(Circular) (Six-base) MAPSORT of: pFN21AE5788.seq Check: 7256 from: 1 to: 7045 pFN21AE5788 7045 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2015 3388 4855 278 Size: 53 83 186 936 479 1373 1467 2468 Fragments arranged by size: 2468 1467 1373 936 479 186 83 53 AccI GT'mk_AC Cuts at: 1937 4388 1937 Size: 2451 4594 Acc65I G'GTAC_C Cuts at: 3992 4366 3992 Size: 374 6671 AclI AA'CG_TT Cuts at: 1563 5172 5545 1563 Size: 3609 373 3063 Fragments arranged by size: 3609 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 3306 3534 5108 6492 6760 1632 Size: 395 1279 228 1574 1384 268 1917 Fragments arranged by size: 1917 1574 1384 1279 395 268 228 AflII C'TTAA_G Cuts at: 820 1017 3326 3860 820 Size: 197 2309 534 4005 Fragments arranged by size: 4005 2309 534 197 AflIII A'CryG_T Cuts at: 1203 3243 4236 5944 6921 1203 Size: 2040 993 1708 977 1327 Fragments arranged by size: 2040 1708 1327 993 977 AgeI A'CCGG_T Cuts at: 5876 5876 Size: 7045 AhdI GACnn_n'nnGTC Cuts at: 3384 5774 3384 Size: 2390 4655 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7045 AlwNI CAG_nnn'CTG Cuts at: 2993 4788 6360 2993 Size: 1795 1572 3678 Fragments arranged by size: 3678 1795 1572 ApaLI G'TGCA_C Cuts at: 5102 6258 5102 Size: 1156 5889 ApoI r'AATT_y Cuts at: 1493 2184 2592 3591 3683 4224 4354 4580 4732 Size: 691 408 999 92 541 130 226 152 Cuts at: 4732 6841 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 999 691 541 408 226 152 130 92 AseI AT'TA_AT Cuts at: 160 1032 5599 160 Size: 872 4567 1606 Fragments arranged by size: 4567 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 AvaI C'yCGr_G Cuts at: 1870 1943 4370 1870 Size: 73 2427 4545 Fragments arranged by size: 4545 2427 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3314 3347 3314 Size: 33 7012 BamHI G'GATC_C Cuts at: 4375 4742 4375 Size: 367 6678 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3605 3992 4366 5821 618 Size: 325 217 627 1818 387 374 1455 1842 Fragments arranged by size: 1842 1818 1455 627 387 374 325 217 BanII G_rGCy'C Cuts at: 1391 4364 1391 Size: 2973 4072 BbeI G_GCGC'C Cuts at: 3609 3609 Size: 7045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2403 928 Size: 971 159 345 5570 Fragments arranged by size: 5570 971 345 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5236 5270 1903 Size: 34 3299 34 3678 Fragments arranged by size: 3678 3299 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4937 6147 4937 Size: 1210 5835 BclI T'GATC_A Cuts at: 1548 2856 1548 Size: 1308 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5656 136 Size: 107 122 71 631 605 3984 1525 Fragments arranged by size: 3984 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7040 7040 Size: 7045 BlpI GC'TnA_GC Cuts at: 4200 4462 4200 Size: 262 6783 Bme1580I G_kGCm'C Cuts at: 1790 5106 6262 1790 Size: 3316 1156 2573 Fragments arranged by size: 3316 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4776 5734 442 Size: 416 53 83 399 24 3359 958 1753 Fragments arranged by size: 3359 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3677 5705 1239 Size: 2438 2028 2579 Fragments arranged by size: 2579 2438 2028 Bpu10I CC'TnA_GC Cuts at: 3219 3219 Size: 7045 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 3945 5170 6035 6333 2234 Size: 1711 1225 865 298 2946 Fragments arranged by size: 2946 1711 1225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5708 882 Size: 617 4209 2219 Fragments arranged by size: 4209 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4836 493 Size: 711 3632 2702 Fragments arranged by size: 3632 2702 711 BsaBI GATnn'nnATC Cuts at: 7039 7039 Size: 7045 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3385 3606 4852 Size: 53 83 186 936 479 1373 221 1246 Cuts at: 4852 5234 5869 275 Size: 382 635 1451 Fragments arranged by size: 1451 1373 1246 936 635 479 382 221 186 83 53 BsaWI w'CCGG_w Cuts at: 3670 5478 5876 6150 6297 6708 3670 Size: 1808 398 274 147 411 4007 Fragments arranged by size: 4007 1808 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6820 Fragments arranged by size: 6820 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7045 BseYI C'CCAG_C Cuts at: 2176 6248 6744 2176 Size: 4072 496 2477 Fragments arranged by size: 4072 2477 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6472 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4495 5256 6284 1540 Size: 275 186 2494 761 1028 2301 Fragments arranged by size: 2494 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2740 3318 4293 5106 5191 6262 2740 Size: 578 975 813 85 1071 3523 Fragments arranged by size: 3523 1071 975 813 578 85 BsmI GAATG_Cn' Cuts at: 2798 3604 4556 4649 2798 Size: 806 952 93 5194 Fragments arranged by size: 5194 952 806 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2740 3318 4293 4364 5106 5191 6262 Size: 399 950 578 975 71 742 85 1071 Cuts at: 6262 1391 Size: 2174 Fragments arranged by size: 2174 1071 975 950 742 578 399 85 71 BspEI T'CCGG_A Cuts at: 6708 6708 Size: 7045 BspHI T'CATG_A Cuts at: 4934 6664 4934 Size: 1730 5315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4400 844 Size: 3556 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4932 1394 Size: 429 3109 3507 Fragments arranged by size: 3507 3109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 5540 5714 65 Size: 1183 1239 3053 174 1396 Fragments arranged by size: 3053 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5689 5876 1700 Size: 110 3879 187 2869 Fragments arranged by size: 3879 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2683 3334 96 Size: 2587 651 3807 Fragments arranged by size: 3807 2587 651 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7045 BssSI C'ACGA_G Cuts at: 2238 5105 6117 2238 Size: 2867 1012 3166 Fragments arranged by size: 3166 2867 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7045 BstBI TT'CG_AA Cuts at: 2410 5849 2410 Size: 3439 3606 BstEII G'GTnAC_C Cuts at: 1184 3787 1184 Size: 2603 4442 BstYI r'GATC_y Cuts at: 1972 4375 4742 5127 5144 6596 6682 6694 7040 Size: 2403 367 385 17 1452 86 12 346 Cuts at: 7040 1972 Size: 1977 Fragments arranged by size: 2403 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2577 2577 Size: 7045 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6306 Fragments arranged by size: 6306 683 56 BtsI GCAGTG_nn' Cuts at: 2126 4561 5355 5375 2126 Size: 2435 794 20 3796 Fragments arranged by size: 3796 2435 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7045 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4259 4294 529 Size: 35 3695 35 3280 Fragments arranged by size: 3695 3280 35 35 DraI TTT'AAA Cuts at: 2199 2824 3154 4349 2199 Size: 625 330 1195 4895 Fragments arranged by size: 4895 1195 625 330 DraIII CAC_nnn'GTG Cuts at: 2315 2315 Size: 7045 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3907 6052 809 Size: 507 2591 2145 1802 Fragments arranged by size: 2591 2145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4492 5381 7010 Size: 54 1425 50 275 18 2662 889 1629 Cuts at: 7010 8 Size: 43 Fragments arranged by size: 2662 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4492 1537 Size: 275 2680 4090 Fragments arranged by size: 4090 2680 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 3282 4004 4104 4300 4975 1353 Size: 693 1236 722 100 196 675 3423 Fragments arranged by size: 3423 1236 722 693 675 196 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5632 6006 6152 387 Size: 1064 138 4043 374 146 1280 Fragments arranged by size: 4043 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 3306 3534 3677 5108 5705 6492 Size: 393 395 1279 228 143 1431 597 787 Cuts at: 6492 6760 1239 Size: 268 1524 Fragments arranged by size: 1524 1431 1279 787 597 395 393 268 228 143 EcoRI G'AATT_C Cuts at: 3683 4354 4732 3683 Size: 671 378 5996 Fragments arranged by size: 5996 671 378 EcoRV GAT'ATC Cuts at: 4321 4321 Size: 7045 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2057 2228 2638 3320 2057 Size: 171 410 682 5782 Fragments arranged by size: 5782 682 410 171 FspI TGC'GCA Cuts at: 5551 5551 Size: 7045 HaeII r_GCGC'y Cuts at: 1678 3609 6192 1678 Size: 1931 2583 2531 Fragments arranged by size: 2583 2531 1931 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 3370 3402 3828 Size: 32 135 32 430 32 1336 32 426 Cuts at: 3828 3860 5686 5718 5760 5792 1373 Size: 32 1826 32 42 32 2626 Fragments arranged by size: 2626 1826 1336 430 426 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2342 2560 3967 4389 4635 669 Size: 988 281 404 218 1407 422 246 3079 Fragments arranged by size: 3079 1407 988 422 404 281 246 218 HindIII A'AGCT_T Cuts at: 748 2087 2414 2514 3075 3302 3479 3528 3863 Size: 1339 327 100 561 227 177 49 335 Cuts at: 3863 748 Size: 3930 Fragments arranged by size: 3930 1339 561 335 327 227 177 100 49 HpaI GTT'AAC Cuts at: 2560 4635 2560 Size: 2075 4970 KasI G'GCGC_C Cuts at: 3605 3605 Size: 7045 KpnI G_GTAC'C Cuts at: 3996 4370 3996 Size: 374 6671 MfeI C'AATT_G Cuts at: 4644 4644 Size: 7045 MluI A'CGCG_T Cuts at: 6921 6921 Size: 7045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6159 6343 1305 Size: 4854 184 2007 Fragments arranged by size: 4854 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5223 Fragments arranged by size: 5223 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 4337 5003 5362 5521 518 Size: 648 1090 2081 666 359 159 2042 Fragments arranged by size: 2081 2042 1090 666 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2530 2990 3464 3572 4204 4461 5138 6286 Size: 924 460 474 108 632 257 677 1148 Cuts at: 6286 6531 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 924 677 632 474 460 257 245 108 NarI GG'CG_CC Cuts at: 3606 3606 Size: 7045 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7045 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7045 NotI GC'GGCC_GC Cuts at: 4492 4492 Size: 7045 NspI r_CATG'y Cuts at: 1132 1229 2150 2498 3247 4240 4403 5948 1132 Size: 97 921 348 749 993 163 1545 2229 Fragments arranged by size: 2229 1545 993 921 749 348 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7045 PciI A'CATG_T Cuts at: 3243 4236 5944 3243 Size: 993 1708 4344 Fragments arranged by size: 4344 1708 993 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7045 PmeI GTTT'AAAC Cuts at: 4349 4349 Size: 7045 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5114 5146 6627 6659 5114 Size: 32 1481 32 5500 Fragments arranged by size: 5500 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7045 PsiI TTA'TAA Cuts at: 3749 4615 3749 Size: 866 6179 PstI C_TGCA'G Cuts at: 830 4209 4397 830 Size: 3379 188 3478 Fragments arranged by size: 3478 3379 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7045 PvuII CAG'CTG Cuts at: 2530 2990 3572 4204 2530 Size: 460 582 632 5371 Fragments arranged by size: 5371 632 582 460 SalI G'TCGA_C Cuts at: 1936 4387 1936 Size: 2451 4594 SbfI CC_TGCA'GG Cuts at: 4397 4397 Size: 7045 ScaI AGT'ACT Cuts at: 2244 5293 7005 2244 Size: 3049 1712 2284 Fragments arranged by size: 3049 2284 1712 SfcI C'TryA_G Cuts at: 826 1045 4089 4205 4393 5528 6209 6400 826 Size: 219 3044 116 188 1135 681 191 1471 Fragments arranged by size: 3044 1471 1135 681 219 191 188 116 SfoI GGC'GCC Cuts at: 3607 3607 Size: 7045 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7045 SmaI CCC'GGG Cuts at: 1872 4372 1872 Size: 2500 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 3326 3860 3960 5149 6050 Size: 197 926 270 1113 534 100 1189 901 Cuts at: 6050 6312 820 Size: 262 1553 Fragments arranged by size: 1553 1189 1113 926 901 534 270 262 197 100 SnaBI TAC'GTA Cuts at: 493 4836 493 Size: 4343 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7045 SphI G_CATG'C Cuts at: 1132 2150 4403 1132 Size: 1018 2253 3774 Fragments arranged by size: 3774 2253 1018 SspI AAT'ATT Cuts at: 5 52 4969 5 Size: 47 4917 2081 Fragments arranged by size: 4917 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7045 StyI C'CwwG_G Cuts at: 513 1065 2004 2848 4484 513 Size: 552 939 844 1636 3074 Fragments arranged by size: 3074 1636 939 844 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3988 5072 5089 5242 5427 5872 325 Size: 1255 2408 1084 17 153 185 445 1498 Fragments arranged by size: 2408 1498 1255 1084 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2242 2683 3334 Size: 274 80 33 51 1443 265 441 651 Cuts at: 3334 5291 7003 96 Size: 1957 1712 138 Fragments arranged by size: 1957 1712 1443 651 441 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7005 7037 7005 Size: 32 7013 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7045 XbaI T'CTAG_A Cuts at: 4381 4381 Size: 7045 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7045 XmaI C'CCGG_G Cuts at: 1870 4370 1870 Size: 2500 4545 XmnI GAAnn'nnTTC Cuts at: 2208 3078 5174 2208 Size: 870 2096 4079 Fragments arranged by size: 4079 2096 870 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2013 3386 4853 276 Size: 53 83 186 936 479 1373 1467 2468 Fragments arranged by size: 2468 1467 1373 936 479 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI