Restriction map for
pFN21AE0929
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 439 CGCG CG^CG
AcsI 229 GAATTC [GA]^AATT[CT]
AfaI 990 GTAC GT^AC
AflIII 180 ACGTGT A^C[GA][CT]GT
Alw44I 442 GTGCAC G^TGCAC
AocI 937 CCTGAGG CC^T[ACGT]AGG
Aor51HI 623 AGCGCT AGC^GCT
ApaLI 442 GTGCAC G^TGCAC
ApoI 229 GAATTC [GA]^AATT[CT]
AspHI 442 GTGCAC G[AT]GC[AT]^C
AvaI 1002 CCCGAG C^[CT]CG[GA]G
AvrII 1011 CCTAGG C^CTAGG
BamHI 1016 GGATCC G^GATCC
BbsI 595 GTCTTC GAAGAC(2/6)
BfaI 1012 CTAG C^TAG
BglI 153 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1011 CCTAGG C^CTAGG
BpuAI 595 GTCTTC GAAGAC(2/6)
BsaBI 1066 GATAGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 442 GTGCAC G[AT]GC[AT]^C
BsoBI 1002 CCCGAG C^[CT]CG[GA]G
BspMI 527 ACCTGC ACCTGC(4/8)
BstEII 95 GGTCACC G^GT[ACGT]ACC
BstPI 95 GGTCACC G^GT[ACGT]ACC
BstUI 439 CGCG CG^CG
Bsu36I 937 CCTGAGG CC^T[ACGT]AGG
DsaI 784 CCACGG C^C[GA][CT]GG
Eco47III 623 AGCGCT AGC^GCT
Eco81I 937 CCTGAGG CC^T[ACGT]AGG
EcoO65I 95 GGTCACC G^GT[ACGT]ACC
EcoRI 229 GAATTC G^AATTC
FauI 409 GCGGG CCCGC(4/6)
HaeII 623 AGCGCT [GA]GCGC^[CT]
HindIII 878 AAGCTT A^AGCTT
MaeI 1012 CTAG C^TAG
MamI 1066 GATAGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 439 CGCG CG^CG
PflMI 138 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 990 GTAC GT^AC
SfaNI 483 GATGC GCATC(5/9)
SfcI 963 CTACAG C^T[GA][CT]AG
SmlI 511 CTCAAG C^T[CT][GA]AG
Tsp509I 230 AATT ^AATT
Van91I 138 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 521 CCAACAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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ApaI 134 GGGCCC GGGCC^C
165 GGGCCC
BalI 543 TGGCCA TGG^CCA
1140 TGGCCA
BanI 472 GGTGCC G^G[CT][GA]CC
847 GGTGCC
BbrPI 179 CACGTG CAC^GTG
589 CACGTG
BpmI 205 CTGGAG CTGGAG(16/14)
995 CTGGAG
BsaI 46 GAGACC GGTCTC(1/5)
664 GAGACC
BsmAI 46 GAGAC GTCTC(1/5)
664 GAGAC
BstYI 302 AGATCC [GA]^GATC[CT]
1016 GGATCC
CfoI 486 GCGC GCG^C
624 GCGC
HhaI 486 GCGC GCG^C
624 GCGC
HinP1I 486 GCGC G^CGC
624 GCGC
MflI 302 AGATCC [GA]^GATC[CT]
1016 GGATCC
MluNI 543 TGGCCA TGG^CCA
1140 TGGCCA
MscI 543 TGGCCA TGG^CCA
1140 TGGCCA
NlaIII 312 CATG CATG^
342 CATG
PmaCI 179 CACGTG CAC^GTG
589 CACGTG
PmlI 179 CACGTG CAC^GTG
589 CACGTG
PpuMI 941 AGGTCCT [GA]G^G[AT]CC[CT]
1024 AGGACCC
StuI 967 AGGCCT AGG^CCT
1080 AGGCCT
XhoII 302 AGATCC [GA]^GATC[CT]
1016 GGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BcnI BmyI BsaAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI StyI
TseI Tsp45I TspRI