Restriction map for
pFN21AE6253
(Circular) (Six-base) MAPSORT of: pFN21AE6253.seq Check: 2674 from: 1 to: 5347
pFN21AE6253 5347 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3157 278
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 2690 1937
Size: 166 587 4594
Fragments arranged by size:
4594 587 166
Acc65I G'GTAC_C
Cuts at: 2057 2668 2057
Size: 611 4736
AclI AA'CG_TT
Cuts at: 1563 3474 3847 1563
Size: 1911 373 3063
Fragments arranged by size:
3063 1911 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3410 4794 5062 1632
Size: 1778 1384 268 1917
Fragments arranged by size:
1917 1778 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5150
AflIII A'CryG_T
Cuts at: 1203 4246 5223 1203
Size: 3043 977 1327
Fragments arranged by size:
3043 1327 977
AgeI A'CCGG_T
Cuts at: 4178 4178
Size: 5347
AhdI GACnn_n'nnGTC
Cuts at: 4076 4076
Size: 5347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5347
AlwNI CAG_nnn'CTG
Cuts at: 2048 2173 3090 4662 2048
Size: 125 917 1572 2733
Fragments arranged by size:
2733 1572 917 125
ApaLI G'TGCA_C
Cuts at: 3404 4560 3404
Size: 1156 4191
ApoI r'AATT_y
Cuts at: 1493 2622 2656 2882 3034 5143 1493
Size: 1129 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1129 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 3901 160
Size: 872 2869 1606
Fragments arranged by size:
2869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 1870
Size: 73 729 4545
Fragments arranged by size:
4545 729 73
BamHI G'GATC_C
Cuts at: 2677 3044 2677
Size: 367 4980
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2668 4123 618
Size: 325 217 627 270 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2507 2666 1391
Size: 1116 159 4072
Fragments arranged by size:
4072 1116 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3538 3572 1903
Size: 34 1601 34 3678
Fragments arranged by size:
3678 1601 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2349 3239 4449 2349
Size: 890 1210 3247
Fragments arranged by size:
3247 1210 890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3958 136
Size: 107 122 71 631 605 2286 1525
Fragments arranged by size:
2286 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5342 5342
Size: 5347
BlpI GC'TnA_GC
Cuts at: 2764 2764
Size: 5347
Bme1580I G_kGCm'C
Cuts at: 1790 3408 4564 1790
Size: 1618 1156 2573
Fragments arranged by size:
2573 1618 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5347
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 4036 442
Size: 416 53 83 399 24 1661 958 1753
Fragments arranged by size:
1753 1661 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4007 1239
Size: 2768 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3472 4337 4635 3472
Size: 865 298 4184
Fragments arranged by size:
4184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4010 882
Size: 617 2511 2219
Fragments arranged by size:
2511 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3138 493
Size: 711 1934 2702
Fragments arranged by size:
2702 1934 711
BsaBI GATnn'nnATC
Cuts at: 5341 5341
Size: 5347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3154 3536 4171 275
Size: 53 83 186 936 1621 382 635 1451
Fragments arranged by size:
1621 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2482 3780 4178 4452 4599 5010 2482
Size: 1298 398 274 147 411 2819
Fragments arranged by size:
2819 1298 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 1178
Size: 30 165 30 1086 30 4006
Fragments arranged by size:
4006 1086 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5347
BseYI C'CCAG_C
Cuts at: 2035 4550 5046 2035
Size: 2515 496 2336
Fragments arranged by size:
2515 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4774
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3558 4586 1540
Size: 275 186 796 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3408 3493 4564 3408
Size: 85 1071 4191
Fragments arranged by size:
4191 1071 85
BsmI GAATG_Cn'
Cuts at: 2182 2858 2951 2182
Size: 676 93 4578
Fragments arranged by size:
4578 676 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2507 2666 3408 3493 4564 1391
Size: 399 717 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 399 159 85
BspEI T'CCGG_A
Cuts at: 5010 5010
Size: 5347
BspHI T'CATG_A
Cuts at: 3236 4966 3236
Size: 1730 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 844
Size: 1858 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 1394
Size: 429 1411 3507
Fragments arranged by size:
3507 1411 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 2379 2599 3842 4016 65
Size: 1183 942 189 220 1243 174 1396
Fragments arranged by size:
1396 1243 1183 942 220 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3991 4178 1700
Size: 110 2181 187 2869
Fragments arranged by size:
2869 2181 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5347
BssSI C'ACGA_G
Cuts at: 3407 4419 3407
Size: 1012 4335
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5347
BstBI TT'CG_AA
Cuts at: 4151 4151
Size: 5347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5347
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2644
Size: 5347
BstYI r'GATC_y
Cuts at: 1972 2169 2677 3044 3429 3446 4898 4984 4996
Size: 197 508 367 385 17 1452 86 12
Cuts at: 4996 5342 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 508 385 367 346 197 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 3807
Fragments arranged by size:
3807 939 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4608
Fragments arranged by size:
4608 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2863 3657 3677 2074
Size: 789 794 20 3744
Fragments arranged by size:
3744 794 789 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5312
DraI TTT'AAA
Cuts at: 2651 2651
Size: 5347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 809
Size: 507 3038 1802
Fragments arranged by size:
3038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2794 3683 5312
Size: 54 1425 50 275 18 964 889 1629
Cuts at: 5312 8
Size: 43
Fragments arranged by size:
1629 1425 964 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2794 1537
Size: 275 982 4090
Fragments arranged by size:
4090 982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 2473 3277 1353
Size: 726 394 804 3423
Fragments arranged by size:
3423 804 726 394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3934 4308 4454 387
Size: 1064 138 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3410 4007 4794 5062 1239
Size: 393 1778 597 787 268 1524
Fragments arranged by size:
1778 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2656 3034 2656
Size: 378 4969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5347
FspI TGC'GCA
Cuts at: 3853 3853
Size: 5347
HaeII r_GCGC'y
Cuts at: 1678 4494 1678
Size: 2816 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 3988 4020 4062
Size: 32 135 32 452 32 1932 32 42
Cuts at: 4062 4094 1373
Size: 32 2626
Fragments arranged by size:
2626 1932 452 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2937 669
Size: 988 281 753 246 3079
Fragments arranged by size:
3079 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5347
HpaI GTT'AAC
Cuts at: 2937 2937
Size: 5347
KpnI G_GTAC'C
Cuts at: 2061 2672 2061
Size: 611 4736
MfeI C'AATT_G
Cuts at: 2394 2946 2394
Size: 552 4795
MluI A'CGCG_T
Cuts at: 5223 5223
Size: 5347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4461 4645 1305
Size: 3156 184 2007
Fragments arranged by size:
3156 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3525
Fragments arranged by size:
3525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3305 3664 3823 518
Size: 648 1416 723 359 159 2042
Fragments arranged by size:
2042 1416 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2763 3440 4588 4833 1606
Size: 1157 677 1148 245 2120
Fragments arranged by size:
2120 1157 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 3807
Fragments arranged by size:
3807 939 552 49
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5347
NotI GC'GGCC_GC
Cuts at: 2794 2794
Size: 5347
NspI r_CATG'y
Cuts at: 1132 1229 2705 4250 1132
Size: 97 1476 1545 2229
Fragments arranged by size:
2229 1545 1476 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5347
PciI A'CATG_T
Cuts at: 4246 4246
Size: 5347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5347
PmeI GTTT'AAAC
Cuts at: 2651 2651
Size: 5347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 4929 4961 3416
Size: 32 1481 32 3802
Fragments arranged by size:
3802 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5347
PsiI TTA'TAA
Cuts at: 2917 2917
Size: 5347
PstI C_TGCA'G
Cuts at: 830 2699 830
Size: 1869 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5347
SalI G'TCGA_C
Cuts at: 1936 2689 1936
Size: 753 4594
SapI GCTCTTCn'nnn_
Cuts at: 2079 2079
Size: 5347
SbfI CC_TGCA'GG
Cuts at: 2699 2699
Size: 5347
ScaI AGT'ACT
Cuts at: 3595 5307 3595
Size: 1712 3635
SfcI C'TryA_G
Cuts at: 826 1045 2302 2695 3830 4511 4702 826
Size: 219 1257 393 1135 681 191 1471
Fragments arranged by size:
1471 1257 1135 681 393 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5347
SmaI CCC'GGG
Cuts at: 1872 2674 1872
Size: 802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3451 4352 4614 820
Size: 197 926 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3138 493
Size: 2645 2702
SpeI A'CTAG_T
Cuts at: 152 2401 152
Size: 2249 3098
SphI G_CATG'C
Cuts at: 1132 2705 1132
Size: 1573 3774
SspI AAT'ATT
Cuts at: 5 52 3271 5
Size: 47 3219 2081
Fragments arranged by size:
3219 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5347
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2786 513
Size: 552 939 49 733 3074
Fragments arranged by size:
3074 939 733 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3374 3391 3544 3729 4174 325
Size: 1255 609 1185 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1185 609 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3593 5305 96
Size: 274 80 33 51 1443 1616 1712 138
Fragments arranged by size:
1712 1616 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5307 5339 5307
Size: 32 5315
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5347
XbaI T'CTAG_A
Cuts at: 2683 2683
Size: 5347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5347
XmaI C'CCGG_G
Cuts at: 1870 2672 1870
Size: 802 4545
XmnI GAAnn'nnTTC
Cuts at: 3476 3476
Size: 5347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3155 276
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI