Restriction map for
pFN21AE0929
(Linear) (Six-base) MAPSORT of: KIEE0929.seq Check: 7423 from: 1 to: 1155
KIEE0929.orf
With 182 enzymes: SgfI *
March 22, 2012 15:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 866 1155
Size: 862 4 289
Fragments arranged by size:
862 289 4
AfeI AGC'GCT
Cuts at: 0 625 1155
Size: 625 530
AflIII A'CryG_T
Cuts at: 0 180 1155
Size: 180 975
AleI CACnn'nnGTG
Cuts at: 0 679 1123 1155
Size: 679 444 32
Fragments arranged by size:
679 444 32
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 631 663 1155
Size: 631 32 492
Fragments arranged by size:
631 492 32
AlwNI CAG_nnn'CTG
Cuts at: 0 833 927 987 1155
Size: 833 94 60 168
Fragments arranged by size:
833 168 94 60
ApaI G_GGCC'C
Cuts at: 0 138 169 1155
Size: 138 31 986
Fragments arranged by size:
986 138 31
ApaLI G'TGCA_C
Cuts at: 0 442 1155
Size: 442 713
ApoI r'AATT_y
Cuts at: 0 229 1155
Size: 229 926
AvaI C'yCGr_G
Cuts at: 0 1002 1155
Size: 1002 153
AvrII C'CTAG_G
Cuts at: 0 1011 1155
Size: 1011 144
BamHI G'GATC_C
Cuts at: 0 1016 1155
Size: 1016 139
BanI G'GyrC_C
Cuts at: 0 472 847 1155
Size: 472 375 308
Fragments arranged by size:
472 375 308
BanII G_rGCy'C
Cuts at: 0 86 138 155 169 1002 1109 1155
Size: 86 52 17 14 833 107 46
Fragments arranged by size:
833 107 86 52 46 17 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 1155
Size: 588 567
BglI GCCn_nnn'nGGC
Cuts at: 0 159 1155
Size: 159 996
Bme1580I G_kGCm'C
Cuts at: 0 138 169 247 446 477 1155
Size: 138 31 78 199 31 678
Fragments arranged by size:
678 199 138 78 31 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 167 471 663 1155
Size: 140 27 304 192 492
Fragments arranged by size:
492 304 192 140 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1016 1155
Size: 226 790 139
Fragments arranged by size:
790 226 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1155
Size: 496 659
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 658 1155
Size: 40 618 497
Fragments arranged by size:
618 497 40
BsaAI yAC'GTr
Cuts at: 0 181 591 977 1155
Size: 181 410 386 178
Fragments arranged by size:
410 386 181 178
BsaBI GATnn'nnATC
Cuts at: 0 1070 1155
Size: 1070 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 1155
Size: 751 30 374
Fragments arranged by size:
751 374 30
BseYI C'CCAG_C
Cuts at: 0 77 90 240 423 710 804 1092 1096
Size: 77 13 150 183 287 94 288 4
Cuts at: 1096 1101 1155
Size: 5 54
Fragments arranged by size:
288 287 183 150 94 77 54 13
5 4
BsiHKAI G_wGCw'C
Cuts at: 0 446 1155
Size: 446 709
Bsp1286I G_dGCh'C
Cuts at: 0 86 138 155 169 247 446 477 1002
Size: 86 52 17 14 78 199 31 525
Cuts at: 1002 1109 1155
Size: 107 46
Fragments arranged by size:
525 199 107 86 78 52 46 31
17 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1155
Size: 536 619
BstEII G'GTnAC_C
Cuts at: 0 95 1155
Size: 95 1060
BstYI r'GATC_y
Cuts at: 0 302 1016 1155
Size: 302 714 139
Fragments arranged by size:
714 302 139
Bsu36I CC'TnA_GG
Cuts at: 0 938 1155
Size: 938 217
BtgI C'CryG_G
Cuts at: 0 784 1155
Size: 784 371
BtsI GCAGTG_nn'
Cuts at: 0 686 1155
Size: 686 469
EaeI y'GGCC_r
Cuts at: 0 146 323 435 543 956 1140 1155
Size: 146 177 112 108 413 184 15
Fragments arranged by size:
413 184 177 146 112 108 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 862 866 1016 1155
Size: 226 636 4 150 139
Fragments arranged by size:
636 226 150 139 4
EcoO109I rG'GnC_Cy
Cuts at: 0 112 134 165 166 942 1025 1135 1155
Size: 112 22 31 1 776 83 110 20
Fragments arranged by size:
776 112 110 83 31 22 20 1
EcoRI G'AATT_C
Cuts at: 0 229 1155
Size: 229 926
HaeII r_GCGC'y
Cuts at: 0 627 1155
Size: 627 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 675 707 751 783 1155
Size: 201 32 442 32 44 32 372
Fragments arranged by size:
442 372 201 44 32 32 32
HindIII A'AGCT_T
Cuts at: 0 878 1155
Size: 878 277
MscI TGG'CCA
Cuts at: 0 545 1142 1155
Size: 545 597 13
Fragments arranged by size:
597 545 13
MslI CAynn'nnrTG
Cuts at: 0 250 679 1123 1155
Size: 250 429 444 32
Fragments arranged by size:
444 429 250 32
MspA1I CmG'CkG
Cuts at: 0 204 240 714 924 1096 1155
Size: 204 36 474 210 172 59
Fragments arranged by size:
474 210 204 172 59 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1155
Size: 144 1011
PmlI CAC'GTG
Cuts at: 0 181 591 1155
Size: 181 410 564
Fragments arranged by size:
564 410 181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 655 687 1155
Size: 182 32 441 32 468
Fragments arranged by size:
468 441 182 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 942 1025 1155
Size: 942 83 130
Fragments arranged by size:
942 130 83
PspOMI G'GGCC_C
Cuts at: 0 134 165 1155
Size: 134 31 990
Fragments arranged by size:
990 134 31
PvuII CAG'CTG
Cuts at: 0 204 240 714 924 1096 1155
Size: 204 36 474 210 172 59
Fragments arranged by size:
474 210 204 172 59 36
SfcI C'TryA_G
Cuts at: 0 963 1155
Size: 963 192
SmlI C'TyrA_G
Cuts at: 0 511 1155
Size: 511 644
StuI AGG'CCT
Cuts at: 0 969 1082 1155
Size: 969 113 73
Fragments arranged by size:
969 113 73
StyI C'CwwG_G
Cuts at: 0 263 467 1011 1021 1155
Size: 263 204 544 10 134
Fragments arranged by size:
544 263 204 134 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 1155
Size: 724 431
TatI w'GTAC_w
Cuts at: 0 989 1155
Size: 989 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 1155
Size: 926 32 197
Fragments arranged by size:
926 197 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1155
Size: 528 627
Enzymes that do cut:
AcuI AfeI AflIII AleI AloI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbsI BglI
Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaXI
BseYI BsiHKAI Bsp1286I BspMI BstEII BstYI Bsu36I BtgI
BtsI EaeI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII
MscI MslI MspA1I PflMI PmlI PpiI PpuMI PspOMI
PvuII SfcI SmlI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AscI AseI AsiSI BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI