(Circular) (Six-base) MAPSORT of: pFN21AE0929.seq Check: 4144 from: 1 to: 5860 pFN21AE0929 5860 bp With 182 enzymes: SgfI * March 28, 2012 13:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 2871 3923 5307 5575 1632 Size: 1235 4 1052 1384 268 1917 Fragments arranged by size: 1917 1384 1235 1052 268 4 AfeI AGC'GCT Cuts at: 2630 2630 Size: 5860 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2185 4759 5736 1203 Size: 982 2574 977 1327 Fragments arranged by size: 2574 1327 982 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 2684 3128 1166 Size: 1518 444 3898 Fragments arranged by size: 3898 1518 444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2636 2668 2636 Size: 32 5828 AlwNI CAG_nnn'CTG Cuts at: 2838 2932 2992 3603 5175 2838 Size: 94 60 611 1572 3523 Fragments arranged by size: 3523 1572 611 94 60 ApaI G_GGCC'C Cuts at: 2143 2174 2143 Size: 31 5829 ApaLI G'TGCA_C Cuts at: 2447 3917 5073 2447 Size: 1470 1156 3234 Fragments arranged by size: 3234 1470 1156 ApoI r'AATT_y Cuts at: 1493 2234 3169 3395 3547 5656 1493 Size: 741 935 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 741 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 3007 3185 1870 Size: 73 1064 178 4545 Fragments arranged by size: 4545 1064 178 73 AvrII C'CTAG_G Cuts at: 3016 3016 Size: 5860 BamHI G'GATC_C Cuts at: 3021 3190 3557 3021 Size: 169 367 5324 Fragments arranged by size: 5324 367 169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 2852 3181 4636 618 Size: 325 217 627 690 375 329 1455 1842 Fragments arranged by size: 1842 1455 690 627 375 329 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2143 2160 2174 3007 3114 3179 1391 Size: 700 52 17 14 833 107 65 4072 Fragments arranged by size: 4072 833 700 107 65 52 17 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2593 928 Size: 971 694 4195 Fragments arranged by size: 4195 971 694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2164 4471 136 Size: 107 122 71 631 605 492 2307 1525 Fragments arranged by size: 2307 1525 631 605 492 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2143 2174 2252 2451 2482 3921 5077 1790 Size: 353 31 78 199 31 1439 1156 2573 Fragments arranged by size: 2573 1439 1156 353 199 78 31 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 2172 2476 Size: 416 53 83 399 24 728 27 304 Cuts at: 2476 2668 3591 4549 442 Size: 192 923 958 1753 Fragments arranged by size: 1753 958 923 728 416 399 304 192 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 3021 4520 1239 Size: 992 790 1499 2579 Fragments arranged by size: 2579 1499 992 790 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 3985 4850 5148 2501 Size: 1484 865 298 3213 Fragments arranged by size: 3213 1484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 2663 4523 882 Size: 617 546 618 1860 2219 Fragments arranged by size: 2219 1860 618 617 546 BsaAI yAC'GTr Cuts at: 493 1204 2186 2596 2982 3651 493 Size: 711 982 410 386 669 2702 Fragments arranged by size: 2702 982 711 669 410 386 BsaBI GATnn'nnATC Cuts at: 3075 5854 3075 Size: 2779 3081 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 1178 Size: 30 165 30 1353 30 4252 Fragments arranged by size: 4252 1353 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 2082 2095 2245 2428 2715 2809 3097 3101 3106 Size: 13 150 183 287 94 288 4 5 Cuts at: 3106 5063 5559 2082 Size: 1957 496 2383 Fragments arranged by size: 2383 1957 496 288 287 183 150 94 13 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 3921 4006 5077 2451 Size: 1470 85 1071 3234 Fragments arranged by size: 3234 1470 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2143 2160 2174 2252 2451 2482 Size: 399 301 52 17 14 78 199 31 Cuts at: 2482 3007 3114 3179 3921 4006 5077 1391 Size: 525 107 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 525 399 301 199 107 85 78 65 52 31 17 14 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3215 844 Size: 1697 674 3489 Fragments arranged by size: 3489 1697 674 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 2100 1184 Size: 916 4944 BstYI r'GATC_y Cuts at: 1972 2307 3021 3190 3557 3942 3959 5411 5497 Size: 335 714 169 367 385 17 1452 86 Cuts at: 5497 5509 5855 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 714 385 367 346 335 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2943 2943 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2004 2789 513 Size: 552 939 785 3584 Fragments arranged by size: 3584 939 785 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2691 3376 4170 4190 2691 Size: 685 794 20 4361 Fragments arranged by size: 4361 794 685 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2328 2440 Size: 54 1425 50 275 18 321 177 112 Cuts at: 2440 2548 2961 3145 3307 4196 5825 8 Size: 108 413 184 162 889 1629 43 Fragments arranged by size: 1629 1425 889 413 321 275 184 177 162 112 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3790 1353 Size: 2437 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2867 2871 3021 3923 4520 5307 Size: 393 599 636 4 150 902 597 787 Cuts at: 5307 5575 1239 Size: 268 1524 Fragments arranged by size: 1524 902 787 636 599 597 393 268 150 4 EcoO109I rG'GnC_Cy Cuts at: 2117 2139 2170 2171 2947 3030 3140 2117 Size: 22 31 1 776 83 110 4837 Fragments arranged by size: 4837 776 110 83 31 22 1 EcoRI G'AATT_C Cuts at: 2234 3169 3547 2234 Size: 935 378 4547 Fragments arranged by size: 4547 935 378 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2632 5007 1678 Size: 954 2375 2531 Fragments arranged by size: 2531 2375 954 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2206 2238 2680 2712 2756 Size: 32 135 32 634 32 442 32 44 Cuts at: 2756 2788 4501 4533 4575 4607 1373 Size: 32 1713 32 42 32 2626 Fragments arranged by size: 2626 1713 634 442 135 44 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 2883 748 Size: 2135 3725 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 3147 10 Size: 54 1425 343 718 597 2723 Fragments arranged by size: 2723 1425 718 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2684 3128 3818 4177 4336 518 Size: 648 1089 429 444 690 359 159 2042 Fragments arranged by size: 2042 1089 690 648 444 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 2245 2719 2929 3101 3276 3953 5101 Size: 603 36 474 210 172 175 677 1148 Cuts at: 5101 5346 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 603 474 245 210 175 172 36 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4369 Fragments arranged by size: 4369 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 4819 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PmlI CAC'GTG Cuts at: 2186 2596 2186 Size: 410 5450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 2219 2660 2692 3929 3961 5442 5474 2187 Size: 32 441 32 1237 32 1481 32 2573 Fragments arranged by size: 2573 1481 1237 441 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 3030 2947 Size: 83 5777 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2139 2170 2139 Size: 31 5829 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2209 2245 2719 2929 3101 2209 Size: 36 474 210 172 4968 Fragments arranged by size: 4968 474 210 172 36 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2968 3208 4343 5024 5215 826 Size: 219 1923 240 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 240 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3964 4865 5127 820 Size: 197 926 573 1448 901 262 1553 Fragments arranged by size: 1553 1448 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 2974 3087 1517 Size: 1457 113 4290 Fragments arranged by size: 4290 1457 113 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2472 3016 3026 3299 513 Size: 552 939 264 204 544 10 273 3074 Fragments arranged by size: 3074 939 552 544 273 264 204 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 3887 3904 4057 4242 4687 325 Size: 1255 1149 1158 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1158 1149 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2994 4106 5818 Size: 274 80 33 51 1443 1017 1112 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1443 1112 1017 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 5820 5852 2931 Size: 32 2857 32 2939 Fragments arranged by size: 2939 2857 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 2533 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI