Restriction map for
pFN21AB8483
(Linear) (Six-base) MAPSORT of: KIBB8483.seq Check: 9438 from: 1 to: 2742
KIBB8483.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 38 2742
Size: 38 2704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1723 2742
Size: 587 1136 1019
Fragments arranged by size:
1136 1019 587
AhdI GACnn_n'nnGTC
Cuts at: 0 2376 2742
Size: 2376 366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1747 1811 2742
Size: 1747 64 931
Fragments arranged by size:
1747 931 64
AlwNI CAG_nnn'CTG
Cuts at: 0 1395 1611 2286 2742
Size: 1395 216 675 456
Fragments arranged by size:
1395 675 456 216
ApaI G_GGCC'C
Cuts at: 0 23 2427 2526 2742
Size: 23 2404 99 216
Fragments arranged by size:
2404 216 99 23
ApoI r'AATT_y
Cuts at: 0 142 2742
Size: 142 2600
AvaI C'yCGr_G
Cuts at: 0 466 936 1135 2525 2742
Size: 466 470 199 1390 217
Fragments arranged by size:
1390 470 466 217 199
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 670 703 2742
Size: 670 33 2039
Fragments arranged by size:
2039 670 33
BanI G'GyrC_C
Cuts at: 0 495 1098 1964 2530 2698 2742
Size: 495 603 866 566 168 44
Fragments arranged by size:
866 603 566 495 168 44
BanII G_rGCy'C
Cuts at: 0 23 292 703 886 2427 2526 2591 2742
Size: 23 269 411 183 1541 99 65 151
Fragments arranged by size:
1541 411 269 183 151 99 65 23
BbeI G_GCGC'C
Cuts at: 0 2702 2742
Size: 2702 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 923 1030 1819 2742
Size: 923 107 789 923
Fragments arranged by size:
923 923 789 107
BbvCI CC'TCA_GC
Cuts at: 0 1527 2742
Size: 1527 1215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2140 2174 2742
Size: 2140 34 568
Fragments arranged by size:
2140 568 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 671 2742
Size: 671 2071
BglI GCCn_nnn'nGGC
Cuts at: 0 2332 2350 2742
Size: 2332 18 392
Fragments arranged by size:
2332 392 18
BlpI GC'TnA_GC
Cuts at: 0 286 536 1145 1163 2030 2291 2539 2742
Size: 286 250 609 18 867 261 248 203
Fragments arranged by size:
867 609 286 261 250 248 203 18
Bme1580I G_kGCm'C
Cuts at: 0 23 1103 2420 2427 2526 2742
Size: 23 1080 1317 7 99 216
Fragments arranged by size:
1317 1080 216 99 23 7
BmgBI CAC'GTC
Cuts at: 0 2373 2742
Size: 2373 369
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 2742
Size: 477 2265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 2742
Size: 369 2373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 581 703 1096 1417 1633 1708 2164
Size: 55 526 122 393 321 216 75 456
Cuts at: 2164 2272 2366 2605 2742
Size: 108 94 239 137
Fragments arranged by size:
526 456 393 321 239 216 137 122
108 94 75 55
Bpu10I CC'TnA_GC
Cuts at: 0 769 1527 1930 2742
Size: 769 758 403 812
Fragments arranged by size:
812 769 758 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 2742
Size: 121 2621
BsaI GGTCTCn'nnnn_
Cuts at: 0 789 2742
Size: 789 1953
BsaAI yAC'GTr
Cuts at: 0 1924 2742
Size: 1924 818
BsaBI GATnn'nnATC
Cuts at: 0 514 2742
Size: 514 2228
BsaHI Gr'CG_yC
Cuts at: 0 297 2699 2742
Size: 297 2402 43
Fragments arranged by size:
2402 297 43
BsaWI w'CCGG_w
Cuts at: 0 1475 2742
Size: 1475 1267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1457 1487 2742
Size: 1457 30 1255
Fragments arranged by size:
1457 1255 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1153 1399 1420 1648 1681 1705 1780
Size: 130 1023 246 21 228 33 24 75
Cuts at: 1780 1846 1861 1900 1930 1963 2011 2269 2323
Size: 66 15 39 30 33 48 258 54
Cuts at: 2323 2742
Size: 419
Fragments arranged by size:
1023 419 258 246 228 130 75 66
54 48 39 33 33 30 24 21
15
BseYI C'CCAG_C
Cuts at: 0 551 1449 2710 2742
Size: 551 898 1261 32
Fragments arranged by size:
1261 898 551 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1471 1516 2395 2742
Size: 401 1070 45 879 347
Fragments arranged by size:
1070 879 401 347 45
BsiEI CG_ry'CG
Cuts at: 0 923 2024 2742
Size: 923 1101 718
Fragments arranged by size:
1101 923 718
BsiHKAI G_wGCw'C
Cuts at: 0 292 703 2591 2742
Size: 292 411 1888 151
Fragments arranged by size:
1888 411 292 151
BsmI GAATG_Cn'
Cuts at: 0 537 2742
Size: 537 2205
BsmBI CGTCTCn'nnnn_
Cuts at: 0 198 2742
Size: 198 2544
Bsp1286I G_dGCh'C
Cuts at: 0 23 292 703 886 1103 2420 2427 2526
Size: 23 269 411 183 217 1317 7 99
Cuts at: 2526 2591 2742
Size: 65 151
Fragments arranged by size:
1317 411 269 217 183 151 99 65
23 7
BspEI T'CCGG_A
Cuts at: 0 1475 2742
Size: 1475 1267
BspHI T'CATG_A
Cuts at: 0 1514 1544 2742
Size: 1514 30 1198
Fragments arranged by size:
1514 1198 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 771 2742
Size: 38 733 1971
Fragments arranged by size:
1971 733 38
BsrBI CCG'CTC
Cuts at: 0 837 1602 1746 1836 1959 2007 2061 2076
Size: 837 765 144 90 123 48 54 15
Cuts at: 2076 2091 2106 2121 2175 2205 2295 2538 2742
Size: 15 15 15 54 30 90 243 204
Fragments arranged by size:
837 765 243 204 144 123 90 90
54 54 48 30 15 15 15 15
BssSI C'ACGA_G
Cuts at: 0 1197 2742
Size: 1197 1545
BstAPI GCAn_nnn'nTGC
Cuts at: 0 415 1494 2742
Size: 415 1079 1248
Fragments arranged by size:
1248 1079 415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 681 1111 1312 1461 1881 2718 2742
Size: 411 270 430 201 149 420 837 24
Fragments arranged by size:
837 430 420 411 270 201 149 24
BstYI r'GATC_y
Cuts at: 0 237 2460 2742
Size: 237 2223 282
Fragments arranged by size:
2223 282 237
BtgI C'CryG_G
Cuts at: 0 395 404 796 826 878 2742
Size: 395 9 392 30 52 1864
Fragments arranged by size:
1864 395 392 52 30 9
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2597 2632 2742
Size: 2597 35 110
Fragments arranged by size:
2597 110 35
DraIII CAC_nnn'GTG
Cuts at: 0 485 1494 2742
Size: 485 1009 1248
Fragments arranged by size:
1248 1009 485
DrdI GACnn_nn'nnGTC
Cuts at: 0 753 2742
Size: 753 1989
EaeI y'GGCC_r
Cuts at: 0 974 1050 1871 2021 2742
Size: 974 76 821 150 721
Fragments arranged by size:
974 821 721 150 76
EagI C'GGCC_G
Cuts at: 0 2021 2742
Size: 2021 721
EarI CTCTTCn'nnn_
Cuts at: 0 196 1028 1907 1949 1988 2267 2629 2742
Size: 196 832 879 42 39 279 362 113
Fragments arranged by size:
879 832 362 279 196 113 42 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1641 2742
Size: 1641 1101
EcoICRI GAG'CTC
Cuts at: 0 290 701 2589 2742
Size: 290 411 1888 153
Fragments arranged by size:
1888 411 290 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 581 587 703 1096 1417 1633 1708
Size: 55 526 6 116 393 321 216 75
Cuts at: 1708 1723 2164 2272 2366 2605 2742
Size: 15 441 108 94 239 137
Fragments arranged by size:
526 441 393 321 239 216 137 116
108 94 75 55 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1723 2742
Size: 1723 1019
EcoO109I rG'GnC_Cy
Cuts at: 0 19 723 1173 2214 2352 2423 2424 2706
Size: 19 704 450 1041 138 71 1 282
Cuts at: 2706 2742
Size: 36
Fragments arranged by size:
1041 704 450 282 138 71 36 19
1
EcoRI G'AATT_C
Cuts at: 0 142 2742
Size: 142 2600
EcoRV GAT'ATC
Cuts at: 0 2625 2742
Size: 2625 117
FspI TGC'GCA
Cuts at: 0 779 1579 1651 2742
Size: 779 800 72 1091
Fragments arranged by size:
1091 800 779 72
HaeII r_GCGC'y
Cuts at: 0 528 1611 2702 2742
Size: 528 1083 1091 40
Fragments arranged by size:
1091 1083 528 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 634 666 853 885 886 918
Size: 227 32 375 32 187 32 1 32
Cuts at: 918 2362 2394 2742
Size: 1444 32 348
Fragments arranged by size:
1444 375 348 227 187 32 32 32
32 32 1
KasI G'GCGC_C
Cuts at: 0 2698 2742
Size: 2698 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2742
Size: 640 2102
MscI TGG'CCA
Cuts at: 0 976 2742
Size: 976 1766
MslI CAynn'nnrTG
Cuts at: 0 694 2742
Size: 694 2048
MspA1I CmG'CkG
Cuts at: 0 285 391 397 492 1042 1162 1647 2049
Size: 285 106 6 95 550 120 485 402
Cuts at: 2049 2142 2238 2301 2742
Size: 93 96 63 441
Fragments arranged by size:
550 485 441 402 285 120 106 96
95 93 63 6
NarI GG'CG_CC
Cuts at: 0 2699 2742
Size: 2699 43
NcoI C'CATG_G
Cuts at: 0 404 826 2742
Size: 404 422 1916
Fragments arranged by size:
1916 422 404
NspI r_CATG'y
Cuts at: 0 132 547 2742
Size: 132 415 2195
Fragments arranged by size:
2195 415 132
PasI CC'CwG_GG
Cuts at: 0 16 624 2742
Size: 16 608 2118
Fragments arranged by size:
2118 608 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 2742
Size: 115 32 2595
Fragments arranged by size:
2595 115 32
PpuMI rG'GwC_Cy
Cuts at: 0 723 1173 2706 2742
Size: 723 450 1533 36
Fragments arranged by size:
1533 723 450 36
PspOMI G'GGCC_C
Cuts at: 0 19 2423 2522 2742
Size: 19 2404 99 220
Fragments arranged by size:
2404 220 99 19
PstI C_TGCA'G
Cuts at: 0 390 1254 1281 1415 1736 1943 1982 2201
Size: 390 864 27 134 321 207 39 219
Cuts at: 2201 2228 2243 2306 2696 2742
Size: 27 15 63 390 46
Fragments arranged by size:
864 390 390 321 219 207 134 63
46 39 27 27 15
PvuI CG_AT'CG
Cuts at: 0 923 2742
Size: 923 1819
PvuII CAG'CTG
Cuts at: 0 285 391 492 1042 1162 1647 2142 2238
Size: 285 106 101 550 120 485 495 96
Cuts at: 2238 2301 2742
Size: 63 441
Fragments arranged by size:
550 495 485 441 285 120 106 101
96 63
SacI G_AGCT'C
Cuts at: 0 292 703 2591 2742
Size: 292 411 1888 151
Fragments arranged by size:
1888 411 292 151
SacII CC_GC'GG
Cuts at: 0 398 2742
Size: 398 2344
SanDI GG'GwC_CC
Cuts at: 0 2706 2742
Size: 2706 36
SapI GCTCTTCn'nnn_
Cuts at: 0 1028 1949 2267 2629 2742
Size: 1028 921 318 362 113
Fragments arranged by size:
1028 921 362 318 113
SbfI CC_TGCA'GG
Cuts at: 0 2696 2742
Size: 2696 46
ScaI AGT'ACT
Cuts at: 0 964 2563 2742
Size: 964 1599 179
Fragments arranged by size:
1599 964 179
SfcI C'TryA_G
Cuts at: 0 332 386 1250 1277 1411 1732 1939 1978
Size: 332 54 864 27 134 321 207 39
Cuts at: 1978 2197 2224 2239 2302 2692 2724 2742
Size: 219 27 15 63 390 32 18
Fragments arranged by size:
864 390 332 321 219 207 134 63
54 39 32 27 27 18 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2350 2742
Size: 2350 392
SfoI GGC'GCC
Cuts at: 0 2700 2742
Size: 2700 42
SmlI C'TyrA_G
Cuts at: 0 100 2742
Size: 100 2642
StyI C'CwwG_G
Cuts at: 0 404 728 826 1589 1826 2327 2742
Size: 404 324 98 763 237 501 415
Fragments arranged by size:
763 501 415 404 324 237 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 496 565 1251 2585 2742
Size: 444 52 69 686 1334 157
Fragments arranged by size:
1334 686 444 157 69 52
TatI w'GTAC_w
Cuts at: 0 962 2561 2742
Size: 962 1599 181
Fragments arranged by size:
1599 962 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 2742
Size: 79 32 2631
Fragments arranged by size:
2631 79 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 824 2607 2742
Size: 30 794 1783 135
Fragments arranged by size:
1783 794 135 30
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BssSI BstAPI BstXI BstYI BtgI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PpiI PpuMI PspOMI PstI PvuI PvuII SacI
SacII SanDI SapI SbfI ScaI SfcI SfiI SfoI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BclI BfrBI BglII BmtI BsiWI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
DraI FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspXI PsrI RsrII SalI SexAI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI