################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 189 CCGC CCGC(-3/-1) AcsI 31 GAATTT [GA]^AATT[CT] AhdI 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 286 AGCT AG^CT ApoI 31 GAATTT [GA]^AATT[CT] AspEI 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 371 TGGCCA TGG^CCA BbvCI 672 GCTGAGG CCTCAGC(-5/-2) BcnI 776 CCCGG CC^[GC]GG BfaI 196 CTAG C^TAG BglII 476 AGATCT A^GATCT BmyI 754 GTGCCC G[AGT]GC[ACT]^C BsaI 677 GGTCTC GGTCTC(1/5) Bsp1286I 754 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 101 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BsrDI 417 GCAATG GCAATG(2/0) BsrGI 101 TGTACA T^GTACA BssSI 913 CTCGTG CACGAG(-5/-1) BstAPI 747 GCATTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 312 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 476 AGATCT [GA]^GATC[CT] DraII 548 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 391 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 371 TGGCCA [CT]^GGCC[GA] Eam1105I 250 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 548 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 318 CCTTGG C^C[AT][AT]GG HapII 777 CCGG C^CGG HgaI 669 GACGC GACGC(5/10) HpaII 777 CCGG C^CGG MaeI 196 CTAG C^TAG MflI 476 AGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspI 777 CCGG C^CGG MwoI 747 GCATTTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 776 CCCGG CC^[GC]GG PflMI 66 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 548 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 404 TCATGA T^CATGA SfaNI 761 GCATC GCATC(5/9) SmlI 583 CTTGAG C^T[CT][GA]AG SspBI 101 TGTACA T^GTACA StyI 318 CCTTGG C^C[AT][AT]GG Tsp509I 32 AATT ^AATT Van91I 66 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 476 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 102 GTAC GT^AC 177 GTAC BbsI 896 GAAGAC GAAGAC(2/6) 908 GAAGAC BbvI 287 GCTGC GCAGC(8/12) 489 GCTGC BpuAI 896 GAAGAC GAAGAC(2/6) 908 GAAGAC BsaJI 318 CCTTGG C^C[ACGT][ACGT]GG 545 CCCAGG BseRI 127 CTCCTC GAGGAG(10/8) 219 CTCCTC BsgI 388 CTGCAC GTGCAG(16/14) 472 GTGCAG BsmAI 156 GTCTC GTCTC(1/5) 678 GTCTC BsmFI 45 GGGAC GGGAC(10/14) 541 GTCCC BstEII 138 GGTCACC G^GT[ACGT]ACC 738 GGTGACC BstPI 138 GGTCACC G^GT[ACGT]ACC 738 GGTGACC DpnII 477 GATC ^GATC 855 GATC DrdI 147 GACCTGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 669 GACGCTGAGGTC EarI 307 CTCTTC CTCTTC(1/4) 903 GAAGAG EcoO65I 138 GGTCACC G^GT[ACGT]ACC 738 GGTGACC Fnu4HI 287 GCTGC GC^[ACGT]GC 489 GCTGC ItaI 287 GCTGC GC^[ACGT]GC 489 GCTGC Ksp632I 307 CTCTTC CTCTTC(1/4) 903 GAAGAG MboI 477 GATC ^GATC 855 GATC NdeII 477 GATC ^GATC 855 GATC NlaIV 508 GGACCC GG[ACGT]^[ACGT]CC 869 GGAACC RsaI 102 GTAC GT^AC 177 GTAC Sau3AI 477 GATC ^GATC 855 GATC TseI 287 GCTGC G^C[AT]GC 489 GCTGC XcmI 312 CCACCTCCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 602 CCAACAATGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AvaII BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI EcoRII FokI HaeIII HinfI HphI MaeIII MboII MnlI MslI MvaI NlaIII PleI Sau96I ScrFI Tsp45I TspRI