Restriction map for
pFN21AE6253
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 52 GGTACC G^GTACC
AccI 97 GTCTAC GT^[AC][GT]AC
AciI 70 GCGG CCGC(-3/-1)
AcsI 617 AAATTT [GA]^AATT[CT]
AfaI 53 GTAC GT^AC
ApoI 617 AAATTT [GA]^AATT[CT]
Asp718I 52 GGTACC G^GTACC
BanI 52 GGTACC G^G[CT][GA]CC
BanII 498 GGGCTC G[GA]GC[CT]^C
BbvI 175 GCAGC GCAGC(8/12)
BciVI 350 GGATAC GTATCC(6/5)
BfaI 397 CTAG C^TAG
BmyI 498 GGGCTC G[AGT]GC[ACT]^C
BsaWI 477 ACCGGA [AT]^CCGG[AT]
BsmAI 522 GTCTC GTCTC(1/5)
BsmI 171 GAATGC GAATGC(1/-1)
Bsp1286I 498 GGGCTC G[AGT]GC[ACT]^C
BssKI 58 CCAGG ^CC[ACGT]GG
BstF5I 159 CATCC GGATG(2/0)
BstNI 58 CCAGG CC^[AT]GG
BstYI 164 AGATCC [GA]^GATC[CT]
DsaI 48 CCATGG C^C[GA][CT]GG
EcoRII 58 CCAGG ^CC[AT]GG
EcoT14I 48 CCATGG C^C[AT][AT]GG
FauI 70 GCGGG CCCGC(4/6)
Fnu4HI 175 GCAGC GC^[ACGT]GC
FokI 159 CATCC GGATG(9/13)
HapII 478 CCGG C^CGG
HpaII 478 CCGG C^CGG
ItaI 175 GCAGC GC^[ACGT]GC
KpnI 52 GGTACC GGTAC^C
MaeI 397 CTAG C^TAG
MfeI 389 CAATTG C^AATTG
MflI 164 AGATCC [GA]^GATC[CT]
MslI 573 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 478 CCGG C^CGG
MunI 389 CAATTG C^AATTG
MvaI 58 CCAGG CC^[AT]GG
MwoI 385 GCTACAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 48 CCATGG C^CATGG
PleI 472 GAGTC GAGTC(4/5)
RsaI 53 GTAC GT^AC
SapI 79 GAAGAGC GCTCTTC(1/4)
ScrFI 58 CCAGG CC^[ACGT]GG
SfcI 297 CTGTAG C^T[GA][CT]AG
SpeI 396 ACTAGT A^CTAGT
StyI 48 CCATGG C^C[AT][AT]GG
TseI 175 GCAGC G^C[AT]GC
XcmI 102 CCAGCCCATAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 164 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 29 AGCT AG^CT
83 AGCT
AlwI 165 GATCC GGATC(4/5)
115 GGATC
AlwNI 38 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
163 CAGATCCTG
AvaII 73 GGACC G^G[AT]CC
480 GGACC
BsaJI 48 CCATGG C^C[ACGT][ACGT]GG
57 CCCAGG
BsmFI 72 GGGAC GGGAC(10/14)
403 GGGAC
BsrI 380 CCAGT ACTGG(1/-1)
441 CCAGT
DpnII 116 GATC ^GATC
165 GATC
EarI 462 CTCTTC CTCTTC(1/4)
79 GAAGAG
HaeIII 3 GGCC GG^CC
156 GGCC
Ksp632I 462 CTCTTC CTCTTC(1/4)
79 GAAGAG
MboI 116 GATC ^GATC
165 GATC
MseI 434 TTAA T^TAA
448 TTAA
NdeII 116 GATC ^GATC
165 GATC
Sau3AI 116 GATC ^GATC
165 GATC
TfiI 183 GATTC G^A[AT]TC
534 GATTC
Tru9I 434 TTAA T^TAA
448 TTAA
Tsp45I 458 GTCAC ^GT[GC]AC
474 GTCAC
TspRI 63 CAGTG CA[GC]TG[ACGT][ACGT]^
381 CAGTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsiYI BslI BsrDI Cfr13I HinfI
HphI MaeIII MboII MnlI NlaIII
NlaIV Sau96I Tsp509I