################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AvaII AviII BamHI BanII BbeI BbrPI BcgI BcnI BfrI BglII BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 859 GTCTAC GT^[AC][GT]AC AciI 357 CCGC CCGC(-3/-1) AcsI 919 AAATTT [GA]^AATT[CT] AocI 867 CCTGAGG CC^T[ACGT]AGG ApoI 919 AAATTT [GA]^AATT[CT] Asp700I 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 429 CCTAGG C^CTAGG BalI 314 TGGCCA TGG^CCA BanI 415 GGTGCC G^G[CT][GA]CC BbsI 742 GTCTTC GAAGAC(2/6) BbvCI 215 CCTCAGC CCTCAGC(-5/-2) BciVI 208 GGATAC GTATCC(6/5) BclI 833 TGATCA T^GATCA BlnI 429 CCTAGG C^CTAGG BpmI 783 CTCCAG CTGGAG(16/14) BpuAI 742 GTCTTC GAAGAC(2/6) BssKI 120 CCTGG ^CC[ACGT]GG BstNI 120 CCTGG CC^[AT]GG BstXI 230 CCAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 65 AGATCC [GA]^GATC[CT] Bsu36I 867 CCTGAGG CC^T[ACGT]AGG Cfr13I 426 GGCCC G^G[ACGT]CC DraIII 105 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 867 CCTGAGG CC^T[ACGT]AGG EcoRII 120 CCTGG ^CC[AT]GG FbaI 833 TGATCA T^GATCA HindIII 603 AAGCTT A^AGCTT MflI 65 AGATCC [GA]^GATC[CT] MluNI 314 TGGCCA TGG^CCA MscI 314 TGGCCA TGG^CCA MseI 608 TTAA T^TAA MspA1I 218 CAGCTG C[AC]G^C[GT]G MvaI 120 CCTGG CC^[AT]GG NcoI 794 CCATGG C^CATGG NdeI 437 CATATG CA^TATG NspI 280 GCATGT [GA]CATG^[CT] PflFI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 789 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 148 GACTC GAGTC(4/5) PshAI 148 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 731 CTGCAG CTGCA^G PvuII 218 CAGCTG CAG^CTG Sau96I 426 GGCCC G^G[ACGT]CC ScrFI 120 CCTGG CC^[ACGT]GG SfiI 339 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 574 ACTAGT A^CTAGT TfiI 585 GATTC G^A[AT]TC Tru9I 608 TTAA T^TAA Tth111I 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 789 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 785 CCAGCCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 65 AGATCC [GA]^GATC[CT] XmnI 888 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 66 GATCC GGATC(4/5) 138 GATCC BbvI 364 GCAGC GCAGC(8/12) 774 GCAGC BglI 340 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 418 GCCTGTCTGGC BsrI 47 CCAGT ACTGG(1/-1) 407 CCAGT BstF5I 837 CATCC GGATG(2/0) 125 GGATG DsaI 317 CCACGG C^C[GA][CT]GG 794 CCATGG EaeI 314 TGGCCA [CT]^GGCC[GA] 404 CGGCCA Fnu4HI 364 GCAGC GC^[ACGT]GC 774 GCAGC FokI 837 CATCC GGATG(9/13) 125 GGATG HinfI 148 GACTC G^A[ACGT]TC 585 GATTC ItaI 364 GCAGC GC^[ACGT]GC 774 GCAGC SfaNI 675 GATGC GCATC(5/9) 814 GCATC TseI 364 GCAGC G^C[AT]GC 774 GCAGC Tsp45I 19 GTGAC ^GT[GC]AC 223 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI BfaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI DdeI DpnII EcoT14I EcoT22I HaeIII HphI MaeI MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV NsiI RsaI Sau3AI SfcI StyI Tsp509I TspRI