757 3974 4333 4492
Size: 648 981 18 526 66 1217 359 159
Cuts at: 4492 518
Size: 2042
Fragments arranged by size:
2042 1217 981 648 526 359 159 66
18
MspA1I CmG'CkG
Cuts at: 1606 2230 3432 4109 5257 5502 1606
Size: 624 1202 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 677 624 245
NcoI C'CATG_G
Cuts at: 513 1065 2079 513
Size: 552 1014 4450
Fragments arranged by size:
4450 1014 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2533 3203 3374 4919 1132
Size: 97 1304 670 171 1545 2229
Fragments arranged by size:
2229 1545 1304 670 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 4636
PfoI T'CCnGG_A
Cuts at: 2706 3129 2706
Size: 423 5593
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2360 2392 4085 4117 5598 5630 2360
Size: 32 1693 32 1481 32 2746
Fragments arranged by size:
2746 1693 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 2722
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2546 3170 3238 2546
Size: 624 68 5324
Fragments arranged by size:
5324 624 68
PstI C_TGCA'G
Cuts at: 830 2308 2346 2895 3039 3368 830
Size: 1478 38 549 144 329 3478
Fragments arranged by size:
3478 1478 549 329 144 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2230 2230
Size: 6016
SacI G_AGCT'C
Cuts at: 2958 2958
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2591 2591
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2304 2342 2422 2437 2891 3035 3364
Size: 219 1259 38 80 15 454 144 329
Cuts at: 3364 4499 5180 5371 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1259 1135 681 454 329 219 191
144 80 38 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2539 4120 5021 5283 820
Size: 197 926 596 1581 901 262 1553
Fragments arranged by size:
1581 1553 926 901 596 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 2533 3374 1132
Size: 1401 841 3774
Fragments arranged by size:
3774 1401 841
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2636 1517
Size: 1119 4897
StyI C'CwwG_G
Cuts at: 513 1065 2079 2256 2325 2654 3264 3455 513
Size: 552 1014 177 69 329 610 191 3074
Fragments arranged by size:
3074 1014 610 552 329 191 177 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2346 2612 4043 4060 4213 4398 4843
Size: 1255 766 266 1431 17 153 185 445
Cuts at: 4843 325
Size: 1498
Fragments arranged by size:
1498 1431 1255 766 445 266 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4262 5974 96
Size: 274 80 33 51 1443 2285 1712 138
Fragments arranged by size:
2285 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2527 2559 5976 6008 2527
Size: 32 3417 32 2535
Fragments arranged by size:
3417 2535 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3161 3161
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2372 4145 2372
Size: 1773 4243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI Bsu36I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI