################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvrII BamHI BbeI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI SspBI SspI SwaI TfiI Tru9I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1315 GACGTC GACGT^C AccIII 1304 TCCGGA T^CCGGA AcsI 1348 GAATTC [GA]^AATT[CT] Alw44I 916 GTGCAC G^TGCAC AlwNI 237 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 916 GTGCAC G^TGCAC ApoI 1348 GAATTC [GA]^AATT[CT] AspI 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 107 TGCGCA TGC^GCA BanI 7 GGCACC G^G[CT][GA]CC BbrPI 685 CACGTG CAC^GTG BglI 954 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 685 CACGTG [CT]AC^GT[GA] BsaBI 1105 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1304 TCCGGA [AT]^CCGG[AT] BseAI 1304 TCCGGA T^CCGGA BsmBI 606 CGTCTC CGTCTC(1/5) BspEI 1304 TCCGGA T^CCGGA BsrBI 580 GAGCGG CCGCTC(-3/-3) BsrFI 866 ACCGGC [GA]^CCGG[CT] BstAPI 99 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1038 GGTGACC G^GT[ACGT]ACC BstPI 1038 GGTGACC G^GT[ACGT]ACC BstXI 464 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 866 ACCGGC [GA]^CCGG[CT] DrdI 565 GACAACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 595 CTCTTC CTCTTC(1/4) EcoO65I 1038 GGTGACC G^GT[ACGT]ACC EcoRI 1348 GAATTC G^AATTC FspI 107 TGCGCA TGC^GCA HaeII 1384 AGCGCC [GA]GCGC^[CT] Ksp632I 595 CTCTTC CTCTTC(1/4) KspI 51 CCGCGG CCGC^GG MamI 1105 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1304 TCCGGA T^CCGGA MslI 723 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 110 GCATGT [GA]CATG^[CT] PflFI 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 685 CACGTG CAC^GTG PmlI 685 CACGTG CAC^GTG SacII 51 CCGCGG CCGC^GG SbfI 210 CCTGCAGG CCTGCA^GG SpeI 834 ACTAGT A^CTAGT Sse8387I 210 CCTGCAGG CCTGCA^GG StuI 1762 AGGCCT AGG^CCT Tth111I 364 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 869 GGCGTC G[GA]^CG[CT]C 1315 GACGTC ApaI 659 GGGCCC GGGCC^C 1652 GGGCCC AspHI 916 GTGCAC G[AT]GC[AT]^C 1273 GAGCAC BalI 1031 TGGCCA TGG^CCA 1329 TGGCCA BbvCI 633 CCTCAGC CCTCAGC(-5/-2) 1380 CCTCAGC BcgI 347 GCAGCCTAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1481 GCATGGGCTTCG BfaI 319 CTAG C^TAG 835 CTAG BlpI 289 GCTGAGC GC^T[ACGT]AGC 1080 GCTGAGC Bpu1102I 289 GCTGAGC GC^T[ACGT]AGC 1080 GCTGAGC BsaHI 869 GGCGTC G[GA]^CG[CT]C 1315 GACGTC BsiHKAI 916 GTGCAC G[AT]GC[AT]^C 1273 GAGCAC BspHI 119 TCATGA T^CATGA 626 TCATGA BssSI 1185 CACGAG CACGAG(-5/-1) 1264 CTCGTG CelII 289 GCTGAGC GC^T[ACGT]AGC 1080 GCTGAGC EcoNI 18 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 519 CCTGTTTCAGG EcoT14I 464 CCATGG C^C[AT][AT]GG 1034 CCATGG Hin1I 869 GGCGTC G[GA]^CG[CT]C 1315 GACGTC MaeI 319 CTAG C^TAG 835 CTAG MluNI 1031 TGGCCA TGG^CCA 1329 TGGCCA MscI 1031 TGGCCA TGG^CCA 1329 TGGCCA NcoI 464 CCATGG C^CATGG 1034 CCATGG RcaI 119 TCATGA T^CATGA 626 TCATGA SexAI 230 ACCTGGT A^CC[AT]GGT 929 ACCTGGT SmlI 772 CTCAAG C^T[CT][GA]AG 922 CTCAAG StyI 464 CCATGG C^C[AT][AT]GG 1034 CCATGG Tsp509I 205 AATT ^AATT 1349 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII