(Linear) (Six-base) MAPSORT of: KIEE5991.seq Check: 367 from: 1 to: 942 KIEE5991.orf With 182 enzymes: SgfI * December 5, 2014 13:18 .. AflIII A'CryG_T Cuts at: 0 52 942 Size: 52 890 AleI CACnn'nnGTG Cuts at: 0 22 942 Size: 22 920 AlwNI CAG_nnn'CTG Cuts at: 0 900 942 Size: 900 42 ApaLI G'TGCA_C Cuts at: 0 717 942 Size: 717 225 AseI AT'TA_AT Cuts at: 0 818 942 Size: 818 124 AvaI C'yCGr_G Cuts at: 0 59 942 Size: 59 883 BanII G_rGCy'C Cuts at: 0 649 942 Size: 649 293 BbsI GAAGACnn'nnnn_ Cuts at: 0 81 942 Size: 81 861 BglII A'GATC_T Cuts at: 0 299 942 Size: 299 643 Bme1580I G_kGCm'C Cuts at: 0 721 942 Size: 721 221 BmgBI CAC'GTC Cuts at: 0 722 942 Size: 722 220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 529 942 Size: 88 441 413 Fragments arranged by size: 441 413 88 BsaAI yAC'GTr Cuts at: 0 716 942 Size: 716 226 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 921 942 Size: 921 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 549 942 Size: 373 176 393 Fragments arranged by size: 393 373 176 BsiHKAI G_wGCw'C Cuts at: 0 545 721 942 Size: 545 176 221 Fragments arranged by size: 545 221 176 BsmI GAATG_Cn' Cuts at: 0 462 942 Size: 462 480 Bsp1286I G_dGCh'C Cuts at: 0 545 649 721 942 Size: 545 104 72 221 Fragments arranged by size: 545 221 104 72 BspHI T'CATG_A Cuts at: 0 593 785 942 Size: 593 192 157 Fragments arranged by size: 593 192 157 BsrFI r'CCGG_y Cuts at: 0 776 942 Size: 776 166 BstAPI GCAn_nnn'nTGC Cuts at: 0 900 942 Size: 900 42 BstEII G'GTnAC_C Cuts at: 0 615 942 Size: 615 327 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 319 519 942 Size: 243 76 200 423 Fragments arranged by size: 423 243 200 76 BstYI r'GATC_y Cuts at: 0 249 299 676 942 Size: 249 50 377 266 Fragments arranged by size: 377 266 249 50 BtgI C'CryG_G Cuts at: 0 797 942 Size: 797 145 DraIII CAC_nnn'GTG Cuts at: 0 396 753 942 Size: 396 357 189 Fragments arranged by size: 396 357 189 EarI CTCTTCn'nnn_ Cuts at: 0 749 942 Size: 749 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 711 942 Size: 711 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 529 942 Size: 88 441 413 Fragments arranged by size: 441 413 88 EcoO109I rG'GnC_Cy Cuts at: 0 705 942 Size: 705 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 241 273 868 900 942 Size: 241 32 595 32 42 Fragments arranged by size: 595 241 42 32 32 MslI CAynn'nnrTG Cuts at: 0 20 22 172 664 733 942 Size: 20 2 150 492 69 209 Fragments arranged by size: 492 209 150 69 20 2 NaeI GCC'GGC Cuts at: 0 778 942 Size: 778 164 NcoI C'CATG_G Cuts at: 0 797 942 Size: 797 145 NgoMIV G'CCGG_C Cuts at: 0 776 942 Size: 776 166 NspI r_CATG'y Cuts at: 0 732 942 Size: 732 210 PflMI CCAn_nnn'nTGG Cuts at: 0 732 942 Size: 732 210 PfoI T'CCnGG_A Cuts at: 0 41 203 942 Size: 41 162 739 Fragments arranged by size: 739 162 41 PmlI CAC'GTG Cuts at: 0 716 942 Size: 716 226 PstI C_TGCA'G Cuts at: 0 905 942 Size: 905 37 SfcI C'TryA_G Cuts at: 0 901 942 Size: 901 41 StyI C'CwwG_G Cuts at: 0 428 797 942 Size: 428 369 145 Fragments arranged by size: 428 369 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 411 435 942 Size: 411 24 507 Fragments arranged by size: 507 411 24 TatI w'GTAC_w Cuts at: 0 176 942 Size: 176 766 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 243 319 942 Size: 243 76 623 Fragments arranged by size: 623 243 76 Enzymes that do cut: AflIII AleI AlwNI ApaLI AseI AvaI BanII BbsI BglII Bme1580I BmgBI BpmI BsaAI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BstAPI BstEII BstXI BstYI BtgI DraIII EarI EciI Eco57MI EcoO109I Hin4I MslI NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PstI SfcI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI ApaI ApoI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI