################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 199 GTAGAC GT^[AC][GT]AC AocI 864 CCTTAGG CC^T[ACGT]AGG AseI 514 ATTAAT AT^TAAT AsnI 514 ATTAAT AT^TAAT Asp700I 696 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 887 GTGCTC G[AT]GC[AT]^C BanI 478 GGCACC G^G[CT][GA]CC BbsI 700 GTCTTC GAAGAC(2/6) BcgI 276 CGATGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 134 TGATCA T^GATCA BglI 340 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 780 CTCCAG CTGGAG(16/14) BpuAI 700 GTCTTC GAAGAC(2/6) BsaBI 129 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 73 CTCCTC GAGGAG(10/8) BsgI 284 GTGCAG GTGCAG(16/14) BsiHKAI 887 GTGCTC G[AT]GC[AT]^C BsmAI 442 GTCTC GTCTC(1/5) BsmFI 257 GGGAC GGGAC(10/14) BsmI 675 GCATTC GAATGC(1/-1) BspMI 406 GCAGGT ACCTGC(4/8) BsrDI 245 CATTGC GCAATG(2/0) BstXI 230 CCAAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 864 CCTTAGG CC^T[ACGT]AGG EarI 380 CTCTTC CTCTTC(1/4) Eco81I 864 CCTTAGG CC^T[ACGT]AGG EcoT14I 344 CCATGG C^C[AT][AT]GG FbaI 134 TGATCA T^GATCA Ksp632I 380 CTCTTC CTCTTC(1/4) MaeII 608 ACGT A^CGT MamI 129 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 344 CCATGG C^CATGG NdeI 651 CATATG CA^TATG PflMI 786 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 514 ATTAAT AT^TAAT PvuII 218 CAGCTG CAG^CTG SfcI 221 CTGTAG C^T[GA][CT]AG SfiI 339 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 376 AGGCCT AGG^CCT StyI 344 CCATGG C^C[AT][AT]GG Van91I 786 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 782 CCAGCCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 696 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 249 GCGG CCGC(-3/-1) 367 GCGG AfaI 171 GTAC GT^AC 765 GTAC AluI 219 AGCT AG^CT 288 AGCT BmyI 416 GTGCCC G[AGT]GC[ACT]^C 887 GTGCTC BsaJI 344 CCATGG C^C[ACGT][ACGT]GG 791 CCGTGG Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C 887 GTGCTC BsrI 430 ACTGG ACTGG(1/-1) 786 CCAGT Cac8I 475 GCAGGC GC[ACGT]^[ACGT]GC 598 GCAGGC DpnII 135 GATC ^GATC 355 GATC DsaI 344 CCATGG C^C[GA][CT]GG 791 CCGTGG EcoT22I 654 ATGCAT ATGCA^T 673 ATGCAT MboI 135 GATC ^GATC 355 GATC MseI 43 TTAA T^TAA 515 TTAA MspA1I 218 CAGCTG C[AC]G^C[GT]G 365 CAGCGG MwoI 340 GCCTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 418 GCCCTCCTTGC NdeII 135 GATC ^GATC 355 GATC NlaIV 433 GGTTCC GG[ACGT]^[ACGT]CC 478 GGCACC NsiI 654 ATGCAT ATGCA^T 673 ATGCAT PleI 148 GACTC GAGTC(4/5) 488 GACTC RsaI 171 GTAC GT^AC 765 GTAC Sau3AI 135 GATC ^GATC 355 GATC TfiI 834 GATTC G^A[AT]TC 846 GAATC Tru9I 43 TTAA T^TAA 515 TTAA Tsp45I 19 GTGAC ^GT[GC]AC 530 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### BbvI BsiYI BslI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MslI MvaI NlaIII ScrFI SfaNI TseI TspRI