Restriction map for
pFN21AB8483
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BamHI BbrPI
BclI BfaI BfrI BglII BlnI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MaeI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SalI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1475 TCCGGA T^CCGGA
AcsI 142 GAATTC [GA]^AATT[CT]
AhdI 2371 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 142 GAATTC [GA]^AATT[CT]
AspEI 2371 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 974 TGGCCA TGG^CCA
BbeI 2698 GGCGCC GGCGC^C
BbvCI 1526 CCTCAGC CCTCAGC(-5/-2)
BcgI 2151 GCAGGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 677 GGATAC GTATCC(6/5)
BsaAI 1922 CACGTA [CT]AC^GT[GA]
BsaBI 510 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 783 GGTCTC GGTCTC(1/5)
BsaWI 1475 TCCGGA [AT]^CCGG[AT]
BseAI 1475 TCCGGA T^CCGGA
BsmBI 204 GAGACG CGTCTC(1/5)
BsmI 531 GAATGC GAATGC(1/-1)
BspEI 1475 TCCGGA T^CCGGA
BssSI 1197 CTCGTG CACGAG(-5/-1)
DrdI 747 GACGGGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2021 CGGCCG C^GGCCG
Eam1105I 2371 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2021 CGGCCG C^GGCCG
Eco52I 2021 CGGCCG C^GGCCG
EcoNI 1719 CCTCCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 142 GAATTC G^AATTC
EcoRV 2623 GATATC GAT^ATC
KasI 2698 GGCGCC G^GCGCC
KspI 395 CCGCGG CCGC^GG
MamI 510 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 974 TGGCCA TGG^CCA
MroI 1475 TCCGGA T^CCGGA
MscI 974 TGGCCA TGG^CCA
MseI 1559 TTAA T^TAA
MslI 690 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2698 GGCGCC GG^CGCC
PvuI 920 CGATCG CGAT^CG
SacII 395 CCGCGG CCGC^GG
SbfI 2691 CCTGCAGG CCTGCA^GG
SfiI 2343 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2698 GGCGCC GGC^GCC
SmlI 100 CTTGAG C^T[CT][GA]AG
Sse8387I 2691 CCTGCAGG CCTGCA^GG
Tru9I 1559 TTAA T^TAA
Tsp509I 143 AATT ^AATT
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 296 GACGCC G[GA]^CG[CT]C
2698 GGCGCC
AlwI 238 GATCC GGATC(4/5)
2460 GGATC
BcnI 1263 CCCGG CC^[GC]GG
2501 CCCGG
BglI 2326 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2344 GCCAGCAAGGC
BsaHI 296 GACGCC G[GA]^CG[CT]C
2698 GGCGCC
BsiEI 920 CGATCG CG[GA][CT]^CG
2021 CGGCCG
BspHI 1514 TCATGA T^CATGA
1544 TCATGA
BspMI 29 ACCTGC ACCTGC(4/8)
780 GCAGGT
BstAPI 409 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1488 GCACACAGTGC
BstYI 237 AGATCC [GA]^GATC[CT]
2460 GGATCT
DraIII 480 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1489 CACACAGTG
Hin1I 296 GACGCC G[GA]^CG[CT]C
2698 GGCGCC
MaeII 1923 ACGT A^CGT
2372 ACGT
MflI 237 AGATCC [GA]^GATC[CT]
2460 GGATCT
NciI 1263 CCCGG CC^[GC]GG
2501 CCCGG
NcoI 404 CCATGG C^CATGG
826 CCATGG
NspI 128 GCATGT [GA]CATG^[CT]
543 GCATGT
RcaI 1514 TCATGA T^CATGA
1544 TCATGA
ScaI 962 AGTACT AGT^ACT
2561 AGTACT
TaqI 667 TCGA T^CGA
1805 TCGA
TthHB8I 667 TCGA T^CGA
1805 TCGA
XhoII 237 AGATCC [GA]^GATC[CT]
2460 GGATCT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII AviII
BanI BanII BbsI BbvI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I TspRI XcmI