################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstF5I BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TaqI TseI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] AlwI 3 GGATC GGATC(4/5) AocI 235 CCTAAGG CC^T[ACGT]AGG ApoI 31 GAATTT [GA]^AATT[CT] AspHI 586 GAGCTC G[AT]GC[AT]^C AvaI 59 CCCGAG C^[CT]CG[GA]G BalI 887 TGGCCA TGG^CCA BanII 586 GAGCTC G[GA]GC[CT]^C BcnI 776 CCCGG CC^[GC]GG BfaI 913 CTAG C^TAG BglII 249 AGATCT A^GATCT BpmI 583 CTGGAG CTGGAG(16/14) BsaI 677 GGTCTC GGTCTC(1/5) BseRI 640 CTCCTC GAGGAG(10/8) BsiHKAI 586 GAGCTC G[AT]GC[AT]^C BsmBI 906 GAGACG CGTCTC(1/5) BsoBI 59 CCCGAG C^[CT]CG[GA]G Bsp1407I 101 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BspMI 715 ACCTGC ACCTGC(4/8) BsrGI 101 TGTACA T^GTACA BsrI 685 ACTGG ACTGG(1/-1) BstAPI 747 GCATTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 738 GGTGACC G^GT[ACGT]ACC BstPI 738 GGTGACC G^GT[ACGT]ACC BstYI 249 AGATCT [GA]^GATC[CT] Bsu36I 235 CCTAAGG CC^T[ACGT]AGG Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC CfoI 472 GCGC GCG^C DsaI 734 CCGTGG C^C[GA][CT]GG EaeI 887 TGGCCA [CT]^GGCC[GA] EarI 903 GAAGAG CTCTTC(1/4) Eco81I 235 CCTAAGG CC^T[ACGT]AGG EcoO65I 738 GGTGACC G^GT[ACGT]ACC HapII 777 CCGG C^CGG HhaI 472 GCGC GCG^C HinP1I 472 GCGC G^CGC HpaII 777 CCGG C^CGG Ksp632I 903 GAAGAG CTCTTC(1/4) MaeI 913 CTAG C^TAG MaeII 332 ACGT A^CGT MflI 249 AGATCT [GA]^GATC[CT] MluNI 887 TGGCCA TGG^CCA MscI 887 TGGCCA TGG^CCA MseI 934 TTAA T^TAA MspI 777 CCGG C^CGG MwoI 747 GCATTTCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 776 CCCGG CC^[GC]GG NlaIV 508 GGACCC GG[ACGT]^[ACGT]CC PflMI 312 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 404 TCATGA T^CATGA SacI 586 GAGCTC GAGCT^C SspBI 101 TGTACA T^GTACA StuI 426 AGGCCT AGG^CCT Tru9I 934 TTAA T^TAA Van91I 312 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 249 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 189 CCGC CCGC(-3/-1) 363 CCGC AfaI 102 GTAC GT^AC 177 GTAC AluI 231 AGCT AG^CT 587 AGCT AlwNI 282 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 800 CAGACACTG BbvCI 672 GCTGAGG CCTCAGC(-5/-2) 864 GCTGAGG BmyI 586 GAGCTC G[AGT]GC[ACT]^C 754 GTGCCC BsmFI 57 GTCCC GGGAC(10/14) 541 GTCCC Bsp1286I 586 GAGCTC G[AGT]GC[ACT]^C 754 GTGCCC DpnII 4 GATC ^GATC 250 GATC DraII 239 AGGTCCT [GA]G^G[ACGT]CC[CT] 548 AGGTCCT EcoO109I 239 AGGTCCT [GA]G^G[ACGT]CC[CT] 548 AGGTCCT HinfI 154 GAATC G^A[ACGT]TC 870 GAATC MboI 4 GATC ^GATC 250 GATC NdeII 4 GATC ^GATC 250 GATC PpuMI 239 AGGTCCT [GA]G^G[AT]CC[CT] 548 AGGTCCT RsaI 102 GTAC GT^AC 177 GTAC Sau3AI 4 GATC ^GATC 250 GATC TfiI 154 GAATC G^A[AT]TC 870 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AvaII BsaJI BsgI BsiYI BslI BsmAI BssKI BstNI Cfr13I DdeI EcoRII HaeIII HphI MaeIII MboII MnlI MslI MvaI NlaIII Sau96I ScrFI SfaNI Tsp45I Tsp509I TspRI