################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 177 GTAC GT^AC AflIII 52 ACACGT A^C[GA][CT]GT Alw44I 717 GTGCAC G^TGCAC AlwNI 895 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 717 GTGCAC G^TGCAC AseI 817 ATTAAT AT^TAAT AsnI 817 ATTAAT AT^TAAT AvaI 59 CCCGAG C^[CT]CG[GA]G AvaII 3 GGACC G^G[AT]CC BanII 645 GGGCTC G[GA]GC[CT]^C BbrPI 714 CACGTG CAC^GTG BbsI 88 GTCTTC GAAGAC(2/6) BglII 299 AGATCT A^GATCT BpuAI 88 GTCTTC GAAGAC(2/6) BsaAI 714 CACGTG [CT]AC^GT[GA] BseRI 906 GAGGAG GAGGAG(10/8) BsmAI 909 GAGAC GTCTC(1/5) BsmI 462 GCATTC GAATGC(1/-1) BsoBI 59 CCCGAG C^[CT]CG[GA]G BsrFI 776 GCCGGC [GA]^CCGG[CT] BstAPI 894 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 615 GGTGACC G^GT[ACGT]ACC BstPI 615 GGTGACC G^GT[ACGT]ACC Cfr10I 776 GCCGGC [GA]^CCGG[CT] DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 797 CCATGG C^C[GA][CT]GG EarI 743 CTCTTC CTCTTC(1/4) EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 615 GGTGACC G^GT[ACGT]ACC FauI 158 CCCGC CCCGC(4/6) HapII 777 CCGG C^CGG HgaI 789 GACGC GACGC(5/10) HpaII 777 CCGG C^CGG Ksp632I 743 CTCTTC CTCTTC(1/4) MseI 818 TTAA T^TAA MspI 777 CCGG C^CGG NaeI 776 GCCGGC GCC^GGC NcoI 797 CCATGG C^CATGG NgoMIV 776 GCCGGC G^CCGGC NspI 728 ACATGC [GA]CATG^[CT] PflMI 726 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 714 CACGTG CAC^GTG PmlI 714 CACGTG CAC^GTG PshBI 817 ATTAAT AT^TAAT PstI 901 CTGCAG CTGCA^G RsaI 177 GTAC GT^AC SfcI 901 CTGCAG C^T[GA][CT]AG Tru9I 818 TTAA T^TAA Tsp509I 480 AATT ^AATT Van91I 726 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 65 AGCT AG^CT 587 AGCT AlwI 677 GATCC GGATC(4/5) 249 GGATC AspHI 541 GTGCTC G[AT]GC[AT]^C 717 GTGCAC BpmI 544 CTCCAG CTGGAG(16/14) 67 CTGGAG BsgI 388 CTGCAC GTGCAG(16/14) 564 CTGCAC BsiHKAI 541 GTGCTC G[AT]GC[AT]^C 717 GTGCAC BspHI 593 TCATGA T^CATGA 785 TCATGA BstF5I 471 CATCC GGATG(2/0) 522 GGATG Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC 776 GCCGGC DdeI 673 CTGAG C^T[ACGT]AG 865 CTGAG DraIII 391 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 748 CACTTCGTG EcoT14I 428 CCTTGG C^C[AT][AT]GG 797 CCATGG FokI 471 CATCC GGATG(9/13) 522 GGATG HinfI 154 GAGTC G^A[ACGT]TC 741 GACTC MwoI 319 GCTGGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 894 GCAGAGGCTGC PleI 741 GACTC GAGTC(4/5) 154 GAGTC RcaI 593 TCATGA T^CATGA 785 TCATGA StyI 428 CCTTGG C^C[AT][AT]GG 797 CCATGG XcmI 236 CCAAGTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 312 CCACTTTGCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstXI BstYI Cfr13I DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XhoII