(Linear) (Six-base) MAPSORT of: KIEE5534.seq Check: 1994 from: 1 to: 1311 KIEE5534.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 330 1153 1311 Size: 330 823 158 Fragments arranged by size: 823 330 158 AccI GT'mk_AC Cuts at: 0 1253 1311 Size: 1253 58 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 619 723 1311 Size: 69 550 104 588 Fragments arranged by size: 588 550 104 69 AleI CACnn'nnGTG Cuts at: 0 686 1311 Size: 686 625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 596 750 1311 Size: 596 154 561 Fragments arranged by size: 596 561 154 AlwNI CAG_nnn'CTG Cuts at: 0 345 483 1311 Size: 345 138 828 Fragments arranged by size: 828 345 138 ApaI G_GGCC'C Cuts at: 0 545 1169 1237 1311 Size: 545 624 68 74 Fragments arranged by size: 624 545 74 68 ApoI r'AATT_y Cuts at: 0 838 1311 Size: 838 473 AvaI C'yCGr_G Cuts at: 0 213 1236 1311 Size: 213 1023 75 Fragments arranged by size: 1023 213 75 BamHI G'GATC_C Cuts at: 0 9 98 1311 Size: 9 89 1213 Fragments arranged by size: 1213 89 9 BanII G_rGCy'C Cuts at: 0 545 953 1169 1237 1311 Size: 545 408 216 68 74 Fragments arranged by size: 545 408 216 74 68 BbsI GAAGACnn'nnnn_ Cuts at: 0 607 1311 Size: 607 704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 280 314 1311 Size: 280 34 997 Fragments arranged by size: 997 280 34 BciVI GTATCCnnnnn_n' Cuts at: 0 79 1311 Size: 79 1232 BclI T'GATC_A Cuts at: 0 21 1311 Size: 21 1290 BlpI GC'TnA_GC Cuts at: 0 1304 1311 Size: 1304 7 Bme1580I G_kGCm'C Cuts at: 0 545 1169 1237 1311 Size: 545 624 68 74 Fragments arranged by size: 624 545 74 68 BmgBI CAC'GTC Cuts at: 0 684 1311 Size: 684 627 BmrI ACTGGGnnnn_n' Cuts at: 0 723 802 1311 Size: 723 79 509 Fragments arranged by size: 723 509 79 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 648 1311 Size: 648 663 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 941 1311 Size: 941 370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 519 1311 Size: 519 792 BsaI GGTCTCn'nnnn_ Cuts at: 0 73 570 1269 1311 Size: 73 497 699 42 Fragments arranged by size: 699 497 73 42 BsaBI GATnn'nnATC Cuts at: 0 103 829 1311 Size: 103 726 482 Fragments arranged by size: 726 482 103 BsaHI Gr'CG_yC Cuts at: 0 1036 1311 Size: 1036 275 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 640 961 1311 Size: 280 360 321 350 Fragments arranged by size: 360 350 321 280 BseYI C'CCAG_C Cuts at: 0 1214 1311 Size: 1214 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1136 1311 Size: 556 580 175 Fragments arranged by size: 580 556 175 BsiHKAI G_wGCw'C Cuts at: 0 272 953 1243 1311 Size: 272 681 290 68 Fragments arranged by size: 681 290 272 68 Bsp1286I G_dGCh'C Cuts at: 0 272 545 953 1169 1237 1243 1311 Size: 272 273 408 216 68 6 68 Fragments arranged by size: 408 273 272 216 68 68 6 BspHI T'CATG_A Cuts at: 0 878 1311 Size: 878 433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 330 645 1153 1311 Size: 330 315 508 158 Fragments arranged by size: 508 330 315 158 BsrDI GCAATG_nn' Cuts at: 0 1074 1311 Size: 1074 237 BstAPI GCAn_nnn'nTGC Cuts at: 0 1064 1078 1311 Size: 1064 14 233 Fragments arranged by size: 1064 233 14 BstYI r'GATC_y Cuts at: 0 9 98 480 1283 1311 Size: 9 89 382 803 28 Fragments arranged by size: 803 382 89 28 9 BstZ17I GTA'TAC Cuts at: 0 1254 1311 Size: 1254 57 BtgI C'CryG_G Cuts at: 0 74 1311 Size: 74 1237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 855 1311 Size: 855 456 BtsI GCAGTG_nn' Cuts at: 0 308 334 732 1311 Size: 308 26 398 579 Fragments arranged by size: 579 398 308 26 DraIII CAC_nnn'GTG Cuts at: 0 1064 1311 Size: 1064 247 EaeI y'GGCC_r Cuts at: 0 353 818 1311 Size: 353 465 493 Fragments arranged by size: 493 465 353 EarI CTCTTCn'nnn_ Cuts at: 0 586 1311 Size: 586 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 301 1311 Size: 301 1010 EcoICRI GAG'CTC Cuts at: 0 951 1311 Size: 951 360 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 619 723 941 1311 Size: 69 550 104 218 370 Fragments arranged by size: 550 370 218 104 69 EcoNI CCTnn'n_nnAGG Cuts at: 0 157 319 1311 Size: 157 162 992 Fragments arranged by size: 992 162 157 EcoO109I rG'GnC_Cy Cuts at: 0 295 542 717 1166 1233 1311 Size: 295 247 175 449 67 78 Fragments arranged by size: 449 295 247 175 78 67 EcoRI G'AATT_C Cuts at: 0 838 1311 Size: 838 473 FspI TGC'GCA Cuts at: 0 58 1311 Size: 58 1253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 683 715 1311 Size: 683 32 596 Fragments arranged by size: 683 596 32 MscI TGG'CCA Cuts at: 0 820 1311 Size: 820 491 MslI CAynn'nnrTG Cuts at: 0 142 160 686 752 1311 Size: 142 18 526 66 559 Fragments arranged by size: 559 526 142 66 18 MspA1I CmG'CkG Cuts at: 0 225 1311 Size: 225 1086 NcoI C'CATG_G Cuts at: 0 74 1311 Size: 74 1237 NspI r_CATG'y Cuts at: 0 528 1198 1311 Size: 528 670 113 Fragments arranged by size: 670 528 113 PflMI CCAn_nnn'nTGG Cuts at: 0 483 1311 Size: 483 828 PfoI T'CCnGG_A Cuts at: 0 701 1124 1311 Size: 701 423 187 Fragments arranged by size: 701 423 187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 355 387 1311 Size: 355 32 924 Fragments arranged by size: 924 355 32 PpuMI rG'GwC_Cy Cuts at: 0 717 1311 Size: 717 594 PspOMI G'GGCC_C Cuts at: 0 541 1165 1233 1311 Size: 541 624 68 78 Fragments arranged by size: 624 541 78 68 PstI C_TGCA'G Cuts at: 0 303 341 890 1034 1311 Size: 303 38 549 144 277 Fragments arranged by size: 549 303 277 144 38 PvuII CAG'CTG Cuts at: 0 225 1311 Size: 225 1086 SacI G_AGCT'C Cuts at: 0 953 1311 Size: 953 358 SapI GCTCTTCn'nnn_ Cuts at: 0 586 1311 Size: 586 725 SfcI C'TryA_G Cuts at: 0 299 337 417 432 886 1030 1311 Size: 299 38 80 15 454 144 281 Fragments arranged by size: 454 299 281 144 80 38 15 SmlI C'TyrA_G Cuts at: 0 534 1311 Size: 534 777 SphI G_CATG'C Cuts at: 0 528 1311 Size: 528 783 StuI AGG'CCT Cuts at: 0 631 1311 Size: 631 680 StyI C'CwwG_G Cuts at: 0 74 251 320 649 1259 1311 Size: 74 177 69 329 610 52 Fragments arranged by size: 610 329 177 74 69 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 341 607 1311 Size: 341 266 704 Fragments arranged by size: 704 341 266 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 522 554 1311 Size: 522 32 757 Fragments arranged by size: 757 522 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1156 1311 Size: 1156 155 XmnI GAAnn'nnTTC Cuts at: 0 367 1311 Size: 367 944 Enzymes that do cut: AarI AccI AcuI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanII BbsI BcgI BciVI BclI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstAPI BstYI BstZ17I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I MscI MslI MspA1I NcoI NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI SfcI SmlI SphI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BfrBI BglI BglII BmtI Bpu10I BsaAI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I ClaI CspCI DraI DrdI EagI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI