(Linear) (Six-base) MAPSORT of: KIEE2026.seq Check: 4005 from: 1 to: 1827 KIEE2026.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 204 384 1827 Size: 204 180 1443 Fragments arranged by size: 1443 204 180 AatII G_ACGT'C Cuts at: 0 1319 1827 Size: 1319 508 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1707 1827 Size: 328 1379 120 Fragments arranged by size: 1379 328 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1534 1827 Size: 1534 293 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1285 1317 1827 Size: 1285 32 510 Fragments arranged by size: 1285 510 32 AlwNI CAG_nnn'CTG Cuts at: 0 242 1827 Size: 242 1585 ApaI G_GGCC'C Cuts at: 0 663 1656 1827 Size: 663 993 171 Fragments arranged by size: 993 663 171 ApaLI G'TGCA_C Cuts at: 0 916 1827 Size: 916 911 ApoI r'AATT_y Cuts at: 0 1348 1827 Size: 1348 479 BanI G'GyrC_C Cuts at: 0 7 1827 Size: 7 1820 BanII G_rGCy'C Cuts at: 0 464 663 1656 1827 Size: 464 199 993 171 Fragments arranged by size: 993 464 199 171 BbvCI CC'TCA_GC Cuts at: 0 634 1381 1827 Size: 634 747 446 Fragments arranged by size: 747 634 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 336 370 1470 1504 1827 Size: 336 34 1100 34 323 Fragments arranged by size: 1100 336 323 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 960 1827 Size: 960 867 BlpI GC'TnA_GC Cuts at: 0 290 1081 1827 Size: 290 791 746 Fragments arranged by size: 791 746 290 Bme1580I G_kGCm'C Cuts at: 0 663 920 1656 1827 Size: 663 257 736 171 Fragments arranged by size: 736 663 257 171 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 519 1176 1248 1530 1827 Size: 519 657 72 282 297 Fragments arranged by size: 657 519 297 282 72 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 478 598 1042 1240 1443 1690 1743 1751 Size: 478 120 444 198 203 247 53 8 Cuts at: 1751 1827 Size: 76 Fragments arranged by size: 478 444 247 203 198 120 76 53 8 Bpu10I CC'TnA_GC Cuts at: 0 634 748 1381 1827 Size: 634 114 633 446 Fragments arranged by size: 634 633 446 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 907 1827 Size: 757 150 920 Fragments arranged by size: 920 757 150 BsaI GGTCTCn'nnnn_ Cuts at: 0 772 796 829 1827 Size: 772 24 33 998 Fragments arranged by size: 998 772 33 24 BsaAI yAC'GTr Cuts at: 0 687 1827 Size: 687 1140 BsaBI GATnn'nnATC Cuts at: 0 1109 1827 Size: 1109 718 BsaHI Gr'CG_yC Cuts at: 0 870 1316 1827 Size: 870 446 511 Fragments arranged by size: 870 511 446 BsaWI w'CCGG_w Cuts at: 0 1304 1827 Size: 1304 523 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 127 157 232 262 1285 1315 1827 Size: 127 30 75 30 1023 30 512 Fragments arranged by size: 1023 512 127 75 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 135 1039 1237 1447 1795 1798 1827 Size: 55 80 904 198 210 348 3 29 Fragments arranged by size: 904 348 210 198 80 55 29 3 BseYI C'CCAG_C Cuts at: 0 258 969 1827 Size: 258 711 858 Fragments arranged by size: 858 711 258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 540 640 1664 1827 Size: 15 525 100 1024 163 Fragments arranged by size: 1024 525 163 100 15 BsiHKAI G_wGCw'C Cuts at: 0 920 1277 1827 Size: 920 357 550 Fragments arranged by size: 920 550 357 BsmBI CGTCTCn'nnnn_ Cuts at: 0 612 1827 Size: 612 1215 Bsp1286I G_dGCh'C Cuts at: 0 464 663 920 1277 1656 1827 Size: 464 199 257 357 379 171 Fragments arranged by size: 464 379 357 257 199 171 BspEI T'CCGG_A Cuts at: 0 1304 1827 Size: 1304 523 BspHI T'CATG_A Cuts at: 0 119 626 1827 Size: 119 507 1201 Fragments arranged by size: 1201 507 119 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 204 384 1085 1827 Size: 204 180 701 742 Fragments arranged by size: 742 701 204 180 BsrBI CCG'CTC Cuts at: 0 582 1827 Size: 582 1245 BsrFI r'CCGG_y Cuts at: 0 866 1827 Size: 866 961 BssSI C'ACGA_G Cuts at: 0 1185 1264 1827 Size: 1185 79 563 Fragments arranged by size: 1185 563 79 BstAPI GCAn_nnn'nTGC Cuts at: 0 105 1827 Size: 105 1722 BstEII G'GTnAC_C Cuts at: 0 1038 1827 Size: 1038 789 BstXI CCAn_nnnn'nTGG Cuts at: 0 471 1827 Size: 471 1356 BstYI r'GATC_y Cuts at: 0 245 515 1772 1827 Size: 245 270 1257 55 Fragments arranged by size: 1257 270 245 55 BtgI C'CryG_G Cuts at: 0 51 464 1034 1362 1827 Size: 51 413 570 328 465 Fragments arranged by size: 570 465 413 328 51 BtsI GCAGTG_nn' Cuts at: 0 740 949 1539 1621 1827 Size: 740 209 590 82 206 Fragments arranged by size: 740 590 209 206 82 DrdI GACnn_nn'nnGTC Cuts at: 0 571 1827 Size: 571 1256 EaeI y'GGCC_r Cuts at: 0 158 1031 1051 1329 1827 Size: 158 873 20 278 498 Fragments arranged by size: 873 498 278 158 20 EarI CTCTTCn'nnn_ Cuts at: 0 601 1827 Size: 601 1226 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 240 490 706 1827 Size: 240 250 216 1121 Fragments arranged by size: 1121 250 240 216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 478 598 1042 1240 1443 1690 1707 Size: 328 150 120 444 198 203 247 17 Cuts at: 1707 1743 1751 1827 Size: 36 8 76 Fragments arranged by size: 444 328 247 203 198 150 120 76 36 17 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 22 523 1827 Size: 22 501 1304 Fragments arranged by size: 1304 501 22 EcoO109I rG'GnC_Cy Cuts at: 0 93 660 744 927 1260 1395 1575 1652 Size: 93 567 84 183 333 135 180 77 Cuts at: 1652 1827 Size: 175 Fragments arranged by size: 567 333 183 180 175 135 93 84 77 EcoRI G'AATT_C Cuts at: 0 1348 1827 Size: 1348 479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1026 1827 Size: 1026 801 FspI TGC'GCA Cuts at: 0 109 1827 Size: 109 1718 FspAI rTGC'GCAy Cuts at: 0 109 1827 Size: 109 1718 HaeII r_GCGC'y Cuts at: 0 1388 1827 Size: 1388 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1615 1647 1827 Size: 1615 32 180 Fragments arranged by size: 1615 180 32 MscI TGG'CCA Cuts at: 0 1033 1331 1827 Size: 1033 298 496 Fragments arranged by size: 1033 496 298 MslI CAynn'nnrTG Cuts at: 0 727 1827 Size: 727 1100 MspA1I CmG'CkG Cuts at: 0 53 102 153 262 405 657 708 945 Size: 53 49 51 109 143 252 51 237 Cuts at: 945 1137 1149 1827 Size: 192 12 678 Fragments arranged by size: 678 252 237 192 143 109 53 51 51 49 12 NcoI C'CATG_G Cuts at: 0 464 1034 1827 Size: 464 570 793 Fragments arranged by size: 793 570 464 NspI r_CATG'y Cuts at: 0 114 1827 Size: 114 1713 PfoI T'CCnGG_A Cuts at: 0 79 187 575 1019 1502 1827 Size: 79 108 388 444 483 325 Fragments arranged by size: 483 444 388 325 108 79 PmlI CAC'GTG Cuts at: 0 687 1827 Size: 687 1140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1285 1317 1827 Size: 1285 32 510 Fragments arranged by size: 1285 510 32 PpuMI rG'GwC_Cy Cuts at: 0 93 927 1395 1827 Size: 93 834 468 432 Fragments arranged by size: 834 468 432 93 PspOMI G'GGCC_C Cuts at: 0 659 1652 1827 Size: 659 993 175 Fragments arranged by size: 993 659 175 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1360 1392 1827 Size: 1360 32 435 Fragments arranged by size: 1360 435 32 PstI C_TGCA'G Cuts at: 0 215 713 944 1148 1827 Size: 215 498 231 204 679 Fragments arranged by size: 679 498 231 215 204 PvuII CAG'CTG Cuts at: 0 102 153 708 945 1137 1827 Size: 102 51 555 237 192 690 Fragments arranged by size: 690 555 237 192 102 51 SacII CC_GC'GG Cuts at: 0 54 1827 Size: 54 1773 SanDI GG'GwC_CC Cuts at: 0 93 1827 Size: 93 1734 SbfI CC_TGCA'GG Cuts at: 0 215 1827 Size: 215 1612 SexAI A'CCwGG_T Cuts at: 0 230 929 1827 Size: 230 699 898 Fragments arranged by size: 898 699 230 SfcI C'TryA_G Cuts at: 0 211 703 709 940 1144 1827 Size: 211 492 6 231 204 683 Fragments arranged by size: 683 492 231 211 204 6 SmlI C'TyrA_G Cuts at: 0 772 922 1827 Size: 772 150 905 Fragments arranged by size: 905 772 150 SpeI A'CTAG_T Cuts at: 0 834 1827 Size: 834 993 StuI AGG'CCT Cuts at: 0 1764 1827 Size: 1764 63 StyI C'CwwG_G Cuts at: 0 464 1034 1827 Size: 464 570 793 Fragments arranged by size: 793 570 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 606 901 1710 1827 Size: 606 295 809 117 Fragments arranged by size: 809 606 295 117 TatI w'GTAC_w Cuts at: 0 1059 1827 Size: 1059 768 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 665 697 1827 Size: 665 32 1130 Fragments arranged by size: 1130 665 32 Tth111I GACn'n_nGTC Cuts at: 0 367 1827 Size: 367 1460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1248 1360 1411 1467 1827 Size: 1248 112 51 56 360 Fragments arranged by size: 1248 360 112 56 51 ZraI GAC'GTC Cuts at: 0 1317 1827 Size: 1317 510 Enzymes that do cut: AarI AatII AcuI AlfI AloI AlwNI ApaI ApaLI ApoI BanI BanII BbvCI BcgI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I MscI MslI MspA1I NcoI NspI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacII SanDI SbfI SexAI SfcI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BsiEI BsiWI BsmI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoRV FseI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PshAI PsiI PspXI PvuI RsrII SacI SalI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI