(Circular) (Six-base) MAPSORT of: pFN21AE2026.seq Check: 7778 from: 1 to: 6532 pFN21AE2026 6532 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2209 2389 2209 Size: 180 6352 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3324 4342 278 Size: 53 83 186 936 1788 1018 2468 Fragments arranged by size: 2468 1788 1018 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 4659 5032 1563 Size: 3096 373 3063 Fragments arranged by size: 3096 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 3712 4595 5979 6247 1632 Size: 701 1379 883 1384 268 1917 Fragments arranged by size: 1917 1384 1379 883 701 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 5431 6408 1203 Size: 4228 977 1327 Fragments arranged by size: 4228 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3539 1184 Size: 2355 4177 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 3290 Size: 32 6500 AlwNI CAG_nnn'CTG Cuts at: 2247 4275 5847 2247 Size: 2028 1572 2932 Fragments arranged by size: 2932 2028 1572 ApaI G_GGCC'C Cuts at: 2668 3661 2668 Size: 993 5539 ApaLI G'TGCA_C Cuts at: 2921 4589 5745 2921 Size: 1668 1156 3708 Fragments arranged by size: 3708 1668 1156 ApoI r'AATT_y Cuts at: 1493 3353 3841 4067 4219 6328 1493 Size: 1860 488 226 152 2109 1697 Fragments arranged by size: 2109 1860 1697 488 226 152 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 3853 5308 618 Size: 325 217 627 225 1841 1455 1842 Fragments arranged by size: 1842 1841 1455 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 2469 2668 3661 3851 1391 Size: 1078 199 993 190 4072 Fragments arranged by size: 4072 1078 993 199 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5561 BbvCI CC'TCA_GC Cuts at: 2639 3386 2639 Size: 747 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2341 2375 3475 3509 4723 4757 1903 Size: 34 404 34 1100 34 1214 34 3678 Fragments arranged by size: 3678 1214 1100 404 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 5634 4424 Size: 1210 5322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2965 5143 136 Size: 107 122 71 631 605 1293 2178 1525 Fragments arranged by size: 2178 1525 1293 631 605 122 107 71 BglII A'GATC_T Cuts at: 6527 6527 Size: 6532 BlpI GC'TnA_GC Cuts at: 2295 3086 3949 2295 Size: 791 863 4878 Fragments arranged by size: 4878 863 791 Bme1580I G_kGCm'C Cuts at: 1790 2668 2925 3661 4593 5749 1790 Size: 878 257 736 932 1156 2573 Fragments arranged by size: 2573 1156 932 878 736 257 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 3181 3253 3535 2524 Size: 657 72 282 5521 Fragments arranged by size: 5521 657 282 72 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 2603 3047 3245 3448 3695 3748 3756 Size: 1244 120 444 198 203 247 53 8 Cuts at: 3756 5192 1239 Size: 1436 2579 Fragments arranged by size: 2579 1436 1244 444 247 203 198 120 53 8 Bpu10I CC'TnA_GC Cuts at: 2639 2753 3386 2639 Size: 114 633 5785 Fragments arranged by size: 5785 633 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 2912 4657 5522 5820 2762 Size: 150 1745 865 298 3474 Fragments arranged by size: 3474 1745 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2777 2801 2834 5195 882 Size: 617 1278 24 33 2361 2219 Fragments arranged by size: 2361 2219 1278 617 33 24 BsaAI yAC'GTr Cuts at: 493 1204 2692 4323 493 Size: 711 1488 1631 2702 Fragments arranged by size: 2702 1631 1488 711 BsaBI GATnn'nnATC Cuts at: 3114 6526 3114 Size: 3412 3120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2875 3321 4339 4721 Size: 53 83 186 936 1342 446 1018 382 Cuts at: 4721 5356 275 Size: 635 1451 Fragments arranged by size: 1451 1342 1018 936 635 446 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3309 4965 5363 5637 5784 6195 3309 Size: 1656 398 274 147 411 3646 Fragments arranged by size: 3646 1656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 2237 2267 3290 Size: 30 165 30 729 30 75 30 1023 Cuts at: 3290 3320 1178 Size: 30 4390 Fragments arranged by size: 4390 1023 729 165 75 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2140 3044 3242 3452 3800 3803 1186 Size: 874 80 904 198 210 348 3 3915 Fragments arranged by size: 3915 904 874 348 210 198 80 3 BseYI C'CCAG_C Cuts at: 2263 2974 5735 6231 2263 Size: 711 2761 496 2564 Fragments arranged by size: 2761 2564 711 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2020 2545 2645 3669 1163 Size: 573 284 525 100 1024 4026 Fragments arranged by size: 4026 1024 573 525 284 100 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2925 3282 4593 4678 5749 2925 Size: 357 1311 85 1071 3708 Fragments arranged by size: 3708 1311 1071 357 85 BsmI GAATG_Cn' Cuts at: 4043 4136 4043 Size: 93 6439 BsmBI CGTCTCn'nnnn_ Cuts at: 2617 2617 Size: 6532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2469 2668 2925 3282 3661 3851 4593 Size: 399 679 199 257 357 379 190 742 Cuts at: 4593 4678 5749 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 399 379 357 257 199 190 85 BspEI T'CCGG_A Cuts at: 3309 6195 3309 Size: 2886 3646 BspHI T'CATG_A Cuts at: 2124 2631 4421 6151 2124 Size: 507 1790 1730 2505 Fragments arranged by size: 2505 1790 1730 507 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2209 2389 3090 3887 844 Size: 1365 180 701 797 3489 Fragments arranged by size: 3489 1365 797 701 180 BsrBI CCG'CTC Cuts at: 1394 1823 2587 4419 1394 Size: 429 764 1832 3507 Fragments arranged by size: 3507 1832 764 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5027 5201 65 Size: 1183 3779 174 1396 Fragments arranged by size: 3779 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2871 5176 5363 1700 Size: 110 1061 2305 187 2869 Fragments arranged by size: 2869 2305 1061 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6532 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 3190 3269 4592 5604 3190 Size: 79 1323 1012 4118 Fragments arranged by size: 4118 1323 1012 79 BstAPI GCAn_nnn'nTGC Cuts at: 794 2110 794 Size: 1316 5216 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 3043 1184 Size: 1859 4673 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 6532 BstYI r'GATC_y Cuts at: 1972 2250 2520 3777 3862 4229 4614 4631 6083 Size: 278 270 1257 85 367 385 17 1452 Cuts at: 6083 6169 6181 6527 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 278 270 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2056 2469 3039 3367 513 Size: 552 939 52 413 570 328 3678 Fragments arranged by size: 3678 939 570 552 413 328 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 2745 2954 3544 3626 4048 4842 4862 2745 Size: 209 590 82 422 794 20 4415 Fragments arranged by size: 4415 794 590 422 209 82 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 3836 3836 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2576 5539 809 Size: 507 1260 2963 1802 Fragments arranged by size: 2963 1802 1260 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 3036 3056 Size: 54 1425 50 275 18 333 873 20 Cuts at: 3056 3334 3979 4868 6497 8 Size: 278 645 889 1629 43 Fragments arranged by size: 1629 1425 889 873 645 333 278 275 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 4462 1353 Size: 1253 1856 3423 Fragments arranged by size: 3423 1856 1253 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2245 2495 2711 5119 5493 5639 Size: 1064 138 656 250 216 2408 374 146 Cuts at: 5639 387 Size: 1280 Fragments arranged by size: 2408 1280 1064 656 374 250 216 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2483 2603 3047 3245 3448 3695 Size: 393 701 150 120 444 198 203 247 Cuts at: 3695 3712 3748 3756 4595 5192 5979 6247 1239 Size: 17 36 8 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 701 597 444 393 268 247 203 198 150 120 36 17 8 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2528 2027 Size: 501 6031 EcoO109I rG'GnC_Cy Cuts at: 2098 2665 2749 2932 3265 3400 3580 3657 2098 Size: 567 84 183 333 135 180 77 4973 Fragments arranged by size: 4973 567 333 183 180 135 84 77 EcoRI G'AATT_C Cuts at: 3353 3841 4219 3353 Size: 488 378 5666 Fragments arranged by size: 5666 488 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3031 3031 Size: 6532 FspI TGC'GCA Cuts at: 2114 5038 2114 Size: 2924 3608 FspAI rTGC'GCAy Cuts at: 2114 2114 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 3393 5679 1678 Size: 1715 2286 2531 Fragments arranged by size: 2531 2286 1715 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3620 3652 5173 5205 5247 Size: 32 135 32 2048 32 1521 32 42 Cuts at: 5247 5279 1373 Size: 32 2626 Fragments arranged by size: 2626 2048 1521 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3876 4122 669 Size: 988 281 1938 246 3079 Fragments arranged by size: 3079 1938 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6532 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5646 5830 1305 Size: 4341 184 2007 Fragments arranged by size: 4341 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3038 3336 10 Size: 54 1425 343 1206 298 3206 Fragments arranged by size: 3206 1425 1206 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 4490 4849 5008 518 Size: 648 1566 1758 359 159 2042 Fragments arranged by size: 2042 1758 1566 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2107 2158 2267 2410 2662 2713 2950 Size: 452 49 51 109 143 252 51 237 Cuts at: 2950 3142 3154 3948 4625 5773 6018 1606 Size: 192 12 794 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 452 252 245 237 192 143 109 51 51 49 12 NcoI C'CATG_G Cuts at: 513 1065 2004 2469 3039 513 Size: 552 939 465 570 4006 Fragments arranged by size: 4006 939 570 552 465 NdeI CA'TA_TG Cuts at: 387 387 Size: 6532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 2119 3890 5435 1132 Size: 97 890 1771 1545 2229 Fragments arranged by size: 2229 1771 1545 890 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6532 PfoI T'CCnGG_A Cuts at: 2084 2192 2580 3024 3507 2084 Size: 108 388 444 483 5109 Fragments arranged by size: 5109 483 444 388 108 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PmlI CAC'GTG Cuts at: 2692 2692 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 4601 4633 6114 6146 3290 Size: 32 1279 32 1481 32 3676 Fragments arranged by size: 3676 1481 1279 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2932 3400 2098 Size: 834 468 5230 Fragments arranged by size: 5230 834 468 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PspOMI G'GGCC_C Cuts at: 2664 3657 2664 Size: 993 5539 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3365 3397 3365 Size: 32 6500 PstI C_TGCA'G Cuts at: 830 2220 2718 2949 3153 3884 830 Size: 1390 498 231 204 731 3478 Fragments arranged by size: 3478 1390 731 498 231 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 2107 2158 2713 2950 3142 2107 Size: 51 555 237 192 5497 Fragments arranged by size: 5497 555 237 192 51 SacII CC_GC'GG Cuts at: 2059 2059 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SanDI GG'GwC_CC Cuts at: 2098 2098 Size: 6532 SbfI CC_TGCA'GG Cuts at: 2220 3884 2220 Size: 1664 4868 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SexAI A'CCwGG_T Cuts at: 2235 2934 2235 Size: 699 5833 SfcI C'TryA_G Cuts at: 826 1045 2216 2708 2714 2945 3149 3880 5015 Size: 219 1171 492 6 231 204 731 1135 Cuts at: 5015 5696 5887 826 Size: 681 191 1471 Fragments arranged by size: 1471 1171 1135 731 681 492 231 219 204 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2777 2927 4636 5537 5799 820 Size: 197 926 834 150 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 834 262 197 150 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 2839 152 Size: 2687 3845 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SspI AAT'ATT Cuts at: 5 52 4456 5 Size: 47 4404 2081 Fragments arranged by size: 4404 2081 47 StuI AGG'CCT Cuts at: 1517 3769 1517 Size: 2252 4280 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 3039 3971 513 Size: 552 939 465 570 932 3074 Fragments arranged by size: 3074 939 932 570 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 2906 3715 4559 4576 4729 4914 Size: 1255 1031 295 809 844 17 153 185 Cuts at: 4914 5359 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1031 844 809 445 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3064 4778 6490 Size: 274 80 33 51 1443 1087 1714 1712 Cuts at: 6490 96 Size: 138 Fragments arranged by size: 1714 1712 1443 1087 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2670 2702 6492 6524 2670 Size: 32 3790 32 2678 Fragments arranged by size: 3790 2678 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2372 1316 Size: 1056 5476 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3253 3365 3416 3472 3253 Size: 112 51 56 6313 Fragments arranged by size: 6313 112 56 51 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 4661 4661 Size: 6532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3322 4340 276 Size: 53 83 186 936 1788 1018 2468 Fragments arranged by size: 2468 1788 1018 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacI SapI SfiI SfoI SrfI SwaI