Restriction map for
pFN21AE6079
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BglI BglII
BlnI BsaAI BsaBI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstYI BstZ17I Bsu36I
Cac8I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MflI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AciI 233 CCGC CCGC(-3/-1)
AcsI 34 AAATTC [GA]^AATT[CT]
AcyI 442 GGCGCC G[GA]^CG[CT]C
AflII 129 CTTAAG C^TTAAG
AgeI 365 ACCGGT A^CCGGT
AlwI 846 GATCC GGATC(4/5)
ApaI 511 GGGCCC GGGCC^C
ApoI 34 AAATTC [GA]^AATT[CT]
AvaI 928 CCCGGG C^[CT]CG[GA]G
BanI 442 GGCGCC G^G[CT][GA]CC
BbeI 442 GGCGCC GGCGC^C
BbsI 310 GTCTTC GAAGAC(2/6)
BclI 521 TGATCA T^GATCA
BfaI 637 CTAG C^TAG
BfrI 129 CTTAAG C^TTAAG
BlpI 689 GCTCAGC GC^T[ACGT]AGC
BpmI 548 CTCCAG CTGGAG(16/14)
Bpu1102I 689 GCTCAGC GC^T[ACGT]AGC
BpuAI 310 GTCTTC GAAGAC(2/6)
BsaHI 442 GGCGCC G[GA]^CG[CT]C
BsaWI 365 ACCGGT [AT]^CCGG[AT]
BsgI 166 CTGCAC GTGCAG(16/14)
BsmI 334 GAATGC GAATGC(1/-1)
BsoBI 928 CCCGGG C^[CT]CG[GA]G
Bsp1407I 776 TGTACA T^GTACA
BspMI 164 ACCTGC ACCTGC(4/8)
BsrDI 138 CATTGC GCAATG(2/0)
BsrFI 365 ACCGGT [GA]^CCGG[CT]
BsrGI 776 TGTACA T^GTACA
BstEII 594 GGTGACC G^GT[ACGT]ACC
BstPI 594 GGTGACC G^GT[ACGT]ACC
BstXI 584 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 689 GCTCAGC GC^T[ACGT]AGC
CfoI 443 GCGC GCG^C
Cfr10I 365 ACCGGT [GA]^CCGG[CT]
DraI 426 TTTAAA TTT^AAA
EarI 751 CTCTTC CTCTTC(1/4)
EcoO65I 594 GGTGACC G^GT[ACGT]ACC
FbaI 521 TGATCA T^GATCA
HaeII 442 GGCGCC [GA]GCGC^[CT]
HhaI 443 GCGC GCG^C
Hin1I 442 GGCGCC G[GA]^CG[CT]C
HinP1I 443 GCGC G^CGC
KasI 442 GGCGCC G^GCGCC
Ksp632I 751 CTCTTC CTCTTC(1/4)
MaeI 637 CTAG C^TAG
MaeII 115 ACGT A^CGT
MfeI 23 CAATTG C^AATTG
MunI 23 CAATTG C^AATTG
MwoI 349 GCAGCTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 442 GGCGCC GG^CGCC
NcoI 914 CCATGG C^CATGG
PinAI 365 ACCGGT A^CCGGT
PleI 157 GACTC GAGTC(4/5)
PpuMI 923 GGGACCC [GA]G^G[AT]CC[CT]
ScaI 302 AGTACT AGT^ACT
SfaNI 243 GATGC GCATC(5/9)
SfoI 442 GGCGCC GGC^GCC
SmaI 928 CCCGGG CCC^GGG
SmlI 129 CTTAAG C^T[CT][GA]AG
SspBI 776 TGTACA T^GTACA
StuI 710 AGGCCT AGG^CCT
TspRI 609 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 928 CCCGGG C^CCGGG
XmaI 928 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 72 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
334 GAATGCTTTC
BanII 100 GGGCTC G[GA]GC[CT]^C
511 GGGCCC
BcnI 928 CCCGG CC^[GC]GG
929 CCGGG
BmyI 100 GGGCTC G[AGT]GC[ACT]^C
511 GGGCCC
Bsp1286I 100 GGGCTC G[AGT]GC[ACT]^C
511 GGGCCC
BsrI 550 CCAGT ACTGG(1/-1)
788 CCAGT
BstF5I 61 CATCC GGATG(2/0)
320 GGATG
DraII 511 GGGCCCT [GA]G^G[ACGT]CC[CT]
923 GGGACCC
DsaI 851 CCGTGG C^C[GA][CT]GG
914 CCATGG
EcoO109I 511 GGGCCCT [GA]G^G[ACGT]CC[CT]
923 GGGACCC
FokI 61 CATCC GGATG(9/13)
320 GGATG
HapII 366 CCGG C^CGG
929 CCGG
HpaII 366 CCGG C^CGG
929 CCGG
MspI 366 CCGG C^CGG
929 CCGG
NciI 928 CCCGG CC^[GC]GG
929 CCGGG
NspI 170 ACATGC [GA]CATG^[CT]
779 ACATGC
Tsp509I 24 AATT ^AATT
35 AATT
XmnI 72 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
334 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I